Perlecan Monoclonal Antibody (7B5) | Invitrogen 13-4400 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Perlecan Monoclonal Antibody (7B5)

catalog :

13-4400

quantity :

100µg

price :

US 345.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7B5

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; 1:100; fig 4a	Caberlotto E, Ruiz L, Miller Z, Poletti M, Tadlock L. Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles. PLoS ONE. 2017;12:e0172624 pubmed publisher
western blot; human; 1:500; fig 6	FullÃ¡r A, DudÃ¡s J, OlÃ¡h L, HollÃ³si P, Papp Z, Sobel G, et al. Remodeling of extracellular matrix by normal and tumor-associated fibroblasts promotes cervical cancer progression. BMC Cancer. 2015;15:256 pubmed publisher
immunohistochemistry - frozen section; human	Bauché S, Boerio D, Davoine C, Bernard V, Stum M, Bureau C, et al. Peripheral nerve hyperexcitability with preterminal nerve and neuromuscular junction remodeling is a hallmark of Schwartz-Jampel syndrome. Neuromuscul Disord. 2013;23:998-1009 pubmed publisher
immunohistochemistry - frozen section; human; 1:10; fig 10 ELISA; human; 2 ug/ml; fig 1	Kennett E, Rees M, Malle E, Hammer A, Whitelock J, Davies M. Peroxynitrite modifies the structure and function of the extracellular matrix proteoglycan perlecan by reaction with both the protein core and the heparan sulfate chains. Free Radic Biol Med. 2010;49:282-93 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; tbl 2	Liu Y, Carson Walter E, Cooper A, Winans B, Johnson M, Walter K. Vascular gene expression patterns are conserved in primary and metastatic brain tumors. J Neurooncol. 2010;99:13-24 pubmed publisher
immunocytochemistry; human; 1:500; fig 5	Akerud A, Dubicke A, Sennstrom M, Ekman Ordeberg G, Malmstrom A. Differences in heparan sulfate production in cervical fibroblast cultures from women undergoing term and preterm delivery. Acta Obstet Gynecol Scand. 2008;87:1220-8 pubmed publisher
immunohistochemistry; human; fig 4 western blot; human; fig 6	Melrose J, Roughley P, Knox S, Smith S, Lord M, Whitelock J. The structure, location, and function of perlecan, a prominent pericellular proteoglycan of fetal, postnatal, and mature hyaline cartilages. J Biol Chem. 2006;281:36905-14 pubmed
	Wimmer R, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature. 2019;565:505-510 pubmed publisher
	HedstrÃ¶m U, Hallgren O, Ã–berg L, DeMicco A, Vaarala O, Westergren Thorsson G, et al. Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells. Sci Rep. 2018;8:3502 pubmed publisher
	Botta A, Delteil F, Mettouchi A, Vieira A, Estrach S, Negroni L, et al. Confluence switch signaling regulates ECM composition and the plasmin proteolytic cascade in keratinocytes. J Cell Sci. 2012;125:4241-52 pubmed publisher
	Behrens D, Villone D, Koch M, Brunner G, Sorokin L, Robenek H, et al. The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens. J Biol Chem. 2012;287:18700-9 pubmed publisher
	Ellis A, Pan W, Yang G, Jones K, Chuang C, Whitelock J, et al. Similarity of recombinant human perlecan domain 1 by alternative expression systems bioactive heterogenous recombinant human perlecan D1. BMC Biotechnol. 2010;10:66 pubmed publisher
	Stratman A, Malotte K, Mahan R, Davis M, Davis G. Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation. Blood. 2009;114:5091-101 pubmed publisher
	Björnson A, Moses J, Ingemansson A, Haraldsson B, Sorensson J. Primary human glomerular endothelial cells produce proteoglycans, and puromycin affects their posttranslational modification. Am J Physiol Renal Physiol. 2005;288:F748-56 pubmed
	Evans M, Fanucchi M, Baker G, Van Winkle L, Pantle L, Nishio S, et al. Atypical development of the tracheal basement membrane zone of infant rhesus monkeys exposed to ozone and allergen. Am J Physiol Lung Cell Mol Physiol. 2003;285:L931-9 pubmed
	Evans M, Fanucchi M, Van Winkle L, Baker G, Murphy A, Nishio S, et al. Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone. Am J Physiol Lung Cell Mol Physiol. 2002;283:L1263-70 pubmed

product information

Product Type :

Antibody

Product Name :

Perlecan Monoclonal Antibody (7B5)

Catalog # :

13-4400

Quantity :

100µg

Price :

US 345.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay Dependent, Immunohistochemistry (Paraffin): 1:10-1:50, Western Blot: Assay Dependent

Species :

Human

Clone :

7B5

Isotype :

IgG1

Storage :

-20°C

Description :

Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Immunogen :

rec-Perlecan domain III.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay Dependent, Immunohistochemistry (Paraffin): 1:10-1:50, Western Blot: Assay Dependent

Aliases :

AI852380; Basement membrane-specific heparan sulfate proteoglycan core protein; BOS_2497; Endorepellin; endorepellin (domain V region); heparan sulfate proteoglycan 2; HSPG; HSPG2; LG3 peptide; LOC313641; Pcn; per; Perlecan; perlecan (heparan sulfate proteoglycan 2); perlecan proteoglycan; PLC; PRCAN; RP11-132G19.2; SJA; SJS; SJS1

more info or order :

Invitrogen product webpage