Zap-70 Monoclonal Antibody (ZAP-70-6F7) | Invitrogen 13-0600 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Zap-70 Monoclonal Antibody (ZAP-70-6F7)

catalog :

13-0600

quantity :

100 µg

price :

425 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ZAP-70-6F7

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; mouse; fig 3	Demetriou M, Granovsky M, Quaggin S, Dennis J. Negative regulation of T-cell activation and autoimmunity by Mgat5 N-glycosylation. Nature. 2001;409:733-9 pubmed
	Alonso A, Bottini N, Bruckner S, Rahmouni S, Williams S, Schoenberger S, et al. Lck dephosphorylation at Tyr-394 and inhibition of T cell antigen receptor signaling by Yersinia phosphatase YopH. J Biol Chem. 2004;279:4922-8 pubmed
	Bottini N, Stefanini L, Williams S, Alonso A, Jascur T, Abraham R, et al. Activation of ZAP-70 through specific dephosphorylation at the inhibitory Tyr-292 by the low molecular weight phosphotyrosine phosphatase (LMPTP). J Biol Chem. 2002;277:24220-4 pubmed

product information

Product Type :

Antibody

Product Name :

Zap-70 Monoclonal Antibody (ZAP-70-6F7)

Catalog # :

13-0600

Quantity :

100 µg

Price :

425 USD

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ELISA: Assay Dependent, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunoprecipitation: Assay Dependent, Western Blot: 1-3 µg/mL

Species :

Human, Mouse

Clone :

ZAP-70-6F7

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Immunogen :

Peptide derived from a.a. 326-341 of human ZAP-70.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay Dependent, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunoprecipitation: Assay Dependent, Western Blot: 1-3 µg/mL

Aliases :

70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Srk; STCD; STD; syk-related protein tyrosine kinase; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; ZA70; Zap70; ZAP-70; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

more info or order :

Invitrogen product webpage