Amyloid Precursor Protein Monoclonal Antibody (LN27) | Invitrogen 13-0200 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Amyloid Precursor Protein Monoclonal Antibody (LN27)

catalog :

13-0200

quantity :

100 µg

price :

US 482

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LN27

reactivity :

pigtail macaque, human, mouse, rat, domestic sheep, domestic rabbit, rhesus macaque

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 55

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; fig 3	Hares K, Redondo J, Kemp K, Rice C, Scolding N, Wilkins A. Axonal motor protein KIF5A and associated cargo deficits in multiple sclerosis lesional and normal-appearing white matter. Neuropathol Appl Neurobiol. 2017;43:227-241 pubmed publisher
immunohistochemistry; human; fig 7	Yuan P, Grutzendler J. Attenuation of Î²-Amyloid Deposition and Neurotoxicity by Chemogenetic Modulation of Neural Activity. J Neurosci. 2016;36:632-41 pubmed publisher
western blot; human; 1:400	Chun Y, Oh H, Park M, Kim T, Chung S. Increasing Membrane Cholesterol Level Increases the Amyloidogenic Peptide by Enhancing the Expression of Phospholipase C. J Neurodegener Dis. 2013;2013:407903 pubmed publisher
western blot; mouse; 1:1000; fig 4	Lee I, Jung K, Kim I, Lee H, Kim M, Yun S, et al. Human neural stem cells alleviate Alzheimer-like pathology in a mouse model. Mol Neurodegener. 2015;10:38 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100	Reis R, Hennessy E, Murray C, Griffin Ã, Cunningham C. At the centre of neuronal, synaptic and axonal pathology in murine prion disease: degeneration of neuroanatomically linked thalamic and brainstem nuclei. Neuropathol Appl Neurobiol. 2015;41:780-97 pubmed publisher
immunohistochemistry; rhesus macaque; fig 4	Kelly K, Beck S, Metcalf Pate K, Queen S, Dorsey J, Adams R, et al. Neuroprotective maraviroc monotherapy in simian immunodeficiency virus-infected macaques: reduced replicating and latent SIV in the brain. AIDS. 2013;27:F21-8 pubmed publisher
western blot; human; fig 1	Kang M, Baek S, Chun Y, Moore A, Landman N, Berman D, et al. Modulation of lipid kinase PI4KII? activity and lipid raft association of presenilin 1 underlies ?-secretase inhibition by ginsenoside (20S)-Rg3. J Biol Chem. 2013;288:20868-82 pubmed publisher
immunocytochemistry; human	Zheng L, Cedazo Minguez A, Hallbeck M, Jerhammar F, Marcusson J, Terman A. Intracellular distribution of amyloid beta peptide and its relationship to the lysosomal system. Transl Neurodegener. 2012;1:19 pubmed publisher
western blot; human; fig 6b	Saito Y, Inoue T, Zhu G, Kimura N, Okada M, Nishimura M, et al. Hyperpolarization-activated cyclic nucleotide gated channels: a potential molecular link between epileptic seizures and A? generation in Alzheimer's disease. Mol Neurodegener. 2012;7:50 pubmed publisher
western blot; human; 1:1000; fig 3, 4	Zheng Y, Wang Q, Xiao B, Lu Q, Wang Y, Wang X. Involvement of receptor tyrosine kinase Tyro3 in amyloidogenic APP processing and ?-amyloid deposition in Alzheimer's disease models. PLoS ONE. 2012;7:e39035 pubmed publisher
western blot; human; 1:250; fig 3	Killian R, Flippin J, Herrera C, Almenar Queralt A, Goldstein L. Kinesin light chain 1 suppression impairs human embryonic stem cell neural differentiation and amyloid precursor protein metabolism. PLoS ONE. 2012;7:e29755 pubmed publisher
immunohistochemistry; human; tbl 2	Huizinga R, van der Star B, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31 pubmed publisher
immunocytochemistry; human; 1:100; fig 1	Zheng L, Terman A, Hallbeck M, Dehvari N, Cowburn R, Benedikz E, et al. Macroautophagy-generated increase of lysosomal amyloid β-protein mediates oxidant-induced apoptosis of cultured neuroblastoma cells. Autophagy. 2011;7:1528-45 pubmed
western blot; human; fig 1	Chapuis J, Vingtdeux V, Capiralla H, Davies P, Marambaud P. Gas1 interferes with A?PP trafficking by facilitating the accumulation of immature A?PP in endoplasmic reticulum-associated raft subdomains. J Alzheimers Dis. 2012;28:127-35 pubmed publisher
immunocytochemistry; mouse; fig 7	Ranganathan S, Noyes N, Migliorini M, Winkles J, Battey F, Hyman B, et al. LRAD3, a novel low-density lipoprotein receptor family member that modulates amyloid precursor protein trafficking. J Neurosci. 2011;31:10836-46 pubmed publisher
immunocytochemistry; human western blot; human	Chapuis J, Vingtdeux V, Campagne F, Davies P, Marambaud P. Growth arrest-specific 1 binds to and controls the maturation and processing of the amyloid-beta precursor protein. Hum Mol Genet. 2011;20:2026-36 pubmed publisher
western blot; human	van Noort J, Bsibsi M, Gerritsen W, van der Valk P, Bajramovic J, Steinman L, et al. Alphab-crystallin is a target for adaptive immune responses and a trigger of innate responses in preactive multiple sclerosis lesions. J Neuropathol Exp Neurol. 2010;69:694-703 pubmed publisher
immunocytochemistry; mouse	Vingtdeux V, Giliberto L, Zhao H, Chandakkar P, Wu Q, Simon J, et al. AMP-activated protein kinase signaling activation by resveratrol modulates amyloid-beta peptide metabolism. J Biol Chem. 2010;285:9100-13 pubmed publisher
western blot; human	Portelius E, Brinkmalm G, Tran A, Andreasson U, Zetterberg H, Westman Brinkmalm A, et al. Identification of novel N-terminal fragments of amyloid precursor protein in cerebrospinal fluid. Exp Neurol. 2010;223:351-8 pubmed publisher
immunohistochemistry - paraffin section; rhesus macaque; fig 1	Mankowski J, Queen S, Fernandez C, Tarwater P, Karper J, Adams R, et al. Natural host genetic resistance to lentiviral CNS disease: a neuroprotective MHC class I allele in SIV-infected macaques. PLoS ONE. 2008;3:e3603 pubmed publisher
immunohistochemistry; human; fig 1	Moeller J, Macaulay R, Valdmanis P, Weston L, Rouleau G, Dupre N. Autosomal dominant sensory ataxia: a neuroaxonal dystrophy. Acta Neuropathol. 2008;116:331-6 pubmed publisher
immunocytochemistry; human; fig 2	Ryoo S, Cho H, Lee H, Jeong H, Radnaabazar C, Kim Y, et al. Dual-specificity tyrosine(Y)-phosphorylation regulated kinase 1A-mediated phosphorylation of amyloid precursor protein: evidence for a functional link between Down syndrome and Alzheimer's disease. J Neurochem. 2008;104:1333-44 pubmed
immunohistochemistry - paraffin section; rat; 1:200	Ai J, Liu E, Wang J, Chen Y, Yu J, Baker A. Calpain inhibitor MDL-28170 reduces the functional and structural deterioration of corpus callosum following fluid percussion injury. J Neurotrauma. 2007;24:960-78 pubmed
ELISA; human; 0.5 ug/ml	Matsui T, Ingelsson M, Fukumoto H, Ramasamy K, Kowa H, Frosch M, et al. Expression of APP pathway mRNAs and proteins in Alzheimer's disease. Brain Res. 2007;1161:116-23 pubmed
immunocytochemistry; human; 1:1000	Nizzari M, Venezia V, Repetto E, Caorsi V, Magrassi R, Gagliani M, et al. Amyloid precursor protein and Presenilin1 interact with the adaptor GRB2 and modulate ERK 1,2 signaling. J Biol Chem. 2007;282:13833-44 pubmed
immunohistochemistry - paraffin section; human; 1:1500 immunohistochemistry - paraffin section; mouse; 1:1500; fig 8	German A, Myint K, Mai N, Pomeroy I, Phu N, Tzartos J, et al. A preliminary neuropathological study of Japanese encephalitis in humans and a mouse model. Trans R Soc Trop Med Hyg. 2006;100:1135-45 pubmed
western blot; rat; fig 2	Kirazov L, Kirazovi E, Schliebs R. L-glutamate and phorbol ester stimulate the release of secretory amyloid precursor protein from rat cortical synaptosomes. Acta Biol Hung. 2005;56:177-83 pubmed
immunohistochemistry; rhesus macaque; 1:10,000	Helke K, Queen S, Tarwater P, Turchan Cholewo J, Nath A, Zink M, et al. 14-3-3 protein in CSF: an early predictor of SIV CNS disease. J Neuropathol Exp Neurol. 2005;64:202-8 pubmed
immunohistochemistry - paraffin section; human; fig 5	Sasaki K, Doh ura K, Wakisaka Y, Tomoda H, Iwaki T. Fatal familial insomnia with an unusual prion protein deposition pattern: an autopsy report with an experimental transmission study. Neuropathol Appl Neurobiol. 2005;31:80-7 pubmed
immunohistochemistry - paraffin section; mouse; fig 2 western blot; mouse; fig 2	Lee K, Lee S, Kim H, Song J, Yang S, Paik S, et al. Progressive cognitive impairment and anxiety induction in the absence of plaque deposition in C57BL/6 inbred mice expressing transgenic amyloid precursor protein. J Neurosci Res. 2004;76:572-80 pubmed
ELISA; human	Trysberg E, Höglund K, Svenungsson E, Blennow K, Tarkowski A. Decreased levels of soluble amyloid beta-protein precursor and beta-amyloid protein in cerebrospinal fluid of patients with systemic lupus erythematosus. Arthritis Res Ther. 2004;6:R129-36 pubmed
western blot; human; fig 1	Richardson J, Kendal C, Anderson R, Priest F, Gower E, Soden P, et al. Ultrastructural and behavioural changes precede amyloid deposition in a transgenic model of Alzheimer's disease. Neuroscience. 2003;122:213-28 pubmed
immunohistochemistry - paraffin section; domestic sheep; fig 3	Anderson R, Brown C, Blumbergs P, McLean A, Jones N. Impact mechanics and axonal injury in a sheep model. J Neurotrauma. 2003;20:961-74 pubmed
western blot; human; fig 3	Araki Y, Tomita S, Yamaguchi H, Miyagi N, Sumioka A, Kirino Y, et al. Novel cadherin-related membrane proteins, Alcadeins, enhance the X11-like protein-mediated stabilization of amyloid beta-protein precursor metabolism. J Biol Chem. 2003;278:49448-58 pubmed
immunohistochemistry; human; tbl 2	Weed M, Hienz R, Brady J, Adams R, Mankowski J, Clements J, et al. Central nervous system correlates of behavioral deficits following simian immunodeficiency virus infection. J Neurovirol. 2003;9:452-64 pubmed
immunohistochemistry; pigtail macaque; fig 1	Mankowski J, Queen S, Tarwater P, Fox K, Perry V. Accumulation of beta-amyloid precursor protein in axons correlates with CNS expression of SIV gp41. J Neuropathol Exp Neurol. 2002;61:85-90 pubmed
western blot; human; fig 6	Daugherty B, Green S. Endosomal sorting of amyloid precursor protein-P-selectin chimeras influences secretase processing. Traffic. 2001;2:908-16 pubmed
ELISA; human	Steinhilb M, Turner R, Gaut J. The protease inhibitor, MG132, blocks maturation of the amyloid precursor protein Swedish mutant preventing cleavage by beta-Secretase. J Biol Chem. 2001;276:4476-84 pubmed
immunoprecipitation; human; fig 3 western blot; human; fig 8	Lefterov I, Koldamova R, Lazo J. Human bleomycin hydrolase regulates the secretion of amyloid precursor protein. FASEB J. 2000;14:1837-47 pubmed
immunocytochemistry; human; fig 8	Tomita S, Ozaki T, Taru H, Oguchi S, Takeda S, Yagi Y, et al. Interaction of a neuron-specific protein containing PDZ domains with Alzheimer's amyloid precursor protein. J Biol Chem. 1999;274:2243-54 pubmed
immunohistochemistry - paraffin section; domestic rabbit; 1:200; fig 1 western blot; domestic rabbit; 1:1000; fig 2	Huang Y, Herman M, Liu J, Katsetos C, Wills M, Savory J. Neurofibrillary lesions in experimental aluminum-induced encephalopathy and Alzheimer's disease share immunoreactivity for amyloid precursor protein, A beta, alpha 1-antichymotrypsin and ubiquitin-protein conjugates. Brain Res. 1997;771:213-20 pubmed
immunohistochemistry - paraffin section; human; 1:200	Sherriff F, Bridges L, Gentleman S, Sivaloganathan S, Wilson S. Markers of axonal injury in post mortem human brain. Acta Neuropathol. 1994;88:433-9 pubmed
immunohistochemistry - paraffin section; human; 1:200	Webster M, Groome N, Francis P, Pearce B, Sherriff F, Thinakaran G, et al. A novel protein, amyloid precursor-like protein 2, is present in human brain, cerebrospinal fluid and conditioned media. Biochem J. 1995;310 ( Pt 1):95-9 pubmed
	Cejas R, Tamaño Blanco M, Blanco J. Analysis of the intracellular traffic of IgG in the context of Down syndrome (trisomy 21). Sci Rep. 2021;11:10981 pubmed publisher
	Chai Q, Jovasevic V, Malikov V, Sabo Y, MORHAM S, Walsh D, et al. HIV-1 counteracts an innate restriction by amyloid precursor protein resulting in neurodegeneration. Nat Commun. 2017;8:1522 pubmed publisher
	Mbah N, Overmeyer J, Maltese W. Disruption of endolysosomal trafficking pathways in glioma cells by methuosis-inducing indole-based chalcones. Cell Biol Toxicol. 2017;33:263-282 pubmed publisher
	Yuan P, Condello C, Keene C, Wang Y, Bird T, Paul S, et al. TREM2 Haplodeficiency in Mice and Humans Impairs the Microglia Barrier Function Leading to Decreased Amyloid Compaction and Severe Axonal Dystrophy. Neuron. 2016;90:724-39 pubmed publisher
	Anjum A, Brathwaite K, Aidley J, Connerton P, Cummings N, Parkhill J, et al. Phase variation of a Type IIG restriction-modification enzyme alters site-specific methylation patterns and gene expression in Campylobacter jejuni strain NCTC11168. Nucleic Acids Res. 2016;44:4581-94 pubmed publisher
	Wang J, Lu R, Yang J, Li H, He Z, Jing N, et al. TRPC6 specifically interacts with APP to inhibit its cleavage by Î³-secretase and reduce AÎ² production. Nat Commun. 2015;6:8876 pubmed publisher
	Cheng X, Gao Y, Zhao Y, Cheng X. Sodium Chloride Increases AÎ² Levels by Suppressing AÎ² Clearance in Cultured Cells. PLoS ONE. 2015;10:e0130432 pubmed publisher
	Willuweit A, Velden J, Godemann R, Manook A, Jetzek F, Tintrup H, et al. Early-onset and robust amyloid pathology in a new homozygous mouse model of Alzheimer's disease. PLoS ONE. 2009;4:e7931 pubmed publisher
	Bacher M, Dodel R, Aljabari B, Keyvani K, Marambaud P, Kayed R, et al. CNI-1493 inhibits Abeta production, plaque formation, and cognitive deterioration in an animal model of Alzheimer's disease. J Exp Med. 2008;205:1593-9 pubmed publisher
	Zambrano N, Gianni D, Bruni P, Passaro F, Telese F, Russo T. Fe65 is not involved in the platelet-derived growth factor-induced processing of Alzheimer's amyloid precursor protein, which activates its caspase-directed cleavage. J Biol Chem. 2004;279:16161-9 pubmed
	Sheng J, Price D, Koliatsos V. Disruption of corticocortical connections ameliorates amyloid burden in terminal fields in a transgenic model of Abeta amyloidosis. J Neurosci. 2002;22:9794-9 pubmed
	Choi Y, Park G, Kim T, Sunwoo I, Steinert P, Kim S. Sporadic inclusion body myositis correlates with increased expression and cross-linking by transglutaminases 1 and 2. J Biol Chem. 2000;275:8703-10 pubmed

product information

Product Type :

Antibody

Product Name :

Amyloid Precursor Protein Monoclonal Antibody (LN27)

Catalog # :

13-0200

Quantity :

100 µg

Price :

US 482

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 0.1-0.5 µg/mL, Immunohistochemistry: 5-10 µg/mL, Western Blot: 1:250

Species :

Human

Clone :

LN27

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

Amyloid Precursor Protein (APP) or Amyloid beta precursor protein functions as a cell surface kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. APP is important for neurite growth, neuronal adhesion and axonogenesis. APP is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms resulting from alternative splicing. Proteolytic cleavage of APP by beta- and gamma-secretases results in the generation of beta amyloid, which is the primary component of senile plaques. Senile plaques are one of the major histopathologic features of Alzheimer's disease. Abnormal regulation and processing of APP also plays a role in Down's syndrome, early onset familial Alzheimer's disease, and cerebral hemorrhage.

Immunogen :

Rec-beta-amyloid precursor protein (beta-APP 695)

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-0.5 µg/mL, Immunohistochemistry: 5-10 µg/mL, Western Blot: 1:250

Aliases :

A4; AAA; ABETA; Abeta40; Abeta42; ABPP; AD1; Adap; AG; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alpha-CTF; Alpha-secretase C-terminal fragment; Alzheimer disease amyloid A4 protein homolog; alzheimer disease amyloid protein; amyloid A4; Amyloid b precursor protein; amyloid beta (A4) precursor protein; amyloid beta A4 protein; amyloid beta precursor protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; Amyloid precursor protein; Amyloid ß precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; Amyloid-beta protein 40; Amyloid-beta protein 42; Amyloidogenic glycoprotein; APP; APP-C57; APP-C59; APP-C99; APPI; appican; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; betaApp; Beta-APP40; Beta-APP42; Beta-CTF; Beta-secretase C-terminal fragment; C31; C80; C83; C99; Cerebral vascular amyloid peptide; CTFgamma; CVAP; E030013M08Rik; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); Gamma-secretase C-terminal fragment 50; Gamma-secretase C-terminal fragment 57; Gamma-secretase C-terminal fragment 59; N-APP; P3(40); P3(42); Pan-Abeta; peptidase nexin-II; PN2; PN-II; PreA4; protease nexin II; protease nexin-II; S-APP-alpha; S-APP-beta; Soluble APP-alpha; Soluble APP-beta; testicular tissue protein Li 2

more info or order :

Invitrogen product webpage