STAT5 alpha Monoclonal Antibody (ST5a-2H2) | Invitrogen 13-3600 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

STAT5 alpha Monoclonal Antibody (ST5a-2H2)

catalog :

13-3600

quantity :

100 µg

price :

375 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ST5a-2H2

reactivity :

African green monkey, human, mouse, rat, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

more info or order :

Invitrogen product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig s8	Hsieh W, Hsu T, Chang Y, Lai M. IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells. Nat Commun. 2018;9:463 pubmed publisher
immunohistochemistry - free floating section; rat; 1:100	MoravcovÃ¡ S, ÄŒervenÃ¡ K, PaÄesovÃ¡ D, BendovÃ¡ Z. Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide. J Neurosci Res. 2016;94:99-108 pubmed publisher
EMSA; bovine; fig 6	Nguyen M, Boutinaud M, PÃ©tridou B, Gabory A, Pannetier M, Chat S, et al. DNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking. PLoS ONE. 2014;9:e111556 pubmed publisher
western blot; human; fig 5	Elsarraj H, Hong Y, Valdez K, Carletti M, Salah S, Raimo M, et al. A novel role of microRNA146b in promoting mammary alveolar progenitor cell maintenance. J Cell Sci. 2013;126:2446-58 pubmed publisher
western blot; mouse; fig 5	Hoff P, Rakow A, Gaber T, Hahne M, Sentürk U, Strehl C, et al. Preoperative irradiation for the prevention of heterotopic ossification induces local inflammation in humans. Bone. 2013;55:93-101 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:3000; fig 3 western blot; mouse; 1:500; fig 4	Yip S, Eguchi R, Grattan D, Bunn S. Prolactin signalling in the mouse hypothalamus is primarily mediated by signal transducer and activator of transcription factor 5b but not 5a. J Neuroendocrinol. 2012;24:1484-91 pubmed publisher
western blot; human; fig 2	Barros P, Lam E, Jordan P, Matos P. Rac1 signalling modulates a STAT5/BCL-6 transcriptional switch on cell-cycle-associated target gene promoters. Nucleic Acids Res. 2012;40:7776-87 pubmed publisher
western blot; mouse; 1:1000; fig 7	Zhao H, Pearson E, Brooks D, Coon J, Chen D, Demura M, et al. A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice. Endocrinology. 2012;153:2701-13 pubmed publisher
chromatin immunoprecipitation; human; fig 6	Kabotyanski E, Huetter M, Xian W, Rijnkels M, Rosen J. Integration of prolactin and glucocorticoid signaling at the beta-casein promoter and enhancer by ordered recruitment of specific transcription factors and chromatin modifiers. Mol Endocrinol. 2006;20:2355-68 pubmed
immunoprecipitation; African green monkey; fig 4 western blot; African green monkey; fig 4	Aittomaki S, Pesu M, Groner B, Janne O, Palvimo J, Silvennoinen O. Cooperation among Stat1, glucocorticoid receptor, and PU.1 in transcriptional activation of the high-affinity Fc gamma receptor I in monocytes. J Immunol. 2000;164:5689-97 pubmed
	Ushiki T, Huntington N, Glaser S, Kiu H, Georgiou A, Zhang J, et al. Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells. PLoS ONE. 2016;11:e0162111 pubmed publisher
	Silver D, Naora H, Liu J, Cheng W, Montell D. Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility. Cancer Res. 2004;64:3550-8 pubmed
	Gewinner C, Hart G, Zachara N, Cole R, Beisenherz Huss C, Groner B. The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5. J Biol Chem. 2004;279:3563-72 pubmed
	Chong M, Cornish A, Darwiche R, Stanley E, Purton J, Godfrey D, et al. Suppressor of cytokine signaling-1 is a critical regulator of interleukin-7-dependent CD8+ T cell differentiation. Immunity. 2003;18:475-87 pubmed
	Saharinen P, Vihinen M, Silvennoinen O. Autoinhibition of Jak2 tyrosine kinase is dependent on specific regions in its pseudokinase domain. Mol Biol Cell. 2003;14:1448-59 pubmed
	Cornish A, Chong M, Davey G, Darwiche R, Nicola N, Hilton D, et al. Suppressor of cytokine signaling-1 regulates signaling in response to interleukin-2 and other gamma c-dependent cytokines in peripheral T cells. J Biol Chem. 2003;278:22755-61 pubmed
	Millot B, Montoliu L, Fontaine M, Mata T, Devinoy E. Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes. Biochem J. 2003;372:41-52 pubmed
	Xu M, Nie L, Kim S, Sun X. STAT5-induced Id-1 transcription involves recruitment of HDAC1 and deacetylation of C/EBPbeta. EMBO J. 2003;22:893-904 pubmed

product information

Product Type :

Antibody

Product Name :

STAT5 alpha Monoclonal Antibody (ST5a-2H2)

Catalog # :

13-3600

Quantity :

100 µg

Price :

375 USD

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 0.1-1 µg/mL, Gel Shift: 1-3 µg, Immunocytochemistry: 1-3 µg/mL, Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:50, Immunoprecipitation: 5 µg, Western Blot: 1-3 µg/mL

Species :

Human, Mouse, Rat

Clone :

ST5a-2H2

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

Immunogen :

A peptide corresponding to the C-terminus of murine Stat5a coupled to a protein carrier.

Format :

Liquid

Applications w/Dilutions :

Aliases :

AA959963; Mammary gland factor; mammary gland factor STAT5A; mammary gland factor; transcription factor; Mgf; Mpf; signal transducer and activator of transcription 5; signal transducer and activator of transcription 5a; signal transducer and activator of transcription factor 5A; STAT; STAT5; STAT5A; STAT5A, Mammary Gland Factor; STAT5A/MGF; STAT5B; STATA5

more info or order :

Invitrogen product webpage