N-cadherin Monoclonal Antibody (NCD-2) | Invitrogen 13-2100 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

N-cadherin Monoclonal Antibody (NCD-2)

catalog :

13-2100

quantity :

100 µg

price :

US 459

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

NCD-2

reactivity :

hamsters, Japanese quail, human, mouse, chicken, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; chicken; 1:200; fig 2 western blot; chicken; 1:2000; fig 5	Herrera A, Saade M, Menendez A, Marti E, Pons S. Sustained Wnt/?-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole. Nat Commun. 2014;5:4168 pubmed publisher
western blot; human; fig 1 immunocytochemistry; chicken; fig 2 western blot; hamsters; fig 1	BRUSES J. Cell surface localization of ?3?4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility. PLoS ONE. 2013;8:e62435 pubmed publisher
blocking or activating experiments; Japanese quail	Romeih M, Cakstina I, Zile M. Retinoic acid is a negative physiological regulator of N-cadherin during early avian heart morphogenesis. Dev Growth Differ. 2009;51:753-67 pubmed publisher
immunohistochemistry; chicken; fig 5	Marrs G, Theisen C, BRUSES J. N-cadherin modulates voltage activated calcium influx via RhoA, p120-catenin, and myosin-actin interaction. Mol Cell Neurosci. 2009;40:390-400 pubmed publisher
immunohistochemistry - paraffin section; chicken; 5 ug/ml; fig 1	Warchol M. Characterization of supporting cell phenotype in the avian inner ear: implications for sensory regeneration. Hear Res. 2007;227:11-8 pubmed
immunocytochemistry; mouse	Foty R, Steinberg M. The differential adhesion hypothesis: a direct evaluation. Dev Biol. 2005;278:255-63 pubmed
flow cytometry; chicken	Duguay D, Foty R, Steinberg M. Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants. Dev Biol. 2003;253:309-23 pubmed
immunohistochemistry; mouse	Mahlapuu M, Ormestad M, Enerback S, Carlsson P. The forkhead transcription factor Foxf1 is required for differentiation of extra-embryonic and lateral plate mesoderm. Development. 2001;128:155-66 pubmed
	He Z, Lei S, Liang F, Tan L, Zhang W, Xie L, et al. Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation. J Oncol. 2021;2021:4475192 pubmed publisher
	Herrera A, Menendez A, Torroba B, Ochoa A, Pons S. Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity. J Cell Biol. 2021;220: pubmed publisher
	Liu J, Rao Y, Cheung M, Hui M, Wu M, Chan L, et al. Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration. Nat Commun. 2017;8:1185 pubmed publisher
	Theveneau E, Marchant L, Kuriyama S, Gull M, Moepps B, Parsons M, et al. Collective chemotaxis requires contact-dependent cell polarity. Dev Cell. 2010;19:39-53 pubmed publisher
	Ciesiolka M, Delvaeye M, Van Imschoot G, Verschuere V, McCrea P, van Roy F, et al. p120 catenin is required for morphogenetic movements involved in the formation of the eyes and the craniofacial skeleton in Xenopus. J Cell Sci. 2004;117:4325-39 pubmed
	Robinson E, Zazzali K, Corbett S, Foty R. Alpha5beta1 integrin mediates strong tissue cohesion. J Cell Sci. 2003;116:377-86 pubmed
	Ryan P, Foty R, Kohn J, Steinberg M. Tissue spreading on implantable substrates is a competitive outcome of cell-cell vs. cell-substratum adhesivity. Proc Natl Acad Sci U S A. 2001;98:4323-7 pubmed
	Hirano S, Kimoto N, Shimoyama Y, Hirohashi S, Takeichi M. Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization. Cell. 1992;70:293-301 pubmed

product information

Product Type :

Antibody

Product Name :

N-cadherin Monoclonal Antibody (NCD-2)

Catalog # :

13-2100

Quantity :

100 µg

Price :

US 459

Clonality :

Monoclonal

Purity :

purified

Host :

Rat

Reactivity :

Chicken, Xenopus laevis

Applications :

Immunofluorescence: Assay Dependent, Immunohistochemistry (Frozen): 10 µg/mL, Inhibition Assays: 100 µg/mL, Western Blot: 1-10 µg/mL

Species :

Chicken, Xenopus laevis

Clone :

NCD-2

Isotype :

IgG2a

Storage :

4° C

Description :

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

Immunogen :

Neuronal tissue of chick embryos.

Format :

Lyophilized

Applications w/Dilutions :

Immunofluorescence: Assay Dependent, Immunohistochemistry (Frozen): 10 µg/mL, Inhibition Assays: 100 µg/mL, Western Blot: 1-10 µg/mL

Aliases :

bib; cadherin 2; cadherin 2 L homeolog; cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, neuronal; cadherin 2, type 1; cadherin 2, type 1, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal) L homeolog; cadherin 2, type 1, N-cadherin a (neuronal); Cadherin2; cadherin-2; Cadherin-2A; Cadherin-N; calcium-dependent adhesion protein, neuronal; CD325; cdh2; cdh2.L; cdh2a; cdh2-a; cdh2-b; CDHN; cdhn-a; CDw325; CDw325 antigen; cell adhesion molecule; glass onion; glo; I79_014914; labyrinth; LOC100344571; lyr; NCAD; N-Cad; N-cadherin; N-cadherin 1; N-cadherin A; N-cadherin precursor; N-cadherin-2; Neural cadherin; neural cadherin A; Neural cadherin-2; neural-cadherin; pac; Parachute; wu:fb47h04; XELAEV_18031953mg; ZNCAD

more info or order :

Invitrogen product webpage