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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
p53 Monoclonal Antibody (PAB1801)
catalog :
13-4000
quantity :
200µg
price :
US 345
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Pab1801
reactivity :
African green monkey, human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 14
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:2000; loading ...; fig 4c
Johnson R, Finger E, Olcina M, Vilalta M, Aguilera T, Miao Y, et al. Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow. Nat Cell Biol. 2016;18:1078-1089 pubmed publisher
  • western blot; human; fig 3
Martinez Zapien D, Ruiz F, Poirson J, Mitschler A, Ramirez J, Forster A, et al. Structure of the E6/E6AP/p53 complex required for HPV-mediated degradation of p53. Nature. 2016;529:541-5 pubmed publisher
  • western blot; human
Cao L, Ding J, Dong L, Zhao J, Su J, Wang L, et al. Negative Regulation of p21Waf1/Cip1 by Human INO80 Chromatin Remodeling Complex Is Implicated in Cell Cycle Phase G2/M Arrest and Abnormal Chromosome Stability. PLoS ONE. 2015;10:e0137411 pubmed publisher
  • western blot; human; fig 1
Patwardhan G, Hosain S, Liu D, Khiste S, Zhao Y, Bielawski J, et al. Ceramide modulates pre-mRNA splicing to restore the expression of wild-type tumor suppressor p53 in deletion-mutant cancer cells. Biochim Biophys Acta. 2014;1841:1571-80 pubmed publisher
  • western blot; human; 1:800
Williams R, Barnhill L, Kuo H, Lin W, Batova A, Yu A, et al. Chimeras of p14ARF and p16: functional hybrids with the ability to arrest growth. PLoS ONE. 2014;9:e88219 pubmed publisher
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:500
Hernández Bule M, Trillo M, Ubeda A. Molecular mechanisms underlying antiproliferative and differentiating responses of hepatocarcinoma cells to subthermal electric stimulation. PLoS ONE. 2014;9:e84636 pubmed publisher
  • western blot; human; 1:2000; fig 3
Frazzi R, Valli R, Tamagnini I, Casali B, Latruffe N, Merli F. Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation. Int J Cancer. 2013;132:1013-21 pubmed publisher
  • western blot; human; fig 1
Frazzi R, Casali B, Iori M, Nicoli D, Mammi C, Merli F. Increase in clusterin forms part of the stress response in Hodgkin's lymphoma. Int J Oncol. 2011;38:677-84 pubmed publisher
  • western blot; African green monkey; 1:2000; fig 3
  • western blot; human; 1:2000; fig 5
Kanezaki R, Toki T, Xu G, Narayanan R, Ito E. Cloning and characterization of the novel chimeric gene p53/FXR2 in the acute megakaryoblastic leukemia cell line CMK11-5. Tohoku J Exp Med. 2006;209:169-80 pubmed
  • immunohistochemistry - paraffin section; mouse; fig 1
Elso C, Lu X, Culiat C, Rutledge J, Cacheiro N, Generoso W, et al. Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice. Hum Mol Genet. 2004;13:2813-21 pubmed
  • immunohistochemistry - paraffin section; human
Barshack I, Goldberg I, Chowers Y, Weiss B, Horowitz A, Kopolovic J. Immunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue. J Clin Pathol. 2001;54:684-8 pubmed
  • western blot; human
Shenk J, Fisher C, Chen S, Zhou X, Tillman K, Shemshedini L. p53 represses androgen-induced transactivation of prostate-specific antigen by disrupting hAR amino- to carboxyl-terminal interaction. J Biol Chem. 2001;276:38472-9 pubmed
  • western blot; human
Evdokiou A, Atkins G, Bouralexis S, Hay S, Raggatt L, Cowled P, et al. Expression of alternatively-spliced MDM2 transcripts in giant cell tumours of bone. Int J Oncol. 2001;19:625-32 pubmed
Levy D, Rodríguez Buño R, Hu T, Moran A. The potential effects of tobacco control in China: projections from the China SimSmoke simulation model. BMJ. 2014;348:g1134 pubmed publisher
product information
Product Type :
Antibody
Product Name :
p53 Monoclonal Antibody (PAB1801)
Catalog # :
13-4000
Quantity :
200µg
Price :
US 345
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human
Applications :
Immunohistochemistry (Paraffin): 1:50-1:500, Immunoprecipitation: Assay Dependent, Western Blot: 1:1000
Species :
Human
Clone :
PAB1801
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.
Immunogen :
Recombinant p53 peptide.
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:50-1:500, Immunoprecipitation: Assay Dependent, Western Blot: 1:1000
Aliases :
antigen NY-CO-13, cellular tumor antigen p53, p53 tumor suppressor, phosphoprotein p53, transformation-related protein 53, TP53
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA