product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
c-Myc Monoclonal Antibody (9E10)
catalog :
13-2500
quantity :
200 µg
price :
US 325.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
9E10
reactivity :
Trypanosoma brucei, human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, other
more info or order :
citations: 137
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2a
Biesemann A, Gorontzi A, Barr F, Gerke V. Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies. J Biol Chem. 2017;292:11631-11640 pubmed publisher
  • immunocytochemistry; Trypanosoma brucei; 1:50; loading ...; fig 3b
  • western blot; Trypanosoma brucei; 1:2000; loading ...; fig 3a
Harsman A, Oeljeklaus S, Wenger C, Huot J, Warscheid B, Schneider A. The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins. Nat Commun. 2016;7:13707 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 2a
Čajánek L, Glatter T, Nigg E. The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis. J Cell Sci. 2015;128:1674-82 pubmed publisher
  • western blot; human
Chandra S, Priyadarshini R, Madhavan V, Tikoo S, Hussain M, Mudgal R, et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci. 2013;126:3782-95 pubmed publisher
Gutierrez A, Kim J, Umbreit N, Asbury C, Davis T, Miller M, et al. Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments. Curr Biol. 2020;30:4491-4499.e5 pubmed publisher
Schnormeier A, Pommerenke C, Kaufmann M, Drexler H, Koeppel M. Genomic deregulation of PRMT5 supports growth and stress tolerance in chronic lymphocytic leukemia. Sci Rep. 2020;10:9775 pubmed publisher
Cottier S, Darwiche R, Meyenhofer F, Debelyy M, Schneiter R. The yeast cell wall protein Pry3 inhibits mating through highly conserved residues within the CAP domain. Biol Open. 2020;9: pubmed publisher
Gurkaslar H, Culfa E, Arslanhan M, Lince Faria M, Firat Karalar E. CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression. Cell Rep. 2020;31:107630 pubmed publisher
Manage K, Rogers A, Wallis D, Uebel C, Anderson D, Nguyen D, et al. A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans. elife. 2020;9: pubmed publisher
von Känel C, Muñoz Gómez S, Oeljeklaus S, Wenger C, Warscheid B, Wideman J, et al. Homologue replacement in the import motor of the mitochondrial inner membrane of trypanosomes. elife. 2020;9: pubmed publisher
Xie Y, Sun J, Han X, Turšić Wunder A, Toh J, Hong W, et al. Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization. Nat Commun. 2019;10:5078 pubmed publisher
Peng Z, Simeon R, Mitchell S, Zhang J, Feng H, Chen Z. Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027. mSphere. 2019;4: pubmed publisher
Ludlam W, Aoba T, Cuellar J, Bueno Carrasco M, Makaju A, Moody J, et al. Molecular architecture of the Bardet-Biedl syndrome protein 2-7-9 subcomplex. J Biol Chem. 2019;294:16385-16399 pubmed publisher
DeDiego M, Nogales A, Martinez Sobrido L, Topham D. Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication. MBio. 2019;10: pubmed publisher
Simeon R, Jiang M, Chamoun Emanuelli A, Yu H, Zhang Y, Meng R, et al. Selection and characterization of ultrahigh potency designed ankyrin repeat protein inhibitors of C. difficile toxin B. PLoS Biol. 2019;17:e3000311 pubmed publisher
Sigoillot M, Overtus M, Grodecka M, Scholl D, Garcia Pino A, Laeremans T, et al. Domain-interface dynamics of CFTR revealed by stabilizing nanobodies. Nat Commun. 2019;10:2636 pubmed publisher
Kim J, Jung K, Kim J, Kim S, Park H, Kim Y. Engineering of anti-human interleukin-4 receptor alpha antibodies with potent antagonistic activity. Sci Rep. 2019;9:7772 pubmed publisher
Kwak M, Kim H, Lkhamsuren K, Kim Y, Han M, Shin J, et al. Peroxiredoxin-mediated disulfide bond formation is required for nucleocytoplasmic translocation and secretion of HMGB1 in response to inflammatory stimuli. Redox Biol. 2019;24:101203 pubmed publisher
Venkatesan A, Fan J, Bouyain S, Price J. The Circadian tau Mutation in Casein Kinase 1 Is Part of a Larger Domain That Can Be Mutated to Shorten Circadian Period. Int J Mol Sci. 2019;20: pubmed publisher
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Orosa B, Yates G, Verma V, Srivastava A, Srivastava M, Campanaro A, et al. SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity. Nat Commun. 2018;9:5185 pubmed publisher
Park J, Kim Y, Yoo Y, Kim J, Jun S, Cho J, et al. Structural basis for arginine glycosylation of host substrates by bacterial effector proteins. Nat Commun. 2018;9:4283 pubmed publisher
Maksimov V, Oya E, Tanaka M, Kawaguchi T, Hachisuka A, Ekwall K, et al. The binding of Chp2's chromodomain to methylated H3K9 is essential for Chp2's role in heterochromatin assembly in fission yeast. PLoS ONE. 2018;13:e0201101 pubmed publisher
Longatti A, Schindler C, Collinson A, Jenkinson L, Matthews C, Fitzpatrick L, et al. High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles. Nanoscale. 2018;10:14230-14244 pubmed publisher
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Moore K, Logan M, Aldiri I, Roberts J, Steele M, Vetter M. C8orf46 homolog encodes a novel protein Vexin that is required for neurogenesis in Xenopus laevis. Dev Biol. 2018;437:27-40 pubmed publisher
Zhao Y, Wu X, Li X, Jiang L, Gui X, Liu Y, et al. TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function. Neuron. 2018;97:1023-1031.e7 pubmed publisher
Dauksaite V, Gotthardt M. Molecular basis of titin exon exclusion by RBM20 and the novel titin splice regulator PTB4. Nucleic Acids Res. 2018;46:5227-5238 pubmed publisher
Kim Y, Kwak M, Shin J, Hayuningtyas R, Choi J, Shin J. Inflachromene inhibits autophagy through modulation of Beclin 1 activity. J Cell Sci. 2018;131: pubmed publisher
Wood R, Ormsby A, Radwan M, Cox D, Sharma A, Vöpel T, et al. A biosensor-based framework to measure latent proteostasis capacity. Nat Commun. 2018;9:287 pubmed publisher
Karvonen H, Summala K, Niininen W, Barker H, Ungureanu D. Interaction between ROR1 and MuSK activation complex in myogenic cells. FEBS Lett. 2018;592:434-445 pubmed publisher
Käser S, Willemin M, Schnarwiler F, Schimanski B, Poveda Huertes D, Oeljeklaus S, et al. Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei. PLoS Pathog. 2017;13:e1006808 pubmed publisher
Kourtidis A, Necela B, Lin W, Lu R, Feathers R, Asmann Y, et al. Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling. J Cell Biol. 2017;216:3073-3085 pubmed publisher
Wenger C, Oeljeklaus S, Warscheid B, Schneider A, Harsman A. A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase. PLoS Pathog. 2017;13:e1006550 pubmed publisher
Kauke M, Traxlmayr M, Parker J, Kiefer J, Knihtila R, McGee J, et al. An engineered protein antagonist of K-Ras/B-Raf interaction. Sci Rep. 2017;7:5831 pubmed publisher
Zhang C, Lai M, Khandan L, Lee L, Chen Z, Junge H. Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function. Nat Commun. 2017;8:16050 pubmed publisher
Peikert C, Mani J, Morgenstern M, Käser S, Knapp B, Wenger C, et al. Charting organellar importomes by quantitative mass spectrometry. Nat Commun. 2017;8:15272 pubmed publisher
Rajgor D, Fiuza M, Parkinson G, Hanley J. The PICK1 Ca2+ sensor modulates N-methyl-d-aspartate (NMDA) receptor-dependent microRNA-mediated translational repression in neurons. J Biol Chem. 2017;292:9774-9786 pubmed publisher
Hubmacher D, Schneider M, Berardinelli S, Takeuchi H, Willard B, Reinhardt D, et al. Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease. Sci Rep. 2017;7:41871 pubmed publisher
Mani J, Rout S, Desy S, Schneider A. Mitochondrial protein import - Functional analysis of the highly diverged Tom22 orthologue of Trypanosoma brucei. Sci Rep. 2017;7:40738 pubmed publisher
Dubey R, Staker B, Foe I, Bogyo M, Myler P, Ngo H, et al. Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1. Mol Microbiol. 2017;103:618-634 pubmed publisher
Casamayor Genescà A, Pla A, Oliver Vila I, Pujals Fonts N, Marín Gallén S, Caminal M, et al. Clinical-scale expansion of CD34+ cord blood cells amplifies committed progenitors and rapid scid repopulation cells. N Biotechnol. 2017;35:19-29 pubmed publisher
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Käser S, Oeljeklaus S, Týc J, Vaughan S, Warscheid B, Schneider A. Outer membrane protein functions as integrator of protein import and DNA inheritance in mitochondria. Proc Natl Acad Sci U S A. 2016;113:E4467-75 pubmed publisher
Kanemori Y, Koga Y, Sudo M, Kang W, Kashiwabara S, Ikawa M, et al. Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse. Proc Natl Acad Sci U S A. 2016;113:E3696-705 pubmed publisher
Wang Y, Li Y, Hu G, Huang X, Rao H, Xiong X, et al. Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism. Cell Signal. 2016;28:1304-13 pubmed publisher
Boateng L, Bennin D, de Oliveira S, Huttenlocher A. Mammalian Actin-binding Protein-1/Hip-55 Interacts with FHL2 and Negatively Regulates Cell Invasion. J Biol Chem. 2016;291:13987-98 pubmed publisher
Guerrera D, Shah J, Vasileva E, Sluysmans S, Méan I, Jond L, et al. PLEKHA7 Recruits PDZD11 to Adherens Junctions to Stabilize Nectins. J Biol Chem. 2016;291:11016-29 pubmed publisher
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Hunt L, Xu B, Finkelstein D, Fan Y, Carroll P, Cheng P, et al. The glucose-sensing transcription factor MLX promotes myogenesis via myokine signaling. Genes Dev. 2015;29:2475-89 pubmed publisher
Hadzic E, Catillon M, Halavatyi A, Medves S, Van Troys M, Moes M, et al. Delineating the Tes Interaction Site in Zyxin and Studying Cellular Effects of Its Disruption. PLoS ONE. 2015;10:e0140511 pubmed publisher
Shi C, Huang X, Zhang B, Zhu D, Luo H, Lu Q, et al. The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein. PLoS ONE. 2015;10:e0138936 pubmed publisher
Asghar A, Lajeunesse A, Dulla K, Combes G, Thebault P, Nigg E, et al. Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation. Nat Commun. 2015;6:8364 pubmed publisher
Fan H, Dong W, Li Q, Zou X, Zhang Y, Wang J, et al. Ajuba Preferentially Binds LXRα/RXRγ Heterodimer to Enhance LXR Target Gene Expression in Liver Cells. Mol Endocrinol. 2015;29:1608-18 pubmed publisher
Li Q, Peng H, Fan H, Zou X, Liu Q, Zhang Y, et al. The LIM protein Ajuba promotes adipogenesis by enhancing PPARγ and p300/CBP interaction. Cell Death Differ. 2016;23:158-68 pubmed publisher
Merrikh C, Brewer B, Merrikh H. The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units. PLoS Genet. 2015;11:e1005289 pubmed publisher
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Desantis A, Bruno T, Catena V, De Nicola F, Goeman F, Iezzi S, et al. Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy. EMBO J. 2015;34:1214-30 pubmed publisher
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Shevchuk Z, Yurchenko M, Darekar S, Holodnuka Kholodnyuk I, Kashuba V, Kashuba E. Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells. Acta Naturae. 2013;5:85-9 pubmed
Wu S, Zhu W, Nhan T, Toth J, Petroski M, Wolf D. CAND1 controls in vivo dynamics of the cullin 1-RING ubiquitin ligase repertoire. Nat Commun. 2013;4:1642 pubmed publisher
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Huang Y, Chiang N, Hu C, Hsiao C, Cheng K, Tsai W, et al. Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains. Mol Cell Biol. 2012;32:1408-20 pubmed publisher
White D, Rafalska Metcalf I, Ivanov A, Corsinotti A, Peng H, Lee S, et al. The ATM substrate KAP1 controls DNA repair in heterochromatin: regulation by HP1 proteins and serine 473/824 phosphorylation. Mol Cancer Res. 2012;10:401-14 pubmed publisher
Challagundla K, Sun X, Zhang X, Devine T, Zhang Q, Sears R, et al. Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress. Mol Cell Biol. 2011;31:4007-21 pubmed publisher
Yu J, Deshmukh H, Payton J, Dunham C, Scheithauer B, Tihan T, et al. Array-based comparative genomic hybridization identifies CDK4 and FOXM1 alterations as independent predictors of survival in malignant peripheral nerve sheath tumor. Clin Cancer Res. 2011;17:1924-34 pubmed publisher
Soler Lopez M, Zanzoni A, Lluís R, Stelzl U, Aloy P. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res. 2011;21:364-76 pubmed publisher
Zanazzi G, Matthews G. Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development. Neural Dev. 2010;5:24 pubmed publisher
Dai M, Sun X, Lu H. Ribosomal protein L11 associates with c-Myc at 5 S rRNA and tRNA genes and regulates their expression. J Biol Chem. 2010;285:12587-94 pubmed publisher
Gwinn D, Asara J, Shaw R. Raptor is phosphorylated by cdc2 during mitosis. PLoS ONE. 2010;5:e9197 pubmed publisher
Koo B, Coe D, Dixon L, Somerville R, Nelson C, Wang L, et al. ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells. Am J Pathol. 2010;176:1494-504 pubmed publisher
Lim K, Brady D, Kashatus D, Ancrile B, Der C, Cox A, et al. Aurora-A phosphorylates, activates, and relocalizes the small GTPase RalA. Mol Cell Biol. 2010;30:508-23 pubmed publisher
Koo B, Park M, Jeon O, Kim D. Regulatory mechanism of matrix metalloprotease-2 enzymatic activity by factor Xa and thrombin. J Biol Chem. 2009;284:23375-85 pubmed publisher
Hood F, Royle S. Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis. J Cell Sci. 2009;122:2185-90 pubmed publisher
Huot M, Brown C, Lamarche Vane N, Richard S. An adaptor role for cytoplasmic Sam68 in modulating Src activity during cell polarization. Mol Cell Biol. 2009;29:1933-43 pubmed publisher
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Loewen C, Young B, Tavassoli S, Levine T. Inheritance of cortical ER in yeast is required for normal septin organization. J Cell Biol. 2007;179:467-83 pubmed
Itatsu K, Zen Y, Ohira S, Ishikawa A, Sato Y, Harada K, et al. Immunohistochemical analysis of the progression of flat and papillary preneoplastic lesions in intrahepatic cholangiocarcinogenesis in hepatolithiasis. Liver Int. 2007;27:1174-84 pubmed
van Buul J, Allingham M, Samson T, Meller J, Boulter E, Garcia Mata R, et al. RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration. J Cell Biol. 2007;178:1279-93 pubmed
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Koo B, Longpre J, Somerville R, Alexander J, Leduc R, Apte S. Regulation of ADAMTS9 secretion and enzymatic activity by its propeptide. J Biol Chem. 2007;282:16146-54 pubmed
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Wagner W, Fodor E, Ginsburg A, Hammer J. The binding of DYNLL2 to myosin Va requires alternatively spliced exon B and stabilizes a portion of the myosin's coiled-coil domain. Biochemistry. 2006;45:11564-77 pubmed
O Donnell K, Yu D, Zeller K, Kim J, Racke F, Thomas Tikhonenko A, et al. Activation of transferrin receptor 1 by c-Myc enhances cellular proliferation and tumorigenesis. Mol Cell Biol. 2006;26:2373-86 pubmed
Spence H, McGarry L, Chew C, Carragher N, Scott Carragher L, Yuan Z, et al. AP-1 differentially expressed proteins Krp1 and fibronectin cooperatively enhance Rho-ROCK-independent mesenchymal invasion by altering the function, localization, and activity of nondifferentially expressed proteins. Mol Cell Biol. 2006;26:1480-95 pubmed
Iwata A, Riley B, Johnston J, Kopito R. HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin. J Biol Chem. 2005;280:40282-92 pubmed
Aqeilan R, Donati V, Palamarchuk A, Trapasso F, Kaou M, Pekarsky Y, et al. WW domain-containing proteins, WWOX and YAP, compete for interaction with ErbB-4 and modulate its transcriptional function. Cancer Res. 2005;65:6764-72 pubmed
Jansen S, Stefan C, Creemers J, Waelkens E, Van Eynde A, Stalmans W, et al. Proteolytic maturation and activation of autotaxin (NPP2), a secreted metastasis-enhancing lysophospholipase D. J Cell Sci. 2005;118:3081-9 pubmed
Palamarchuk A, Efanov A, Maximov V, Aqeilan R, Croce C, Pekarsky Y. Akt phosphorylates Tal1 oncoprotein and inhibits its repressor activity. Cancer Res. 2005;65:4515-9 pubmed
Liewen H, Meinhold Heerlein I, Oliveira V, Schwarzenbacher R, Luo G, Wadle A, et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp Cell Res. 2005;306:24-34 pubmed
Torres V, Ivie S, McClain M, Cover T. Functional properties of the p33 and p55 domains of the Helicobacter pylori vacuolating cytotoxin. J Biol Chem. 2005;280:21107-14 pubmed
Honda A, Al Awar O, Hay J, Donaldson J. Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE. J Cell Biol. 2005;168:1039-51 pubmed
Goldstein A, Jan Y, Luo L. Function and regulation of Tumbleweed (RacGAP50C) in neuroblast proliferation and neuronal morphogenesis. Proc Natl Acad Sci U S A. 2005;102:3834-9 pubmed
Mo Y, Yu Y, Theodosiou E, Ee P, Beck W. A role for Ubc9 in tumorigenesis. Oncogene. 2005;24:2677-83 pubmed
Soosairajah J, Maiti S, Wiggan O, Sarmiere P, Moussi N, Sarcevic B, et al. Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin. EMBO J. 2005;24:473-86 pubmed
Zhang J, Wang Y, Zhou Y, Cao Z, Huang P, Lu B. Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. FEBS Lett. 2005;579:559-66 pubmed
Sauvonnet N, Dujeancourt A, Dautry Varsat A. Cortactin and dynamin are required for the clathrin-independent endocytosis of gammac cytokine receptor. J Cell Biol. 2005;168:155-63 pubmed
Pyagay P, Heroult M, Wang Q, Lehnert W, Belden J, Liaw L, et al. Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration. Circ Res. 2005;96:261-8 pubmed
Sakamoto K, Chao W, Katsube K, Yamaguchi A. Distinct roles of EGF repeats for the Notch signaling system. Exp Cell Res. 2005;302:281-91 pubmed
Cardinale A, Filesi I, Vetrugno V, Pocchiari M, Sy M, Biocca S. Trapping prion protein in the endoplasmic reticulum impairs PrPC maturation and prevents PrPSc accumulation. J Biol Chem. 2005;280:685-94 pubmed
Gao L, Macara I. Isoforms of the polarity protein par6 have distinct functions. J Biol Chem. 2004;279:41557-62 pubmed
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product information
Product Type :
Antibody
Product Name :
c-Myc Monoclonal Antibody (9E10)
Catalog # :
13-2500
Quantity :
200 µg
Price :
US 325.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
ELISA: 0.1-1.0 µg/mL, Gel Shift: Assay-dependent, Immunocytochemistry: 2-10 µg/mL, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: 2-5 µg, Western Blot: 0.5-2 µg/mL
Species :
Human, Mouse
Clone :
9E10
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.
Immunogen :
A 32 amino acid synthetic peptide (aa 408-439) derived from the C-terminus of the human c-myc protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1.0 µg/mL, Gel Shift: Assay-dependent, Immunocytochemistry: 2-10 µg/mL, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: 2-5 µg, Western Blot: 0.5-2 µg/mL
Aliases :
AU016757; Avian myelocytomatosis viral (v-myc) oncogene homolog; avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; class E basic helix-loop-helix protein 39; cmyc; c-Myc; c-myc epitope tag; c-myc proto-oncogene; mMyc; MRTL; Myc; Myc protein; Myc proto-oncogene protein; MYC proto-oncogene, bHLH transcription factor; Myc2; MYCC; myc-related translation/localization regulatory factor; myelocytomatosis oncogene; myelocytomatosis viral oncogene homolog; Niard; Nird; Proto-oncogene c-Myc; RNCMYC; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene-like protein
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA