p53 Monoclonal Antibody (PAb 240) | Invitrogen 13-4100 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p53 Monoclonal Antibody (PAb 240)

catalog :

13-4100

quantity :

100 µg

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAb 240

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; fig 5	Liu Z, Wan G, Heaphy C, Bisoffi M, Griffith J, Hu C. A novel loss-of-function mutation in TP53 in an endometrial cancer cell line and uterine papillary serous carcinoma model. Mol Cell Biochem. 2007;297:179-87 pubmed
western blot; human; 1:1000; fig 4	Nim S, Jeon J, Corbi Verge C, Seo M, Ivarsson Y, Moffat J, et al. Pooled screening for antiproliferative inhibitors of protein-protein interactions. Nat Chem Biol. 2016;12:275-81 pubmed publisher
western blot; mouse; fig 2	Kumar P P, Emechebe U, Smith R, Franklin S, Moore B, Yandell M, et al. Coordinated control of senescence by lncRNA and a novel T-box3 co-repressor complex. elife. 2014;3: pubmed publisher
immunohistochemistry; mouse	El Ashmawy N, El Bahrawy H, Shamloula M, El Feky O. Biochemical/metabolic changes associated with hepatocellular carcinoma development in mice. Tumour Biol. 2014;35:5459-66 pubmed publisher
western blot; mouse; fig 3	Napoli E, Ross Inta C, Wong S, Hung C, Fujisawa Y, Sakaguchi D, et al. Mitochondrial dysfunction in Pten haplo-insufficient mice with social deficits and repetitive behavior: interplay between Pten and p53. PLoS ONE. 2012;7:e42504 pubmed publisher
immunohistochemistry; mouse; 1:250; fig s1	Rebel H, Bodmann C, van de Glind G, de Gruijl F. UV-induced ablation of the epidermal basal layer including p53-mutant clones resets UV carcinogenesis showing squamous cell carcinomas to originate from interfollicular epidermis. Carcinogenesis. 2012;33:714-20 pubmed publisher
immunohistochemistry; rat	Agrawal A, Sharma M, Rai S, Singh B, Tiwari M, Chandra R. The effect of the aqueous extract of the roots of Asparagus racemosus on hepatocarcinogenesis initiated by diethylnitrosamine. Phytother Res. 2008;22:1175-82 pubmed publisher
immunohistochemistry - paraffin section; dogs	Zarfoss M, Klauss G, Newkirk K, Kiupel M, Jones Y, Colitz C, et al. Uveal spindle cell tumor of blue-eyed dogs: an immunohistochemical study. Vet Pathol. 2007;44:276-84 pubmed
western blot; mouse	Preyer M, Shu C, Wang J. Delayed activation of Bax by DNA damage in embryonic stem cells with knock-in mutations of the Abl nuclear localization signals. Cell Death Differ. 2007;14:1139-48 pubmed
western blot; human	Rosas Acosta G, Russell W, Deyrieux A, Russell D, Wilson V. A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers. Mol Cell Proteomics. 2005;4:56-72 pubmed
immunohistochemistry - paraffin section; rat; 1:25; fig 9	Mathur S, Kaur P, Sharma M, Katyal A, Singh B, Tiwari M, et al. The treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model. Phytomedicine. 2004;11:452-60 pubmed
western blot; mouse; 1:1000; fig 1	Zeng M, Narayanan L, Xu X, Prolla T, Liskay R, Glazer P. Ionizing radiation-induced apoptosis via separate Pms2- and p53-dependent pathways. Cancer Res. 2000;60:4889-93 pubmed
	Dong X, Kwan K. Yin Yang 1 is critical for mid-hindbrain neuroepithelium development and involved in cerebellar agenesis. Mol Brain. 2020;13:104 pubmed publisher
	Pollet M, Shaik S, Mescher M, Frauenstein K, Tigges J, Braun S, et al. The AHR represses nucleotide excision repair and apoptosis and contributes to UV-induced skin carcinogenesis. Cell Death Differ. 2018;25:1823-1836 pubmed publisher
	Sapio R, Nezdyur A, Krevetski M, Anikin L, Manna V, Minkovsky N, et al. Inhibition of post-transcriptional steps in ribosome biogenesis confers cytoprotection against chemotherapeutic agents in a p53-dependent manner. Sci Rep. 2017;7:9041 pubmed publisher
	Rousseau B, Jacquot C, Le Palabe J, Malleter M, Tomasoni C, Boutard T, et al. TP53 transcription factor for the NEDD9/HEF1/Cas-L gene: potential targets in Non-Small Cell Lung Cancer treatment. Sci Rep. 2015;5:10356 pubmed publisher

product information

Product Type :

Antibody

Product Name :

p53 Monoclonal Antibody (PAb 240)

Catalog # :

13-4100

Quantity :

100 µg

Price :

US 425.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1500

Species :

Human, Mouse, Rat

Clone :

PAb 240

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

Immunogen :

Recombinant p53 peptide.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1500

Aliases :

Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3

more info or order :

Invitrogen product webpage