NEFM Monoclonal Antibody (RMO-44) | Invitrogen 13-0500 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NEFM Monoclonal Antibody (RMO-44)

catalog :

13-0500

quantity :

200 µg

price :

425 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RMO-44

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; loading ...	Cudré Cung H, Zavadakova P, Do Vale Pereira S, Remacle N, Henry H, Ivanisevic J, et al. Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria. Mol Genet Metab. 2016;119:57-67 pubmed publisher
immunohistochemistry; zebrafish ; 1:100	Shah A, Davey C, Whitebirch A, Miller A, Moens C. Rapid reverse genetic screening using CRISPR in zebrafish. Nat Methods. 2015;12:535-40 pubmed publisher
immunohistochemistry; zebrafish ; 1:50	Campbell P, Shen K, Sapio M, Glenn T, Talbot W, Marlow F. Unique function of Kinesin Kif5A in localization of mitochondria in axons. J Neurosci. 2014;34:14717-32 pubmed publisher
western blot; mouse; fig 1	Rao M, Yuan A, Campbell J, Kumar A, Nixon R. The C-terminal domains of NF-H and NF-M subunits maintain axonal neurofilament content by blocking turnover of the stationary neurofilament network. PLoS ONE. 2012;7:e44320 pubmed publisher
western blot; mouse; fig 1	Kovacs K, Lajtha A, Sershen H. Effect of nicotine and cocaine on neurofilaments and receptors in whole brain tissue and synaptoneurosome preparations. Brain Res Bull. 2010;82:109-17 pubmed publisher
immunohistochemistry; zebrafish ; 1:50	Lowery L, De Rienzo G, Gutzman J, Sive H. Characterization and classification of zebrafish brain morphology mutants. Anat Rec (Hoboken). 2009;292:94-106 pubmed publisher
immunohistochemistry - frozen section; rat; fig 1	Braissant O, Cagnon L, Monnet Tschudi F, Speer O, Wallimann T, Honegger P, et al. Ammonium alters creatine transport and synthesis in a 3D culture of developing brain cells, resulting in secondary cerebral creatine deficiency. Eur J Neurosci. 2008;27:1673-85 pubmed publisher
immunohistochemistry; zebrafish ; 1:50; fig 7	Lowery L, Rubin J, Sive H. Whitesnake/sfpq is required for cell survival and neuronal development in the zebrafish. Dev Dyn. 2007;236:1347-57 pubmed
immunohistochemistry; zebrafish ; 1:50	Wiellette E, Sive H. vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain. Development. 2003;130:3821-9 pubmed
immunohistochemistry - frozen section; rat	Braissant O, Henry H, Villard A, Zurich M, Loup M, Eilers B, et al. Ammonium-induced impairment of axonal growth is prevented through glial creatine. J Neurosci. 2002;22:9810-20 pubmed
immunohistochemistry - paraffin section; human; 1:1000; fig 3	Katayama S, Watanabe C, Noda K, Ohishi H, Yamamura Y, Nishisaka T, et al. Numerous conglomerate inclusions in slowly progressive familial amyotrophic lateral sclerosis with posterior column involvement. J Neurol Sci. 1999;171:72-7 pubmed
western blot; rat; fig 2d	Bush M, Miyashiro J, Ingram V. Activation of a neurofilament kinase, a tau kinase, and a tau phosphatase by decreased ATP levels in nerve growth factor-differentiated PC-12 cells. Proc Natl Acad Sci U S A. 1995;92:1861-5 pubmed
	Maurer J, SAGERSTROM C. A parental requirement for dual-specificity phosphatase 6 in zebrafish. BMC Dev Biol. 2018;18:6 pubmed publisher
	Marsh A, Michel J, Adke A, Heckman E, Miller A. Asymmetry of an Intracellular Scaffold at Vertebrate Electrical Synapses. Curr Biol. 2017;27:3561-3567.e4 pubmed publisher
	Miller A, Whitebirch A, Shah A, Marsden K, Granato M, O BRIEN J, et al. A genetic basis for molecular asymmetry at vertebrate electrical synapses. elife. 2017;6: pubmed publisher
	Henderson M, Chung C, Riddle D, Zhang B, Gathagan R, Seeholzer S, et al. Unbiased Proteomics of Early Lewy Body Formation Model Implicates Active Microtubule Affinity-Regulating Kinases (MARKs) in Synucleinopathies. J Neurosci. 2017;37:5870-5884 pubmed publisher
	Lecaudey V, Anselme I, Rosa F, Schneider Maunoury S. The zebrafish Iroquois gene iro7 positions the r4/r5 boundary and controls neurogenesis in the rostral hindbrain. Development. 2004;131:3121-31 pubmed
	Xia C, Roberts E, Her L, Liu X, Williams D, Cleveland D, et al. Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A. J Cell Biol. 2003;161:55-66 pubmed
	Maves L, Jackman W, Kimmel C. FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain. Development. 2002;129:3825-37 pubmed

product information

Product Type :

Antibody

Product Name :

NEFM Monoclonal Antibody (RMO-44)

Catalog # :

13-0500

Quantity :

200 µg

Price :

425 USD

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Leech, Rat

Applications :

Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry (Paraffin): 1:50, Immunohistochemistry: 5-10 µg/mL, Immunoprecipitation: 2-5 µg, Western Blot: Assay Dependent

Species :

Human, Leech, Rat

Clone :

RMO-44

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Immunogen :

Rat neurofilaments

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry (Paraffin): 1:50, Immunohistochemistry: 5-10 µg/mL, Immunoprecipitation: 2-5 µg, Western Blot: Assay Dependent

Aliases :

160 kDa neurofilament protein; major neuronal intermediate filament subunit; middle molecular weight neurofilament protein NF-M(2); nef3; NEFM; nef-m; nefm.S; nefm-a; nefm-b; neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; neurofilament-M subunit; neuronal intermediate filament; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg

more info or order :

Invitrogen product webpage