NEFM Monoclonal Antibody (RMO-270) | Invitrogen 13-0700 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NEFM Monoclonal Antibody (RMO-270)

catalog :

13-0700

quantity :

200 µg

price :

425 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RMO-270

reactivity :

human, mouse, rat, chicken, zebrafish , Xenopus laevis

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 52

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:1000; loading ...; tbl 1	Pasquini J, Barrantes F, QuintÃ¡ H. Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1. J Comp Neurol. 2017;525:2861-2875 pubmed publisher
immunohistochemistry; chicken; 1:1000; fig 1f	Faunes M, Botelho J, Wild J. Innervation of the syrinx of the zebra finch (Taeniopygia guttata). J Comp Neurol. 2017;525:2847-2860 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1.500; loading ...; fig s6u	Himmels P, Paredes I, Adler H, Karakatsani A, Luck R, Marti H, et al. Motor neurons control blood vessel patterning in the developing spinal cord. Nat Commun. 2017;8:14583 pubmed publisher
immunohistochemistry - paraffin section; chicken; 1:1000; loading ...; fig 5c	Ware M, Hamdi Rozé H, Le Friec J, David V, Dupé V. Regulation of downstream neuronal genes by proneural transcription factors during initial neurogenesis in the vertebrate brain. Neural Dev. 2016;11:22 pubmed
immunohistochemistry; Xenopus laevis; 1:500; loading ...; fig 1c	Gambrill A, Faulkner R, Cline H. Experience-dependent plasticity of excitatory and inhibitory intertectal inputs in Xenopus tadpoles. J Neurophysiol. 2016;116:2281-2297 pubmed publisher
flow cytometry; chicken; 1:5000; fig 1 immunohistochemistry; chicken; 1:5000; fig s1	Patthey C, Clifford H, Haerty W, Ponting C, Shimeld S, Begbie J. Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick. Neural Dev. 2016;11:3 pubmed publisher
immunohistochemistry; chicken; 1:5000	Smith A, Fleenor S, Begbie J. Changes in gene expression and cell shape characterise stages of epibranchial placode-derived neuron maturation in the chick. J Anat. 2015;227:89-102 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:250; fig 4j-l, m-o	Gay M, Valenta T, Herr P, Paratore Hari L, Basler K, Sommer L. Distinct adhesion-independent functions of Î²-catenin control stage-specific sensory neurogenesis and proliferation. BMC Biol. 2015;13:24 pubmed publisher
immunocytochemistry; rat	Jung Y, Ng J, Keating C, Senthil Kumar P, Zhao J, Randolph M, et al. Comprehensive evaluation of peripheral nerve regeneration in the acute healing phase using tissue clearing and optical microscopy in a rodent model. PLoS ONE. 2014;9:e94054 pubmed publisher
immunohistochemistry; chicken	Lours Calet C, Alvares L, El Hanfy A, Gandesha S, Walters E, Sobreira D, et al. Evolutionarily conserved morphogenetic movements at the vertebrate head-trunk interface coordinate the transport and assembly of hypopharyngeal structures. Dev Biol. 2014;390:231-46 pubmed publisher
immunohistochemistry; chicken; 1:200	Ma Z, Wang G, Cheng X, Chuai M, Kurihara H, Lee K, et al. Excess caffeine exposure impairs eye development during chick embryogenesis. J Cell Mol Med. 2014;18:1134-43 pubmed publisher
immunohistochemistry; chicken; 1:5000; fig 1	Freter S, Fleenor S, Freter R, Liu K, Begbie J. Cranial neural crest cells form corridors prefiguring sensory neuroblast migration. Development. 2013;140:3595-600 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 7	Yoo M, Khaled M, Gibbs K, Kim J, Kowalewski B, Dierks T, et al. Arylsulfatase B improves locomotor function after mouse spinal cord injury. PLoS ONE. 2013;8:e57415 pubmed publisher
immunohistochemistry - frozen section; chicken; 1:50	Sanyas I, Bozon M, Moret F, Castellani V. Motoneuronal Sema3C is essential for setting stereotyped motor tract positioning in limb-derived chemotropic semaphorins. Development. 2012;139:3633-43 pubmed publisher
immunohistochemistry; mouse; 1:3000; fig 2	Simrick S, Lickert H, Basson M. Sprouty genes are essential for the normal development of epibranchial ganglia in the mouse embryo. Dev Biol. 2011;358:147-55 pubmed publisher
immunocytochemistry; mouse; 1:1500; fig 7	Niederkofler V, Baeriswyl T, Ott R, Stoeckli E. Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance. Development. 2010;137:427-35 pubmed publisher
immunohistochemistry; human; 5 ug/ml; fig 3	Arthur A, Shi S, Zannettino A, Fujii N, Gronthos S, Koblar S. Implanted adult human dental pulp stem cells induce endogenous axon guidance. Stem Cells. 2009;27:2229-37 pubmed publisher
immunocytochemistry; human; 1:100; fig 3	Colakoglu G, Brown A. Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing. J Cell Biol. 2009;185:769-77 pubmed publisher
immunohistochemistry; chicken; 1:100; fig 3B	Moret F, Renaudot C, Bozon M, Castellani V. Semaphorin and neuropilin co-expression in motoneurons sets axon sensitivity to environmental semaphorin sources during motor axon pathfinding. Development. 2007;134:4491-501 pubmed
immunohistochemistry - paraffin section; chicken; 1:10,000	Graham A, Blentic A, Duque S, Begbie J. Delamination of cells from neurogenic placodes does not involve an epithelial-to-mesenchymal transition. Development. 2007;134:4141-5 pubmed
immunohistochemistry; mouse; fig 2 immunohistochemistry; chicken; fig 2	Borday C, Coutinho A, Germon I, Champagnat J, Fortin G. Pre-/post-otic rhombomeric interactions control the emergence of a fetal-like respiratory rhythm in the mouse embryo. J Neurobiol. 2006;66:1285-301 pubmed
immunohistochemistry - paraffin section; chicken; 1:10,000	Ferguson C, Graham A. Redefining the head-trunk interface for the neural crest. Dev Biol. 2004;269:70-80 pubmed
immunohistochemistry; mouse	Polo Parada L, Bose C, Plattner F, Landmesser L. Distinct roles of different neural cell adhesion molecule (NCAM) isoforms in synaptic maturation revealed by analysis of NCAM 180 kDa isoform-deficient mice. J Neurosci. 2004;24:1852-64 pubmed
immunohistochemistry; zebrafish ; 1:10000	Walshe J, Mason I. Fgf signalling is required for formation of cartilage in the head. Dev Biol. 2003;264:522-36 pubmed
western blot; mouse; 1:1000; fig 1	Dashiell S, Tanner S, Pant H, Quarles R. Myelin-associated glycoprotein modulates expression and phosphorylation of neuronal cytoskeletal elements and their associated kinases. J Neurochem. 2002;81:1263-72 pubmed
immunohistochemistry; chicken; 1:500; fig 1	Golding J, Dixon M, Gassmann M. Cues from neuroepithelium and surface ectoderm maintain neural crest-free regions within cranial mesenchyme of the developing chick. Development. 2002;129:1095-105 pubmed
immunohistochemistry; chicken	Mootoosamy R, Dietrich S. Distinct regulatory cascades for head and trunk myogenesis. Development. 2002;129:573-83 pubmed
immunohistochemistry; chicken; 1:500; fig 2	Britto J, Tannahill D, Keynes R. A critical role for sonic hedgehog signaling in the early expansion of the developing brain. Nat Neurosci. 2002;5:103-10 pubmed
immunohistochemistry; chicken; 1:10000; fig 1	Hunter E, Begbie J, Mason I, Graham A. Early development of the mesencephalic trigeminal nucleus. Dev Dyn. 2001;222:484-93 pubmed
immunohistochemistry - paraffin section; chicken; fig 5	Hashino E, Johnson E, Milbrandt J, Shero M, Salvi R, Cohan C. Multiple actions of neurturin correlate with spatiotemporal patterns of Ret expression in developing chick cranial ganglion neurons. J Neurosci. 1999;19:8476-86 pubmed
immunohistochemistry; chicken	Jungbluth S, Bell E, Lumsden A. Specification of distinct motor neuron identities by the singular activities of individual Hox genes. Development. 1999;126:2751-8 pubmed
immunohistochemistry; chicken; 1:10000	MacKenzie S, Walsh F, Graham A. Migration of hypoglossal myoblast precursors. Dev Dyn. 1998;213:349-58 pubmed
immunohistochemistry; chicken; 1:2000	Logan C, Wingate R, McKay I, Lumsden A. Tlx-1 and Tlx-3 homeobox gene expression in cranial sensory ganglia and hindbrain of the chick embryo: markers of patterned connectivity. J Neurosci. 1998;18:5389-402 pubmed
western blot; rat	Veeranna -, Amin N, Ahn N, Jaffe H, Winters C, Grant P, et al. Mitogen-activated protein kinases (Erk1,2) phosphorylate Lys-Ser-Pro (KSP) repeats in neurofilament proteins NF-H and NF-M. J Neurosci. 1998;18:4008-21 pubmed
immunohistochemistry; Xenopus laevis; fig 2	Blanco R, Orkand P. Astrocytes and regenerating axons at the proximal stump of the severed frog optic nerve. Cell Tissue Res. 1996;286:337-45 pubmed
	Si Z, Wang G, Han S, Jin Y, Hu Y, He M, et al. CNTF and Nrf2 Are Coordinately Involved in Regulating Self-Renewal and Differentiation of Neural Stem Cell during Embryonic Development. iScience. 2019;19:303-315 pubmed publisher
	Manogaran P, Samardzija M, Schad A, Wicki C, Walker Egger C, Rudin M, et al. Retinal pathology in experimental optic neuritis is characterized by retrograde degeneration and gliosis. Acta Neuropathol Commun. 2019;7:116 pubmed publisher
	Walker C, Uchida A, Li Y, Trivedi N, Fenn J, Monsma P, et al. Local Acceleration of Neurofilament Transport at Nodes of Ranvier. J Neurosci. 2019;39:663-677 pubmed publisher
	Tymanskyj S, Yang B, Verhey K, Ma L. MAP7 regulates axon morphogenesis by recruiting kinesin-1 to microtubules and modulating organelle transport. elife. 2018;7: pubmed publisher
	Ito N, Katoh K, Kushige H, Saito Y, Umemoto T, Matsuzaki Y, et al. Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency. Sci Rep. 2018;8:1634 pubmed publisher
	Nogueira J, Hawrot K, Sharpe C, Noble A, Wood W, Jorge E, et al. The emergence of Pax7-expressing muscle stem cells during vertebrate head muscle development. Front Aging Neurosci. 2015;7:62 pubmed publisher
	SCANLON C, Banerjee R, Inglehart R, Liu M, Russo N, Hariharan A, et al. Galanin modulates the neural niche to favour perineural invasion in head and neck cancer. Nat Commun. 2015;6:6885 pubmed publisher
	Westenskow P, McKean J, Kubo F, Nakagawa S, Fuhrmann S. Ectopic Mitf in the embryonic chick retina by co-transfection of ?-catenin and Otx2. Invest Ophthalmol Vis Sci. 2010;51:5328-35 pubmed publisher
	Arthur A, Rychkov G, Shi S, Koblar S, Gronthos S. Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues. Stem Cells. 2008;26:1787-95 pubmed publisher
	Stepanek L, Stoker A, Stoeckli E, Bixby J. Receptor tyrosine phosphatases guide vertebrate motor axons during development. J Neurosci. 2005;25:3813-23 pubmed
	Kubo F, Takeichi M, Nakagawa S. Wnt2b controls retinal cell differentiation at the ciliary marginal zone. Development. 2003;130:587-98 pubmed
	Webber C, Hocking J, Yong V, Stange C, McFarlane S. Metalloproteases and guidance of retinal axons in the developing visual system. J Neurosci. 2002;22:8091-100 pubmed
	Kim O, Ariano M, Lazzarini R, Levine M, Sibley D. Neurofilament-M interacts with the D1 dopamine receptor to regulate cell surface expression and desensitization. J Neurosci. 2002;22:5920-30 pubmed
	Fischer A, Dierks B, Reh T. Exogenous growth factors induce the production of ganglion cells at the retinal margin. Development. 2002;129:2283-91 pubmed
	Charter N, Mahal L, Koshland D, Bertozzi C. Differential effects of unnatural sialic acids on the polysialylation of the neural cell adhesion molecule and neuronal behavior. J Biol Chem. 2002;277:9255-61 pubmed
	Larsen C, Zeltser L, Lumsden A. Boundary formation and compartition in the avian diencephalon. J Neurosci. 2001;21:4699-711 pubmed
	Bell E, Wingate R, Lumsden A. Homeotic transformation of rhombomere identity after localized Hoxb1 misexpression. Science. 1999;284:2168-71 pubmed

product information

Product Type :

Antibody

Product Name :

NEFM Monoclonal Antibody (RMO-270)

Catalog # :

13-0700

Quantity :

200 µg

Price :

425 USD

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 0.1-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): Assay Dependent, Immunohistochemistry: 5-10 µg/mL, Immunoprecipitation: 2-5 µg, Western Blot: 0.5-1 µg/mL

Species :

Human, Mouse, Rat

Clone :

RMO-270

Isotype :

IgG2a, kappa

Storage :

-20°C

Description :

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Immunogen :

Rat neurofilaments

Format :

Liquid

Applications w/Dilutions :

Aliases :

160 kDa neurofilament protein; major neuronal intermediate filament subunit; middle molecular weight neurofilament protein NF-M(2); nef3; NEFM; nef-m; nefm.S; nefm-a; nefm-b; neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; neurofilament-M subunit; neuronal intermediate filament; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg

more info or order :

Invitrogen product webpage