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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
E-cadherin Monoclonal Antibody (SHE78-7)
catalog :
13-5700
quantity :
100µg
price :
US 409
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SHE78-7
reactivity :
human, mouse, cow
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 50
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; loading ...; fig 7i
Swain S, Roe M, Sebrell T, Sidar B, Dankoff J, VanAusdol R, et al. CD103 (αE Integrin) Undergoes Endosomal Trafficking in Human Dendritic Cells, but Does Not Mediate Epithelial Adhesion. Front Immunol. 2018;9:2989 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 6d
Ben Ismail M, Carreiras F, Agniel R, Mili D, Sboui D, Zanina N, et al. Application of APTES-Anti-E-cadherin film for early cancer monitoring. Colloids Surf B Biointerfaces. 2016;146:550-7 pubmed publisher
  • western blot; human; fig 7
Kajiho H, Kajiho Y, Frittoli E, Confalonieri S, Bertalot G, Viale G, et al. RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs. EMBO Rep. 2016;17:1061-80 pubmed publisher
  • western blot; human; fig 1
Schmidt T, Perna A, Fugmann T, Böhm M, Jan Hiss -, Haller S, et al. Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA. Sci Rep. 2016;6:23264 pubmed publisher
  • blocking or activating experiments; human; fig 5
Goyer M, Loiselet A, Bon F, L Ollivier C, Laue M, Holland G, et al. Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier. PLoS ONE. 2016;11:e0149159 pubmed publisher
  • immunocytochemistry; human
Rayavarapu R, Heiden B, Pagani N, Shaw M, Shuff S, Zhang S, et al. The role of multicellular aggregation in the survival of ErbB2-positive breast cancer cells during extracellular matrix detachment. J Biol Chem. 2015;290:8722-33 pubmed publisher
  • western blot; human; loading ...; fig 1b
Fenton S, Hutchens K, Denning M. Targeting Fyn in Ras-transformed cells induces F-actin to promote adherens junction-mediated cell-cell adhesion. Mol Carcinog. 2015;54:1181-93 pubmed publisher
  • blocking or activating experiments; human
Truffi M, Dubreuil V, Liang X, Vacaresse N, Nigon F, Han S, et al. RPTP? controls epithelial adherens junctions, linking E-cadherin engagement to c-Src-mediated phosphorylation of cortactin. J Cell Sci. 2014;127:2420-32 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
Weiswald L, Richon S, Massonnet G, Guinebretiere J, Vacher S, Laurendeau I, et al. A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation. Br J Cancer. 2013;108:1720-31 pubmed publisher
  • western blot; human; fig 5
Boyd S, Mijatov B, Pupo G, Tran S, Gowrishankar K, Shaw H, et al. Oncogenic B-RAF(V600E) signaling induces the T-Box3 transcriptional repressor to repress E-cadherin and enhance melanoma cell invasion. J Invest Dermatol. 2013;133:1269-77 pubmed publisher
  • immunocytochemistry; human; fig 3
Lawrence B, Pan Z, Rosenblatt M. Silk film topography directs collective epithelial cell migration. PLoS ONE. 2012;7:e50190 pubmed publisher
  • blocking or activating experiments; human; 2 ug/ml
Garnier D, Magnus N, Lee T, Bentley V, Meehan B, Milsom C, et al. Cancer cells induced to express mesenchymal phenotype release exosome-like extracellular vesicles carrying tissue factor. J Biol Chem. 2012;287:43565-72 pubmed publisher
  • immunocytochemistry; human; 5 ug/ml; fig 3
Lu L, Zhou D, Jiang X, Song K, Li K, Ding W. Loss of E-cadherin in multidrug resistant breast cancer cell line MCF-7/Adr: possible implication in the enhanced invasive ability. Eur Rev Med Pharmacol Sci. 2012;16:1271-9 pubmed
  • immunoprecipitation; human; fig 5
  • immunocytochemistry; human; fig 6
Troyanovsky R, Klingelhofer J, Troyanovsky S. ?-Catenin contributes to the strength of E-cadherin-p120 interactions. Mol Biol Cell. 2011;22:4247-55 pubmed publisher
  • western blot; human; fig s1b
Hong S, Troyanovsky R, Troyanovsky S. Cadherin exits the junction by switching its adhesive bond. J Cell Biol. 2011;192:1073-83 pubmed publisher
  • flow cytometry; mouse; fig 1
Spencer H, Keramari M, Ward C. Using cadherin expression to assess spontaneous differentiation of embryonic stem cells. Methods Mol Biol. 2011;690:81-94 pubmed publisher
  • blocking or activating experiments; human; 2 ug/ml
Mohamet L, Lea M, Ward C. Abrogation of E-cadherin-mediated cellular aggregation allows proliferation of pluripotent mouse embryonic stem cells in shake flask bioreactors. PLoS ONE. 2010;5:e12921 pubmed publisher
  • blocking or activating experiments; human; 1:50; fig s2
Smutny M, Cox H, Leerberg J, Kovacs E, Conti M, Ferguson C, et al. Myosin II isoforms identify distinct functional modules that support integrity of the epithelial zonula adherens. Nat Cell Biol. 2010;12:696-702 pubmed publisher
  • western blot; human; fig 5b
Zhou Y, Chen S, Ju J, Shen L, Liu Y, Zhen S, et al. Tumor suppressor function of BCSC-1 in nasopharyngeal carcinoma. Cancer Sci. 2009;100:1817-22 pubmed publisher
  • blocking or activating experiments; human; 4 mg/ml; fig 3A
den Elzen N, Buttery C, Maddugoda M, Ren G, Yap A. Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia. Mol Biol Cell. 2009;20:3740-50 pubmed publisher
  • blocking or activating experiments; human; 5 ug/ml
Fournier M, Fata J, Martin K, Yaswen P, Bissell M. Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells. Cancer Res. 2009;69:4545-52 pubmed publisher
  • immunocytochemistry; human; 1 ug/ml; fig 2
  • western blot; human; fig 2
Borley A, Hiscox S, Gee J, Smith C, Shaw V, Barrett Lee P, et al. Anti-oestrogens but not oestrogen deprivation promote cellular invasion in intercellular adhesion-deficient breast cancer cells. Breast Cancer Res. 2008;10:R103 pubmed publisher
  • immunocytochemistry; human; fig 5
Marín Briggiler C, Veiga M, Matos M, Echeverría M, Furlong L, Vazquez Levin M. Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization. Mol Hum Reprod. 2008;14:561-71 pubmed publisher
  • immunoprecipitation; human; fig 4
Kiss A, Troyanovsky R, Troyanovsky S. p120-catenin is a key component of the cadherin-gamma-secretase supercomplex. Mol Biol Cell. 2008;19:4042-50 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
Bryan R, Atherfold P, Yeo Y, Jones L, Harrison R, Wallace D, et al. Cadherin switching dictates the biology of transitional cell carcinoma of the bladder: ex vivo and in vitro studies. J Pathol. 2008;215:184-94 pubmed publisher
  • blocking or activating experiments; human
Haga T, Uchide N, Tugizov S, Palefsky J. Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation. Apoptosis. 2008;13:97-108 pubmed
  • immunocytochemistry; human; fig 5
Troyanovsky R, Laur O, Troyanovsky S. Stable and unstable cadherin dimers: mechanisms of formation and roles in cell adhesion. Mol Biol Cell. 2007;18:4343-52 pubmed
  • flow cytometry; human
Tabata T, McDonagh S, Kawakatsu H, Pereira L. Cytotrophoblasts infected with a pathogenic human cytomegalovirus strain dysregulate cell-matrix and cell-cell adhesion molecules: a quantitative analysis. Placenta. 2007;28:527-37 pubmed
  • western blot; human; fig 6
Liu Z, Xiao M, Balint K, Smalley K, Brafford P, Qiu R, et al. Notch1 signaling promotes primary melanoma progression by activating mitogen-activated protein kinase/phosphatidylinositol 3-kinase-Akt pathways and up-regulating N-cadherin expression. Cancer Res. 2006;66:4182-90 pubmed
  • blocking or activating experiments; human; 2 ug/ml; fig 6
Dalrymple S, Antony L, Xu Y, Uzgare A, Arnold J, Savaugeot J, et al. Role of notch-1 and E-cadherin in the differential response to calcium in culturing normal versus malignant prostate cells. Cancer Res. 2005;65:9269-79 pubmed
  • western blot; human; fig 5
Lu S, Yu G, Zhu Y, Archer M. Cyclooxygenase-2 overexpression in MCF-10F human breast epithelial cells inhibits proliferation, apoptosis and differentiation, and causes partial transformation. Int J Cancer. 2005;116:847-52 pubmed
  • western blot; human; 2 ug/ml; fig 8
Motti M, Califano D, Baldassarre G, Celetti A, Merolla F, Forzati F, et al. Reduced E-cadherin expression contributes to the loss of p27kip1-mediated mechanism of contact inhibition in thyroid anaplastic carcinomas. Carcinogenesis. 2005;26:1021-34 pubmed
  • immunohistochemistry - paraffin section; human; 1:5; fig 2
Sedivy R, Peters K, Kloppel G. Osteopontin expression in ductal adenocarcinomas and undifferentiated carcinomas of the pancreas. Virchows Arch. 2005;446:41-5 pubmed
  • flow cytometry; human; fig 2
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 1
Li G, Fukunaga M, Herlyn M. Reversal of melanocytic malignancy by keratinocytes is an E-cadherin-mediated process overriding beta-catenin signaling. Exp Cell Res. 2004;297:142-51 pubmed
  • western blot; human; 1:1000; fig 1
Mérot Y, Métivier R, Penot G, Manu D, Saligaut C, Gannon F, et al. The relative contribution exerted by AF-1 and AF-2 transactivation functions in estrogen receptor alpha transcriptional activity depends upon the differentiation stage of the cell. J Biol Chem. 2004;279:26184-91 pubmed
  • blocking or activating experiments; human; 1 ug/ml
Mariner D, Davis M, Reynolds A. EGFR signaling to p120-catenin through phosphorylation at Y228. J Cell Sci. 2004;117:1339-50 pubmed
  • immunocytochemistry; human; 1 ug/ml; fig 4
Davis M, Ireton R, Reynolds A. A core function for p120-catenin in cadherin turnover. J Cell Biol. 2003;163:525-34 pubmed
  • flow cytometry; human
  • western blot; human
Li G, Satyamoorthy K, Herlyn M. N-cadherin-mediated intercellular interactions promote survival and migration of melanoma cells. Cancer Res. 2001;61:3819-25 pubmed
  • immunohistochemistry - frozen section; cow
  • immunohistochemistry; cow
Burke J, Cao F, Irving P. High levels of E-/P-cadherin: correlation with decreased apical polarity of Na/K ATPase in bovine RPE cells in situ. Invest Ophthalmol Vis Sci. 2000;41:1945-52 pubmed
  • blocking or activating experiments; human
Hsu M, Meier F, Nesbit M, Hsu J, Van Belle P, Elder D, et al. E-cadherin expression in melanoma cells restores keratinocyte-mediated growth control and down-regulates expression of invasion-related adhesion receptors. Am J Pathol. 2000;156:1515-25 pubmed
  • blocking or activating experiments; human
Taraszka K, Higgins J, Tan K, Mandelbrot D, Wang J, Brenner M. Molecular basis for leukocyte integrin alpha(E)beta(7) adhesion to epithelial (E)-cadherin. J Exp Med. 2000;191:1555-67 pubmed
  • western blot; human; fig 4
Vallorosi C, Day K, Zhao X, Rashid M, Rubin M, Johnson K, et al. Truncation of the beta-catenin binding domain of E-cadherin precedes epithelial apoptosis during prostate and mammary involution. J Biol Chem. 2000;275:3328-34 pubmed
  • blocking or activating experiments; human
Mueller S, Cadenas E, Schonthal A. p21WAF1 regulates anchorage-independent growth of HCT116 colon carcinoma cells via E-cadherin expression. Cancer Res. 2000;60:156-63 pubmed
  • blocking or activating experiments; human
Hines M, Jin H, Wheelock M, Jensen P. Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes. J Cell Sci. 1999;112 ( Pt 24):4569-79 pubmed
  • blocking or activating experiments; human
Day M, Zhao X, Vallorosi C, Putzi M, Powell C, Lin C, et al. E-cadherin mediates aggregation-dependent survival of prostate and mammary epithelial cells through the retinoblastoma cell cycle control pathway. J Biol Chem. 1999;274:9656-64 pubmed
  • blocking or activating experiments; mouse
St Croix B, Sheehan C, Rak J, Flørenes V, Slingerland J, Kerbel R. E-Cadherin-dependent growth suppression is mediated by the cyclin-dependent kinase inhibitor p27(KIP1). J Cell Biol. 1998;142:557-71 pubmed
  • blocking or activating experiments; human
Kantak S, Kramer R. E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells. J Biol Chem. 1998;273:16953-61 pubmed
Xiang X, Phung Y, Feng M, Nagashima K, Zhang J, Broaddus V, et al. The development and characterization of a human mesothelioma in vitro 3D model to investigate immunotoxin therapy. PLoS ONE. 2011;6:e14640 pubmed publisher
Yates C, Shepard C, Stolz D, Wells A. Co-culturing human prostate carcinoma cells with hepatocytes leads to increased expression of E-cadherin. Br J Cancer. 2007;96:1246-52 pubmed
Liu W, Nelson C, Pirone D, Chen C. E-cadherin engagement stimulates proliferation via Rac1. J Cell Biol. 2006;173:431-41 pubmed
product information
Product Type :
Antibody
Product Name :
E-cadherin Monoclonal Antibody (SHE78-7)
Catalog # :
13-5700
Quantity :
100µg
Price :
US 409
Clonality :
Monoclonal
Purity :
Ion-exchange chromatography
Host :
Mouse
Reactivity :
Human
Applications :
ELISA: 2 µg/mL, Flow Cytometry: 1 µg/mL, Immunohistochemistry (Frozen): 2 µg/mL, Immunohistochemistry: 2 µg/mL, Inhibition Assays: 1 µg/mL, Western Blot: 2 µg/mL
Species :
Human
Clone :
SHE78-7
Isotype :
IgG2a
Storage :
4° C
Description :
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
Immunogen :
Human placenta.
Format :
Lyophilized
Applications w/Dilutions :
ELISA: 2 µg/mL, Flow Cytometry: 1 µg/mL, Immunohistochemistry (Frozen): 2 µg/mL, Immunohistochemistry: 2 µg/mL, Inhibition Assays: 1 µg/mL, Western Blot: 2 µg/mL
Aliases :
Arc-1, CD324, CDHE, ECAD, LCAM, UVO, CAM 120/80, E-Cadherin, cadherin 1, E-cadherin epithelial, cadherin-1, calcium-dependent adhesion protein, epithelial, cell-CAM 120/80, epithelial cadherin, uvomorulin, cadherin-E
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA