STAT5 beta Monoclonal Antibody (ST5b-10G1) | Invitrogen 13-5300 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

STAT5 beta Monoclonal Antibody (ST5b-10G1)

catalog :

13-5300

quantity :

100µg

price :

US 345.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ST5b-10G1

reactivity :

human, mouse, rat, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - free floating section, ChIP-Seq

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 4a	Goldstein J, Burlion A, Zaragoza B, Sendeyo K, Polansky J, Huehn J, et al. Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression. PLoS ONE. 2016;11:e0153682 pubmed publisher
EMSA; bovine; fig 6	Nguyen M, Boutinaud M, PÃ©tridou B, Gabory A, Pannetier M, Chat S, et al. DNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking. PLoS ONE. 2014;9:e111556 pubmed publisher
ChIP-Seq; human; 12 ug immunocytochemistry; human; 1:1000 western blot; human	Kanai T, Seki S, Jenks J, Kohli A, Kawli T, Martin D, et al. Identification of STAT5A and STAT5B target genes in human T cells. PLoS ONE. 2014;9:e86790 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:3000; fig 3	Yip S, Eguchi R, Grattan D, Bunn S. Prolactin signalling in the mouse hypothalamus is primarily mediated by signal transducer and activator of transcription factor 5b but not 5a. J Neuroendocrinol. 2012;24:1484-91 pubmed publisher
immunoprecipitation; human western blot; human	Nelson E, Walker S, Li W, Liu X, Frank D. Identification of human STAT5-dependent gene regulatory elements based on interspecies homology. J Biol Chem. 2006;281:26216-24 pubmed
western blot; mouse	Nelson E, Walker S, Alvarez J, Frank D. Isolation of unique STAT5 targets by chromatin immunoprecipitation-based gene identification. J Biol Chem. 2004;279:54724-30 pubmed
immunoprecipitation; human western blot; human; 1:500	Cotarla I, Ren S, Zhang Y, Gehan E, Singh B, Furth P. Stat5a is tyrosine phosphorylated and nuclear localized in a high proportion of human breast cancers. Int J Cancer. 2004;108:665-71 pubmed
	Hsieh W, Hsu T, Chang Y, Lai M. IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells. Nat Commun. 2018;9:463 pubmed publisher
	Silver D, Naora H, Liu J, Cheng W, Montell D. Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility. Cancer Res. 2004;64:3550-8 pubmed
	Chong M, Cornish A, Darwiche R, Stanley E, Purton J, Godfrey D, et al. Suppressor of cytokine signaling-1 is a critical regulator of interleukin-7-dependent CD8+ T cell differentiation. Immunity. 2003;18:475-87 pubmed
	Millot B, Montoliu L, Fontaine M, Mata T, Devinoy E. Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes. Biochem J. 2003;372:41-52 pubmed

product information

Product Type :

Antibody

Product Name :

STAT5 beta Monoclonal Antibody (ST5b-10G1)

Catalog # :

13-5300

Quantity :

100µg

Price :

US 345.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ChIP assay: 5 µg/mL, ELISA: Assay Dependent, Gel Shift: Assay Dependent, Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunohistochemistry (Paraffin): 1:20-1:200, Immunoprecipitation: Assay Dependent, Western Blot: 1:250

Species :

Human, Mouse, Rat

Clone :

ST5b-10G1

Isotype :

IgG2a

Storage :

-20°C

Description :

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Immunogen :

A peptide corresponding to the unique carboxy-terminus of murine STAT5b coupled to a protein carrier.

Format :

Liquid

Applications w/Dilutions :

Aliases :

BOS_19070; LOC101122961; mammary gland factor STAT5B; Signal transducer and activator of transcription 5a; Signal transducer and activator of transcription 5B; STA5B; STAT; STAT5; STAT5B; transcription factor STAT5B

more info or order :

Invitrogen product webpage