NEFL Monoclonal Antibody (DA2) | Invitrogen 13-0400 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NEFL Monoclonal Antibody (DA2)

catalog :

13-0400

quantity :

200 µg

price :

425 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DA2

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; fig 5	Liu Q, Zhang R, Li D, Cheng S, Yang Y, Tian T, et al. Muse Cells, a New Type of Pluripotent Stem Cell Derived from Human Fibroblasts. Cell Reprogram. 2016;18:67-77 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 2	Mao C, Agca C, Mocko Strand J, Wang J, Ullrich LÃ¼ter E, Pan P, et al. Substituting mouse transcription factor Pou4f2 with a sea urchin orthologue restores retinal ganglion cell development. Proc Biol Sci. 2016;283:20152978 pubmed publisher
western blot; mouse; 1:500; fig 6	Bian Y, Yang L, Wang Z, Wang Q, Zeng L, Xu G. Repeated Three-Hour Maternal Separation Induces Depression-Like Behavior and Affects the Expression of Hippocampal Plasticity-Related Proteins in C57BL/6N Mice. Neural Plast. 2015;2015:627837 pubmed publisher
western blot; mouse; 1:500	Chen L, Dai J, Wang Z, Zhang H, Huang Y, Zhao Y. Ginseng Total Saponins Reverse Corticosterone-Induced Changes in Depression-Like Behavior and Hippocampal Plasticity-Related Proteins by Interfering with GSK-3 ? -CREB Signaling Pathway. Evid Based Complement Alternat Med. 2014;2014:506735 pubmed publisher
western blot; mouse; 1:500; fig 4	Zhao Y, Wang Z, Dai J, Chen L, Huang Y, Zhan Z. Beneficial effects of benzodiazepine diazepam on chronic stress-induced impairment of hippocampal structural plasticity and depression-like behavior in mice. Behav Brain Res. 2012;228:339-50 pubmed publisher
immunohistochemistry; human; tbl 2	Huizinga R, van der Star B, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; fig 1	Moshiri A, Gonzalez E, Tagawa K, Maeda H, Wang M, Frishman L, et al. Near complete loss of retinal ganglion cells in the math5/brn3b double knockout elicits severe reductions of other cell types during retinal development. Dev Biol. 2008;316:214-27 pubmed publisher
western blot; human	Kristiansen L, Beneyto M, Haroutunian V, Meador Woodruff J. Changes in NMDA receptor subunits and interacting PSD proteins in dorsolateral prefrontal and anterior cingulate cortex indicate abnormal regional expression in schizophrenia. Mol Psychiatry. 2006;11:737-47, 705 pubmed
immunohistochemistry - paraffin section; human; 1:1000; fig 3	Katayama S, Watanabe C, Noda K, Ohishi H, Yamamura Y, Nishisaka T, et al. Numerous conglomerate inclusions in slowly progressive familial amyotrophic lateral sclerosis with posterior column involvement. J Neurol Sci. 1999;171:72-7 pubmed
	Henderson M, Chung C, Riddle D, Zhang B, Gathagan R, Seeholzer S, et al. Unbiased Proteomics of Early Lewy Body Formation Model Implicates Active Microtubule Affinity-Regulating Kinases (MARKs) in Synucleinopathies. J Neurosci. 2017;37:5870-5884 pubmed publisher
	Nguyen M, Shu T, Sanada K, Lariviere R, Tseng H, Park S, et al. A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons. Nat Cell Biol. 2004;6:595-608 pubmed
	Haddad L, Smith N, Bowser M, Niida Y, Murthy V, Gonzalez Agosti C, et al. The TSC1 tumor suppressor hamartin interacts with neurofilament-L and possibly functions as a novel integrator of the neuronal cytoskeleton. J Biol Chem. 2002;277:44180-6 pubmed
	Wang S, Mu X, Bowers W, Kim D, Plas D, Crair M, et al. Brn3b/Brn3c double knockout mice reveal an unsuspected role for Brn3c in retinal ganglion cell axon outgrowth. Development. 2002;129:467-77 pubmed

product information

Product Type :

Antibody

Product Name :

NEFL Monoclonal Antibody (DA2)

Catalog # :

13-0400

Quantity :

200 µg

Price :

425 USD

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 0.1-0.5 µg/mL, Immunocytochemistry: 1:20, Immunofluorescence: 1:20, Immunohistochemistry (Paraffin): 1:20, Immunohistochemistry: 1:20 - 1:100, Immunoprecipitation: 2-5 µg, Western Blot: 0.5-2 µg/mL

Species :

Human, Mouse, Rat

Clone :

DA2

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

Immunogen :

Specifically recognizes a phosphate-independent epitope on NF-L.

Format :

Liquid

Applications w/Dilutions :

Aliases :

68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; Nf68; NFL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110

more info or order :

Invitrogen product webpage