ERK1/ERK2 Monoclonal Antibody (ERK-7D8) | Invitrogen 13-6200 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ERK1/ERK2 Monoclonal Antibody (ERK-7D8)

catalog :

13-6200

quantity :

200 µg

price :

US 428

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ERK-7D8

reactivity :

human, mouse, rat, dogs, Xenopus laevis

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 44

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; loading ...; fig 5a western blot; human; loading ...; fig 5a	Zhang C, Mao H, Cao Y. Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner. Sci Rep. 2017;7:3769 pubmed publisher
western blot; human; 1:500; loading ...; fig 3a	Yan X, Zhu Z, Xu S, Yang L, Liao X, Zheng M, et al. MicroRNA-140-5p inhibits hepatocellular carcinoma by directly targeting the unique isomerase Pin1 to block multiple cancer-driving pathways. Sci Rep. 2017;7:45915 pubmed publisher
western blot; human; fig 5e	Grugan K, Dorn K, Jarantow S, Bushey B, Pardinas J, Laquerre S, et al. Fc-mediated activity of EGFR x c-Met bispecific antibody JNJ-61186372 enhanced killing of lung cancer cells. MAbs. 2017;9:114-126 pubmed publisher
western blot; human; 1:2000; loading ...; fig 2a	Aguilera O, MuÃ±oz Sagastibelza M, TorrejÃ³n B, Borrero Palacios A, del Puerto Nevado L, MartÃnez Useros J, et al. Vitamin C uncouples the Warburg metabolic switch in KRAS mutant colon cancer. Oncotarget. 2016;7:47954-47965 pubmed publisher
western blot; human; fig 1	Salas E, Roy S, Marsh T, Rubin B, Debnath J. Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell death. Oncogene. 2016;35:2913-22 pubmed publisher
western blot; mouse; 1:500; fig 5b	Murrow L, Malhotra R, Debnath J. ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function. Nat Cell Biol. 2015;17:300-10 pubmed publisher
western blot; human	Bernusso V, Machado Neto J, Pericole F, Vieira K, Duarte A, Traina F, et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta. 2015;1853:388-95 pubmed publisher
western blot; mouse	Janardhan S, Marks R, Gajewski T. Primary murine CD4+ T cells fail to acquire the ability to produce effector cytokines when active Ras is present during Th1/Th2 differentiation. PLoS ONE. 2014;9:e112831 pubmed publisher
western blot; human	Li L, Wu P, Lee J, Li P, Hsieh W, Ho C, et al. Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells. PLoS ONE. 2014;9:e104203 pubmed publisher
western blot; rat; 1:2500; tbl 1	Iio W, Matsukawa N, Tsukahara T, Toyoda A. The effects of oral taurine administration on behavior and hippocampal signal transduction in rats. Amino Acids. 2012;43:2037-46 pubmed publisher
western blot; human	Machado Neto J, Favaro P, Lazarini M, Costa F, Olalla Saad S, Traina F. Knockdown of insulin receptor substrate 1 reduces proliferation and downregulates Akt/mTOR and MAPK pathways in K562 cells. Biochim Biophys Acta. 2011;1813:1404-11 pubmed publisher
western blot; human; fig 1	Urbanet R, Pilon C, Giorgino F, Vettor R, Fallo F. Insulin signaling in adipose tissue of patients with primary aldosteronism. J Endocrinol Invest. 2011;34:86-9 pubmed
western blot; mouse; fig 2a	Zha Y, Marks R, Ho A, Peterson A, Janardhan S, Brown I, et al. T cell anergy is reversed by active Ras and is regulated by diacylglycerol kinase-alpha. Nat Immunol. 2006;7:1166-73 pubmed
western blot; rat; fig 6	Zhu M, Feng J, Lucchinetti E, Fischer G, Xu L, Pedrazzini T, et al. Ischemic postconditioning protects remodeled myocardium via the PI3K-PKB/Akt reperfusion injury salvage kinase pathway. Cardiovasc Res. 2006;72:152-62 pubmed
western blot; rat; fig 7	Lo I, Shih J, Jiang M. Reactive oxygen species and ERK 1/2 mediate monocyte chemotactic protein-1-stimulated smooth muscle cell migration. J Biomed Sci. 2005;12:377-88 pubmed
western blot; rat; 1:5000; fig 2	Tsai M, Jiang M. Extracellular signal-regulated kinase1/2 in contraction of vascular smooth muscle. Life Sci. 2005;76:877-88 pubmed
western blot; rat; fig 5	Usui S, Sugimoto N, Takuwa N, Sakagami S, Takata S, Kaneko S, et al. Blood lipid mediator sphingosine 1-phosphate potently stimulates platelet-derived growth factor-A and -B chain expression through S1P1-Gi-Ras-MAPK-dependent induction of Kruppel-like factor 5. J Biol Chem. 2004;279:12300-11 pubmed
western blot; human; fig 2	Hernandez M, Barrero M, Crespo M, Nieto M. Lysophosphatidic acid inhibits Ca2+ signaling in response to epidermal growth factor receptor stimulation in human astrocytoma cells by a mechanism involving phospholipase C(gamma) and a G(alphai) protein. J Neurochem. 2000;75:1575-82 pubmed
western blot; rat	Chan E, Stang S, Bottorff D, Stone J. Hypothermic stress leads to activation of Ras-Erk signaling. J Clin Invest. 1999;103:1337-44 pubmed
western blot; mouse; fig 1, 2	Korneyev A. Stress-induced tau phosphorylation in mouse strains with different brain Erk 1 + 2 immunoreactivity. Neurochem Res. 1998;23:1539-43 pubmed
immunoprecipitation; mouse western blot; mouse	Webb C, Van Aelst L, Wigler M, Vande Woude G. Signaling pathways in Ras-mediated tumorigenicity and metastasis. Proc Natl Acad Sci U S A. 1998;95:8773-8 pubmed
western blot; Xenopus laevis; 1:1000; fig 1	Duesbery N, Webb C, Leppla S, Gordon V, Klimpel K, Copeland T, et al. Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science. 1998;280:734-7 pubmed
western blot; human; fig 3	Hidari K, Weyrich A, Zimmerman G, McEver R. Engagement of P-selectin glycoprotein ligand-1 enhances tyrosine phosphorylation and activates mitogen-activated protein kinases in human neutrophils. J Biol Chem. 1997;272:28750-6 pubmed
	Dou C, Zhen G, Dan Y, Wan M, Limjunyawong N, Cao X. Sialylation of TLR2 initiates osteoclast fusion. Bone Res. 2022;10:24 pubmed publisher
	Baschieri F, Le Devedec D, Tettarasar S, Elkhatib N, Montagnac G. Frustration of endocytosis potentiates compression-induced receptor signaling. J Cell Sci. 2020;133: pubmed publisher
	Kim S, Hong K, Kim S, Suh H, Jo K. Creatine and taurine mixtures alleviate depressive-like behaviour in Drosophila melanogaster and mice via regulating Akt and ERK/BDNF pathways. Sci Rep. 2020;10:11370 pubmed publisher
	Dong G, Huang X, Jiang S, Ni L, Chen S. Simvastatin Mitigates Apoptosis and Transforming Growth Factor-Beta Upregulation in Stretch-Induced Endothelial Cells. Oxid Med Cell Longev. 2019;2019:6026051 pubmed publisher
	Xuan Z, Wang Y, Xie J. ANO6 promotes cell proliferation and invasion in glioma through regulating the ERK signaling pathway. Onco Targets Ther. 2019;12:6721-6731 pubmed publisher
	Dong G, Lin X, Liu H, Gao D, Cui J, Ren Z, et al. Intermittent hypoxia alleviates increased VEGF and pro-angiogenic potential in liver cancer cells. Oncol Lett. 2019;18:1831-1839 pubmed publisher
	Baschieri F, Dayot S, Elkhatib N, Ly N, Capmany A, Schauer K, et al. Frustrated endocytosis controls contractility-independent mechanotransduction at clathrin-coated structures. Nat Commun. 2018;9:3825 pubmed publisher
	Areiza Mazo N, Robles J, Zamudio Rodriguez J, Giraldez L, Echeverria V, Barrera Bailón B, et al. Extracts of Physalis peruviana Protect Astrocytic Cells Under Oxidative Stress With Rotenone. Front Chem. 2018;6:276 pubmed publisher
	Araújo A, Araújo L, Medeiros C, Leitao R, Brito G, Costa D, et al. Protective effect of angiotensin II receptor blocker against oxidative stress and inflammation in an oral mucositis experimental model. J Oral Pathol Med. 2018;47:972-984 pubmed publisher
	Blas Rus N, Bustos Morán E, Perez de Castro I, de Carcer G, Borroto A, Camafeita E, et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun. 2016;7:11389 pubmed publisher
	Mòdol L, Mancuso R, Alé A, Francos Quijorna I, Navarro X. Differential effects on KCC2 expression and spasticity of ALS and traumatic injuries to motoneurons. Front Cell Neurosci. 2014;8:7 pubmed publisher
	Montenegro L, Leal A, Coutinho D, Valassi H, Nishi M, Arnhold I, et al. Post-receptor IGF1 insensitivity restricted to the MAPK pathway in a Silver-Russell syndrome patient with hypomethylation at the imprinting control region on chromosome 11. Eur J Endocrinol. 2012;166:543-50 pubmed publisher
	Janardhan S, Praveen K, Marks R, Gajewski T. Evidence implicating the Ras pathway in multiple CD28 costimulatory functions in CD4+ T cells. PLoS ONE. 2011;6:e24931 pubmed publisher
	Driessens G, Zheng Y, Locke F, Cannon J, Gounari F, Gajewski T. Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-?1 phosphorylation. J Immunol. 2011;186:784-90 pubmed publisher
	Yin G, Zheng Q, Yan C, Berk B. GIT1 is a scaffold for ERK1/2 activation in focal adhesions. J Biol Chem. 2005;280:27705-12 pubmed
	Michlig S, Mercier A, Doucet A, Schild L, Horisberger J, Rossier B, et al. ERK1/2 controls Na,K-ATPase activity and transepithelial sodium transport in the principal cell of the cortical collecting duct of the mouse kidney. J Biol Chem. 2004;279:51002-12 pubmed
	Natalicchio A, Laviola L, De Tullio C, Renna L, Montrone C, Perrini S, et al. Role of the p66Shc isoform in insulin-like growth factor I receptor signaling through MEK/Erk and regulation of actin cytoskeleton in rat myoblasts. J Biol Chem. 2004;279:43900-9 pubmed
	Martinez Quiles N, Ho H, Kirschner M, Ramesh N, Geha R. Erk/Src phosphorylation of cortactin acts as a switch on-switch off mechanism that controls its ability to activate N-WASP. Mol Cell Biol. 2004;24:5269-80 pubmed
	Derkinderen P, Valjent E, Toutant M, Corvol J, Enslen H, Ledent C, et al. Regulation of extracellular signal-regulated kinase by cannabinoids in hippocampus. J Neurosci. 2003;23:2371-82 pubmed
	Carraway C, Carvajal M, Carraway K. Association of the Ras to mitogen-activated protein kinase signal transduction pathway with microfilaments. Evidence for a p185(neu)-containing cell surface signal transduction particle linking the mitogenic pathway to a membrane-microfilament associ. J Biol Chem. 1999;274:25659-67 pubmed
	Bird T, Sleath P, DeRoos P, Dower S, Virca G. Interleukin-1 represents a new modality for the activation of extracellular signal-regulated kinases/microtubule-associated protein-2 kinases. J Biol Chem. 1991;266:22661-70 pubmed

product information

Product Type :

Antibody

Product Name :

ERK1/ERK2 Monoclonal Antibody (ERK-7D8)

Catalog # :

13-6200

Quantity :

200 µg

Price :

US 428

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rat

Applications :

ELISA: 0.1-1 mg/mL, Gel Shift: Assay-dependent, Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 2-5 µg, Western Blot: 1 µg/mL

Species :

Canine, Human, Mouse, Rat

Clone :

ERK-7D8

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

Immunogen :

Synthetic peptide (aa 324-345) corresponding to a segment near the C-terminus of rat ERK1

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1 mg/mL, Gel Shift: Assay-dependent, Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 2-5 µg, Western Blot: 1 µg/mL

Aliases :

12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; CG18732; CT34260; CT39192; DERK; D-ERK; DERK-A; Diphospho-ERK; dm-dpERK; Dmel CG12559; Dmel_CG12559; DmErk; DmERKA; DmERK-A; DmMAPK; dpERK; dp-ERK; dpERK1; dpMAPK; drosophila ERK; Dsor2; E(sina)7; EC2-1; EK2-1; enhancer of seven in absentia 7; ERK; Erk MAP kinase; Erk/Map kinase; ERK/rolled; Erk1; Erk-1; erk1 erk2; Erk1/2; ERK1b; ERK2; ERK-2; ERKA; ERK-A; ERT1; ERT2; Esrk1; extracellular signal-regulated kinase; extracellular signal-regulated kinase (ERK2); extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-regulated kinase-1; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; EY2-2; fi06b09; GroupII; HS44KDAP; HUMKER1A; I79_009500; I79_018350; insulin-stimulated MAP2 kinase; l(2)41Ac; l(2R)EMS45-39; M phase MAP kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MapK; MAP-k; MAPK 1; MAPK 2; MAPK 3; MAPK1; mapk1.S; mapk1a; mapk1-a; mapk1-b; mapk2; mapk3; MAP-kinase; MBP kinase; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen-activated 3; mitogen-activated protein kinase; mitogen-activated protein kinase 1; mitogen-activated protein kinase 1 S homeolog; mitogen-activated protein kinase 1a; mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; mpk1; Mtap2k; Myelin basic protein kinase; Myelin xP42 protein kinase; p38; p40; p41; p41mapk; p42; p42 MAP Kinase; P42MAPK; p42-MAPK; p44; p44 MAP kinase; p44erk1; p44-ERK1; p44mapk; p44-MAPK; pERK; p-ERK; phospho-ERK; pMapK; pp42/MAP kinase; Prkm1; PRKM2; Prkm3; protein kinase; Protein rolled; protein tyrosine kinase ERK2; RE08694p; RL; rl/ERK; rl/MAPK; rll; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; rolled; sem; sevenmaker; SR2-1; Su(Raf)2B; wu:fi06b09; XELAEV_18010534mg; xp42; zERK1; zERK2

more info or order :

Invitrogen product webpage