product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GFAP Monoclonal Antibody (2.2B10)
catalog :
13-0300
quantity :
100 µg
price :
US 402.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
2.2B10
reactivity :
guinea pig, human, mouse, rat, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, immunohistochemistry knockout validation
more info or order :
citations: 202
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig 2c
Gaja Capdevila N, Hernández N, Zamanillo D, Vela J, Merlos M, Navarro X, et al. Neuroprotective Effects of Sigma 1 Receptor Ligands on Motoneuron Death after Spinal Root Injury in Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • immunohistochemistry; mouse; 1:300; loading ...; fig 5f
Villanueva E, Tresse E, Liu Y, Duarte J, Jimenez Duran G, Ejlerskov P, et al. Neuronal TNFα, Not α-Syn, Underlies PDD-Like Disease Progression in IFNβ-KO Mice. Ann Neurol. 2021;90:789-807 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 2c
Haan N, Westacott L, Carter J, Owen M, Gray W, Hall J, et al. Haploinsufficiency of the schizophrenia and autism risk gene Cyfip1 causes abnormal postnatal hippocampal neurogenesis through microglial and Arp2/3 mediated actin dependent mechanisms. Transl Psychiatry. 2021;11:313 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 1s1i
Li Y, Ritchie E, Steinke C, Qi C, Chen L, Zheng B, et al. Activation of MAP3K DLK and LZK in Purkinje cells causes rapid and slow degeneration depending on signaling strength. elife. 2021;10: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 6h
Morse S, Boltersdorf T, Harriss B, Chan T, Baxan N, Jung H, et al. Neuron labeling with rhodamine-conjugated Gd-based MRI contrast agents delivered to the brain via focused ultrasound. Theranostics. 2020;10:2659-2674 pubmed publisher
  • immunocytochemistry; rat; 1:500-1:1000; loading ...; fig 4i, 4j, s4b
Wall C, Rose C, Adrian M, Zeng Y, Kirkpatrick D, Bingol B. PPEF2 Opposes PINK1-Mediated Mitochondrial Quality Control by Dephosphorylating Ubiquitin. Cell Rep. 2019;29:3280-3292.e7 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:100; loading ...; fig s4b
Ising C, Venegas C, Zhang S, Scheiblich H, Schmidt S, Vieira Saecker A, et al. NLRP3 inflammasome activation drives tau pathology. Nature. 2019;: pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 1a
Blomfield I, Rocamonde B, Masdeu M, Mulugeta E, Vaga S, van den Berg D, et al. Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells. elife. 2019;8: pubmed publisher
  • immunohistochemistry - frozen section; human; 1:1000; loading ...; fig e5b
Schirmer L, Velmeshev D, Holmqvist S, Kaufmann M, Werneburg S, Jung D, et al. Neuronal vulnerability and multilineage diversity in multiple sclerosis. Nature. 2019;573:75-82 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2a
Joy M, Ben Assayag E, Shabashov Stone D, Liraz Zaltsman S, Mazzitelli J, Arenas M, et al. CCR5 Is a Therapeutic Target for Recovery after Stroke and Traumatic Brain Injury. Cell. 2019;176:1143-1157.e13 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 2d
Betlazar C, Harrison Brown M, Middleton R, Banati R, Liu G. Cellular Sources and Regional Variations in the Expression of the Neuroinflammatory Marker Translocator Protein (TSPO) in the Normal Brain. Int J Mol Sci. 2018;19: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1c
  • western blot; mouse; loading ...; fig 1d
Sato J, Horibe S, Kawauchi S, Sasaki N, Hirata K, Rikitake Y. Involvement of aquaporin-4 in laminin-enhanced process formation of mouse astrocytes in 2D culture: Roles of dystroglycan and α-syntrophin in aquaporin-4 expression. J Neurochem. 2018;147:495-513 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1d
Zhao C, Dong C, Frah M, Deng Y, Marie C, Zhang F, et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell. 2018;45:753-768.e8 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 1c
  • immunohistochemistry; human; 1:250; loading ...; fig 1a
Reichenbach N, Delekate A, Breithausen B, Keppler K, Poll S, Schulte T, et al. P2Y1 receptor blockade normalizes network dysfunction and cognition in an Alzheimer's disease model. J Exp Med. 2018;215:1649-1663 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 4a, 5c
Zukor K, Wang H, Siddharthan V, Julander J, Morrey J. Zika virus-induced acute myelitis and motor deficits in adult interferon ??/? receptor knockout mice. J Neurovirol. 2018;24:273-290 pubmed publisher
  • western blot; mouse; 1:500; fig s1b
Yungher B, Ribeiro M, Park K. Regenerative Responses and Axon Pathfinding of Retinal Ganglion Cells in Chronically Injured Mice. Invest Ophthalmol Vis Sci. 2017;58:1743-1750 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 12a
Zhu Y, Lyapichev K, Lee D, Motti D, Ferraro N, Zhang Y, et al. Macrophage Transcriptional Profile Identifies Lipid Catabolic Pathways That Can Be Therapeutically Targeted after Spinal Cord Injury. J Neurosci. 2017;37:2362-2376 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1f
Liddelow S, Guttenplan K, Clarke L, Bennett F, Bohlen C, Schirmer L, et al. Neurotoxic reactive astrocytes are induced by activated microglia. Nature. 2017;541:481-487 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig s1a
Behm M, Wahlstedt H, Widmark A, Eriksson M, Ohman M. Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development. J Cell Sci. 2017;130:745-753 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 3
Kim J, Lee J, Sun W. Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone. J Vis Exp. 2016;: pubmed publisher
  • immunohistochemistry; mouse; fig 8m
Ji B, Kaneko H, Minamimoto T, Inoue H, Takeuchi H, Kumata K, et al. Multimodal Imaging for DREADD-Expressing Neurons in Living Brain and Their Application to Implantation of iPSC-Derived Neural Progenitors. J Neurosci. 2016;36:11544-11558 pubmed
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 6d
Song D, Wilson B, Zhao L, Bhuyan R, Bandyopadhyay M, Lyubarsky A, et al. Retinal Pre-Conditioning by CD59a Knockout Protects against Light-Induced Photoreceptor Degeneration. PLoS ONE. 2016;11:e0166348 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; tbl 1
Mildner A, Huang H, Radke J, Stenzel W, Priller J. P2Y12 receptor is expressed on human microglia under physiological conditions throughout development and is sensitive to neuroinflammatory diseases. Glia. 2017;65:375-387 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 4a
Menzel L, Kleber L, Friedrich C, Hummel R, Dangel L, Winter J, et al. Progranulin protects against exaggerated axonal injury and astrogliosis following traumatic brain injury. Glia. 2017;65:278-292 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; tbl 1
Zukor K, Wang H, Hurst B, Siddharthan V, van Wettere A, Pilowsky P, et al. Phrenic nerve deficits and neurological immunopathology associated with acute West Nile virus infection in mice and hamsters. J Neurovirol. 2017;23:186-204 pubmed publisher
  • immunohistochemistry; mouse; 1:5000; loading ...; fig 5a
Koyanagi S, Kusunose N, Taniguchi M, Akamine T, Kanado Y, Ozono Y, et al. Glucocorticoid regulation of ATP release from spinal astrocytes underlies diurnal exacerbation of neuropathic mechanical allodynia. Nat Commun. 2016;7:13102 pubmed publisher
  • immunocytochemistry; human; fig 1g
Zhang L, Hua Q, Tang K, Shi C, Xie X, Zhang R. CXCR4 activation promotes differentiation of human embryonic stem cells to neural stem cells. Neuroscience. 2016;337:88-97 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 5a
Barron A, Tokunaga M, Zhang M, Ji B, Suhara T, Higuchi M. Assessment of neuroinflammation in a mouse model of obesity and β-amyloidosis using PET. J Neuroinflammation. 2016;13:221 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400; fig 2
Hillis J, Davies J, Mundim M, Al Dalahmah O, Szele F. Cuprizone demyelination induces a unique inflammatory response in the subventricular zone. J Neuroinflammation. 2016;13:190 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 7b
Badea A, Kane L, Anderson R, Qi Y, Foster M, Cofer G, et al. The fornix provides multiple biomarkers to characterize circuit disruption in a mouse model of Alzheimer's disease. Neuroimage. 2016;142:498-511 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; fig 3
Saggu R, Schumacher T, Gerich F, Rakers C, Tai K, Delekate A, et al. Astroglial NF-kB contributes to white matter damage and cognitive impairment in a mouse model of vascular dementia. Acta Neuropathol Commun. 2016;4:76 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 1f
Urbán N, van den Berg D, Forget A, Andersen J, Demmers J, Hunt C, et al. Return to quiescence of mouse neural stem cells by degradation of a proactivation protein. Science. 2016;353:292-5 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3a
Walker W, Oehler A, Edinger A, Wagner K, Gunn T. Oligodendroglial deletion of ESCRT-I component TSG101 causes spongiform encephalopathy. Biol Cell. 2016;108:324-337 pubmed publisher
  • immunohistochemistry; mouse; 1:2000; tbl 1
Park K, Luo X, Mooney S, Yungher B, Belin S, Wang C, et al. Retinal ganglion cell survival and axon regeneration after optic nerve injury in naked mole-rats. J Comp Neurol. 2017;525:380-388 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig s2
Vasek M, Garber C, Dorsey D, Durrant D, Bollman B, Soung A, et al. A complement-microglial axis drives synapse loss during virus-induced memory impairment. Nature. 2016;534:538-43 pubmed publisher
  • immunocytochemistry; mouse; fig 1
Kizuka Y, Nakano M, Miura Y, Taniguchi N. Epigenetic regulation of neural N-glycomics. Proteomics. 2016;16:2854-2863 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 2
Funk L, Hackett A, Bunge M, Lee J. Tumor necrosis factor superfamily member APRIL contributes to fibrotic scar formation after spinal cord injury. J Neuroinflammation. 2016;13:87 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 3
Smeester B, O Brien E, Michlitsch K, Lee J, Beitz A. The relationship of bone-tumor-induced spinal cord astrocyte activation and aromatase expression to mechanical hyperalgesia and cold hypersensitivity in intact female and ovariectomized mice. Neuroscience. 2016;324:344-54 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 1c
Hackett A, Lee D, Dawood A, Rodriguez M, Funk L, Tsoulfas P, et al. STAT3 and SOCS3 regulate NG2 cell proliferation and differentiation after contusive spinal cord injury. Neurobiol Dis. 2016;89:10-22 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:6000; fig 1
Hristova M, Rocha Ferreira E, Fontana X, Thei L, Buckle R, Christou M, et al. Inhibition of Signal Transducer and Activator of Transcription 3 (STAT3) reduces neonatal hypoxic-ischaemic brain damage. J Neurochem. 2016;136:981-94 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
Wang S, Hsu J, Ko C, Chiu N, Kan W, Lai M, et al. Astrocytic CCAAT/Enhancer-Binding Protein Delta Contributes to Glial Scar Formation and Impairs Functional Recovery After Spinal Cord Injury. Mol Neurobiol. 2016;53:5912-5927 pubmed publisher
  • immunocytochemistry; mouse
Kizuka Y, Nakano M, Kitazume S, Saito T, Saido T, Taniguchi N. Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem J. 2016;473:21-30 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200
Fredriksson L, Stevenson T, Su E, Ragsdale M, Moore S, Craciun S, et al. Identification of a neurovascular signaling pathway regulating seizures in mice. Ann Clin Transl Neurol. 2015;2:722-38 pubmed publisher
  • immunohistochemistry; mouse; 1:2000
Chen Y, Huang W, Séjourné J, Clipperton Allen A, Page D. Pten Mutations Alter Brain Growth Trajectory and Allocation of Cell Types through Elevated β-Catenin Signaling. J Neurosci. 2015;35:10252-67 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:300
Puntambekar S, Hinton D, Yin X, Savarin C, Bergmann C, Trapp B, et al. Interleukin-10 is a critical regulator of white matter lesion containment following viral induced demyelination. Glia. 2015;63:2106-2120 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 1a
Schachtrup C, Ryu J, Mammadzada K, Khan A, Carlton P, Perez A, et al. Nuclear pore complex remodeling by p75(NTR) cleavage controls TGF-β signaling and astrocyte functions. Nat Neurosci. 2015;18:1077-80 pubmed publisher
  • immunohistochemistry; mouse; 1:1000
O Brien E, Smeester B, Michlitsch K, Lee J, Beitz A. Colocalization of aromatase in spinal cord astrocytes: differences in expression and relationship to mechanical and thermal hyperalgesia in murine models of a painful and a non-painful bone tumor. Neuroscience. 2015;301:235-45 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
Shin C, Grossmann A, Holmen S, Robinson J. The BRAF kinase domain promotes the development of gliomas in vivo. Genes Cancer. 2015;6:9-18 pubmed
  • immunocytochemistry; human; 1:1000
Crouch E, Liu C, Silva Vargas V, Doetsch F. Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage. J Neurosci. 2015;35:4528-39 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 2
Chen Roetling J, Song W, Schipper H, Regan C, Regan R. Astrocyte overexpression of heme oxygenase-1 improves outcome after intracerebral hemorrhage. Stroke. 2015;46:1093-8 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Cantoni C, Bollman B, Licastro D, Xie M, Mikesell R, Schmidt R, et al. TREM2 regulates microglial cell activation in response to demyelination in vivo. Acta Neuropathol. 2015;129:429-47 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig s1c
Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, et al. An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease. EMBO Mol Med. 2015;7:175-89 pubmed publisher
  • immunohistochemistry; rat; 1:2000
  • immunohistochemistry; mouse; 1:2000
Zhu Y, Soderblom C, Trojanowsky M, Lee D, Lee J. Fibronectin Matrix Assembly after Spinal Cord Injury. J Neurotrauma. 2015;32:1158-67 pubmed publisher
  • immunohistochemistry; mouse; 1:2000
Zhu Y, Soderblom C, Krishnan V, Ashbaugh J, Bethea J, Lee J. Hematogenous macrophage depletion reduces the fibrotic scar and increases axonal growth after spinal cord injury. Neurobiol Dis. 2015;74:114-25 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 5
Wu C, Hung T, Chen C, Ke C, Lee C, Wang P, et al. Post-injury treatment with 7,8-dihydroxyflavone, a TrkB receptor agonist, protects against experimental traumatic brain injury via PI3K/Akt signaling. PLoS ONE. 2014;9:e113397 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig s1
Kawase S, Kuwako K, Imai T, Renault Mihara F, Yaguchi K, Itohara S, et al. Regulatory factor X transcription factors control Musashi1 transcription in mouse neural stem/progenitor cells. Stem Cells Dev. 2014;23:2250-61 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250
Howerton A, Roland A, Bale T. Dorsal raphe neuroinflammation promotes dramatic behavioral stress dysregulation. J Neurosci. 2014;34:7113-23 pubmed publisher
  • immunohistochemistry - frozen section; mouse
  • western blot; mouse
Wakatsuki S, Araki T, Sehara Fujisawa A. Neuregulin-1/glial growth factor stimulates Schwann cell migration by inducing ?5 ?1 integrin-ErbB2-focal adhesion kinase complex formation. Genes Cells. 2014;19:66-77 pubmed publisher
  • western blot; mouse; 1:1000
Cops E, Sashindranath M, Daglas M, Short K, da Fonseca Pereira C, Pang T, et al. Tissue-type plasminogen activator is an extracellular mediator of Purkinje cell damage and altered gait. Exp Neurol. 2013;249:8-19 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 1
Karasinska J, de Haan W, Franciosi S, Ruddle P, Fan J, Kruit J, et al. ABCA1 influences neuroinflammation and neuronal death. Neurobiol Dis. 2013;54:445-55 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 5
Phares T, Stohlman S, Hinton D, Bergmann C. Astrocyte-derived CXCL10 drives accumulation of antibody-secreting cells in the central nervous system during viral encephalomyelitis. J Virol. 2013;87:3382-92 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 1
Chen S, Tsai H, Hung T, Chen C, Lee C, Wu C, et al. Salidroside improves behavioral and histological outcomes and reduces apoptosis via PI3K/Akt signaling after experimental traumatic brain injury. PLoS ONE. 2012;7:e45763 pubmed publisher
  • immunohistochemistry; mouse; 1:250; fig 2
Gerber A, Bale T. Antiinflammatory treatment ameliorates HPA stress axis dysfunction in a mouse model of stress sensitivity. Endocrinology. 2012;153:4830-7 pubmed
  • immunohistochemistry - frozen section; mouse; 1:250; fig s1
Dixon K, Munro K, Boyd A, Bartlett P, Turnley A. Partial change in EphA4 knockout mouse phenotype: loss of diminished GFAP upregulation following spinal cord injury. Neurosci Lett. 2012;525:66-71 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:100; fig 2
  • immunohistochemistry - paraffin section; human; 1:100; fig 2
Skjolding A, Holst A, Broholm H, Laursen H, Juhler M. Differences in distribution and regulation of astrocytic aquaporin-4 in human and rat hydrocephalic brain. Neuropathol Appl Neurobiol. 2013;39:179-91 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 2
Lutz S, Raine C, Brosnan C. Loss of astrocyte connexins 43 and 30 does not significantly alter susceptibility or severity of acute experimental autoimmune encephalomyelitis in mice. J Neuroimmunol. 2012;245:8-14 pubmed publisher
  • immunohistochemistry; rat; fig 5
Lewitus D, Landers J, Branch J, Smith K, Callegari G, Kohn J, et al. Biohybrid Carbon Nanotube/Agarose Fibers for Neural Tissue Engineering. Adv Funct Mater. 2011;21:2624-2632 pubmed
  • immunohistochemistry - frozen section; mouse; fig 5
Zhao L, Ma W, Fariss R, Wong W. Minocycline attenuates photoreceptor degeneration in a mouse model of subretinal hemorrhage microglial: inhibition as a potential therapeutic strategy. Am J Pathol. 2011;179:1265-77 pubmed publisher
  • immunohistochemistry; human; 1:200; fig 4
Lewitus D, Smith K, Shain W, Bolikal D, Kohn J. The fate of ultrafast degrading polymeric implants in the brain. Biomaterials. 2011;32:5543-50 pubmed publisher
  • immunohistochemistry; human; 1:400
Chang C, Chen S, Lee T, Lee H, Chen S, Shyue S. Caveolin-1 deletion reduces early brain injury after experimental intracerebral hemorrhage. Am J Pathol. 2011;178:1749-61 pubmed publisher
  • immunohistochemistry; rat; 1:200; fig 7
Lewitus D, Smith K, Shain W, Kohn J. Ultrafast resorbing polymers for use as carriers for cortical neural probes. Acta Biomater. 2011;7:2483-91 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 5
Phares T, Marques C, Stohlman S, Hinton D, Bergmann C. Factors supporting intrathecal humoral responses following viral encephalomyelitis. J Virol. 2011;85:2589-98 pubmed publisher
  • western blot; mouse; fig s1
Yang H, Zhuo J, Chu J, Chinnici C, Pratico D. Amelioration of the Alzheimer's disease phenotype by absence of 12/15-lipoxygenase. Biol Psychiatry. 2010;68:922-9 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 1
DellaValle B, Hempel C, Kurtzhals J, Penkowa M. In vivo expression of neuroglobin in reactive astrocytes during neuropathology in murine models of traumatic brain injury, cerebral malaria, and autoimmune encephalitis. Glia. 2010;58:1220-7 pubmed publisher
  • immunohistochemistry; rat; 1:1000; fig 2
Pang J, Gao F, Wu S. Light responses and morphology of bNOS-immunoreactive neurons in the mouse retina. J Comp Neurol. 2010;518:2456-74 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig s4
VanBrocklin M, Robinson J, Lastwika K, Khoury J, Holmen S. Targeted delivery of NRASQ61R and Cre-recombinase to post-natal melanocytes induces melanoma in Ink4a/Arflox/lox mice. Pigment Cell Melanoma Res. 2010;23:531-41 pubmed publisher
  • immunohistochemistry - frozen section; mouse
  • immunocytochemistry; mouse
Ji B, Maeda J, Sawada M, Ono M, Okauchi T, Inaji M, et al. Imaging of peripheral benzodiazepine receptor expression as biomarkers of detrimental versus beneficial glial responses in mouse models of Alzheimer's and other CNS pathologies. J Neurosci. 2008;28:12255-67 pubmed publisher
  • immunohistochemistry; guinea pig; 1:100-1:200
  • immunohistochemistry; human; 1:100-1:200
Hoff S, Zeller F, Von Weyhern C, Wegner M, Schemann M, Michel K, et al. Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8/9/10 antibody. J Comp Neurol. 2008;509:356-71 pubmed publisher
  • immunohistochemistry; mouse; fig 8
Blakqori G, Delhaye S, Habjan M, Blair C, S nchez Vargas I, Olson K, et al. La Crosse bunyavirus nonstructural protein NSs serves to suppress the type I interferon system of mammalian hosts. J Virol. 2007;81:4991-9 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:3000; tbl 2
Herber D, Maloney J, Roth L, Freeman M, Morgan D, Gordon M. Diverse microglial responses after intrahippocampal administration of lipopolysaccharide. Glia. 2006;53:382-91 pubmed
  • immunoprecipitation; mouse
Wicher G, Larsson M, Rask L, Aldskogius H. Low-density lipoprotein receptor-related protein (LRP)-2/megalin is transiently expressed in a subpopulation of neural progenitors in the embryonic mouse spinal cord. J Comp Neurol. 2005;492:123-31 pubmed
  • immunohistochemistry - free floating section; mouse; 1:3000; tbl 1
Herber D, Roth L, Wilson D, Wilson N, Mason J, Morgan D, et al. Time-dependent reduction in Abeta levels after intracranial LPS administration in APP transgenic mice. Exp Neurol. 2004;190:245-53 pubmed
  • immunohistochemistry; mouse; 1:10,000; fig 1
  • western blot; mouse; 1:1000; fig 1
Apicelli A, Uhlmann E, Baldwin R, Ding H, Nagy A, Guha A, et al. Role of the Rap1 GTPase in astrocyte growth regulation. Glia. 2003;42:225-34 pubmed
  • immunohistochemistry - free floating section; mouse; 1:10000
  • western blot; mouse; 1:1000
Uhlmann E, Apicelli A, Baldwin R, Burke S, Bajenaru M, Onda H, et al. Heterozygosity for the tuberous sclerosis complex (TSC) gene products results in increased astrocyte numbers and decreased p27-Kip1 expression in TSC2+/- cells. Oncogene. 2002;21:4050-9 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:2; fig 3
Seitz A, Aglow E, Heber Katz E. Recovery from spinal cord injury: a new transection model in the C57Bl/6 mouse. J Neurosci Res. 2002;67:337-45 pubmed
  • immunohistochemistry; mouse; 1:100
Penkowa M, Carrasco J, Giralt M, Moos T, Hidalgo J. CNS wound healing is severely depressed in metallothionein I- and II-deficient mice. J Neurosci. 1999;19:2535-45 pubmed
  • immunocytochemistry; mouse; fig 3
Satoh J, Yukitake M, Kuroda Y. Constitutive and heat-inducible expression of HSP105 in neurons and glial cells in culture. Neuroreport. 1998;9:2977-83 pubmed
Shil S, Kagawa Y, Umaru B, Nanto Hara F, Miyazaki H, Yamamoto Y, et al. Ndufs4 ablation decreases synaptophysin expression in hippocampus. Sci Rep. 2021;11:10969 pubmed publisher
Shariq M, Sahasrabuddhe V, Krishna S, Radha S, Nruthyathi -, Bellampalli R, et al. Adult neural stem cells have latent inflammatory potential that is kept suppressed by Tcf4 to facilitate adult neurogenesis. Sci Adv. 2021;7: pubmed publisher
Mallah K, Couch C, Alshareef M, Borucki D, Yang X, Alawieh A, et al. Complement mediates neuroinflammation and cognitive decline at extended chronic time points after traumatic brain injury. Acta Neuropathol Commun. 2021;9:72 pubmed publisher
Woodburn S, Bollinger J, Wohleb E. Synaptic and behavioral effects of chronic stress are linked to dynamic and sex-specific changes in microglia function and astrocyte dystrophy. Neurobiol Stress. 2021;14:100312 pubmed publisher
Xiao D, Zhang W, Wang Q, Li X, Zhang Y, Rasouli J, et al. CRISPR-mediated rapid generation of neural cell-specific knockout mice facilitates research in neurophysiology and pathology. Mol Ther Methods Clin Dev. 2021;20:755-764 pubmed publisher
Manganas L, Durá I, Osenberg S, Semerci F, Tosun M, Mishra R, et al. BASP1 labels neural stem cells in the neurogenic niches of mammalian brain. Sci Rep. 2021;11:5546 pubmed publisher
Shin H, Jeong S, Lee J, Sun W, Choi N, Cho I. 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics. Nat Commun. 2021;12:492 pubmed publisher
Nitschke S, Chown E, Zhao X, Gabrielian S, Petković S, Guisso D, et al. An inducible glycogen synthase-1 knockout halts but does not reverse Lafora disease progression in mice. J Biol Chem. 2021;296:100150 pubmed publisher
Loy K, Fourneau J, Meng N, Denecke C, Locatelli G, Bareyre F. Semaphorin 7A restricts serotonergic innervation and ensures recovery after spinal cord injury. Cell Mol Life Sci. 2021;78:2911-2927 pubmed publisher
Bae Y, Choi J, Ryoo K, Kim A, Kwon O, Jung H, et al. Spadin Modulates Astrocytic Passive Conductance via Inhibition of TWIK-1/TREK-1 Heterodimeric Channels. Int J Mol Sci. 2020;21: pubmed publisher
OʼShea T, Wollenberg A, Kim J, Ao Y, Deming T, Sofroniew M. Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions. Nat Commun. 2020;11:6203 pubmed publisher
Sigfridsson E, Marangoni M, Hardingham G, Horsburgh K, Fowler J. Deficiency of Nrf2 exacerbates white matter damage and microglia/macrophage levels in a mouse model of vascular cognitive impairment. J Neuroinflammation. 2020;17:367 pubmed publisher
Still K, Batista S, O Brien C, Oyesola O, Früh S, Webb L, et al. Astrocytes promote a protective immune response to brain Toxoplasma gondii infection via IL-33-ST2 signaling. PLoS Pathog. 2020;16:e1009027 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
GFAP Monoclonal Antibody (2.2B10)
Catalog # :
13-0300
Quantity :
100 µg
Price :
US 402.00
Clonality :
Monoclonal
Purity :
purified
Host :
Rat
Reactivity :
Bovine, Human, Mouse, Rat
Applications :
ELISA: 0.1-0.5 µg/mL, Flow Cytometry: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry: 10-50 µg/mL, Immunoprecipitation: 2-5 µg, Western Blot: Assay-dependent
Species :
Bovine, Human, Mouse, Rat
Clone :
2.2B10
Isotype :
IgG2a, kappa
Storage :
-20°C
Description :
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
Immunogen :
Enriched bovine glial filaments.
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-0.5 µg/mL, Flow Cytometry: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry: 10-50 µg/mL, Immunoprecipitation: 2-5 µg, Western Blot: Assay-dependent
Aliases :
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA