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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD279 (PD-1) Monoclonal Antibody (J43), PE, eBioscience™
catalog :
12-9985-82
quantity :
100 µg
price :
US 287
clonality :
monoclonal
host :
hamsters
conjugate :
PE
clone name :
J43
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 73
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 5d
Ma Z, Zhang W, Dong B, Xin Z, Ji Y, Su R, et al. Docetaxel remodels prostate cancer immune microenvironment and enhances checkpoint inhibitor-based immunotherapy. Theranostics. 2022;12:4965-4979 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 1c
Sun Z, Yao Y, You M, Liu J, Guo W, Qi Z, et al. The kinase PDK1 is critical for promoting T follicular helper cell differentiation. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6e
Hajaj E, Eisenberg G, Klein S, Frankenburg S, Merims S, Ben David I, et al. SLAMF6​ deficiency augments tumor killing and skews toward an effector phenotype revealing it as a novel T cell checkpoint. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:100; fig e8a
Canon J, Rex K, Saiki A, Mohr C, Cooke K, Bagal D, et al. The clinical KRAS(G12C) inhibitor AMG 510 drives anti-tumour immunity. Nature. 2019;575:217-223 pubmed publisher
  • flow cytometry; mouse; fig 3
Van Der Werf N, Redpath S, Azuma M, Yagita H, Taylor M. Th2 cell-intrinsic hypo-responsiveness determines susceptibility to helminth infection. PLoS Pathog. 2013;9:e1003215 pubmed publisher
  • flow cytometry; mouse; fig 1
Tousif S, Singh Y, Prasad D, Sharma P, Van Kaer L, Das G. T cells from Programmed Death-1 deficient mice respond poorly to Mycobacterium tuberculosis infection. PLoS ONE. 2011;6:e19864 pubmed publisher
Takele Y, Mulaw T, Adem E, Womersley R, Kaforou M, Franssen S, et al. Recurrent visceral leishmaniasis relapses in HIV co-infected patients are characterized by less efficient immune responses and higher parasite load. iScience. 2023;26:105867 pubmed publisher
Li Q, Zhang L, You W, Xu J, Dai J, Hua D, et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun. 2022;13:7677 pubmed publisher
Hu J, He J, Wang Y, Zhao Y, Fang K, Dong Y, et al. Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy. J Immunother Cancer. 2022;10: pubmed publisher
Takele Y, Mulaw T, Adem E, Shaw C, Franssen S, Womersley R, et al. Immunological factors, but not clinical features, predict visceral leishmaniasis relapse in patients co-infected with HIV. Cell Rep Med. 2022;3:100487 pubmed publisher
Sato Y, Oguchi A, Fukushima Y, Masuda K, Toriu N, Taniguchi K, et al. CD153/CD30 signaling promotes age-dependent tertiary lymphoid tissue expansion and kidney injury. J Clin Invest. 2022;132: pubmed publisher
Wilfahrt D, Philips R, Lama J, Kizerwetter M, Shapiro M, McCue S, et al. Histone deacetylase 3 represses cholesterol efflux during CD4+ T-cell activation. elife. 2021;10: pubmed publisher
Kalim K, Yang J, Modur V, Nguyen P, Li Y, Zheng Y, et al. Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity. Front Immunol. 2021;12:726393 pubmed publisher
Robles Valero J, Fernandez Nevado L, Lorenzo Martín L, Cuadrado M, Fernández Pisonero I, Rodríguez Fdez S, et al. Cancer-associated mutations in VAV1 trigger variegated signaling outputs and T-cell lymphomagenesis. EMBO J. 2021;40:e108125 pubmed publisher
Sun Y, Lu D, Yin Y, Song J, Liu Y, Hao W, et al. PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer. Nat Commun. 2021;12:5147 pubmed publisher
Yang Z, Xu G, Wang B, Liu Y, Zhang L, Jing T, et al. USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade. Nat Commun. 2021;12:4852 pubmed publisher
Zhou X, Zhu X, Li C, Li Y, Ye Z, Shapiro V, et al. Stearoyl-CoA Desaturase-Mediated Monounsaturated Fatty Acid Availability Supports Humoral Immunity. Cell Rep. 2021;34:108601 pubmed publisher
Gu M, Zhou X, Sohn J, Zhu L, Jie Z, Yang J, et al. NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity. Nat Immunol. 2021;22:193-204 pubmed publisher
Ferrere G, Tidjani Alou M, Liu P, Goubet A, Fidelle M, Kepp O, et al. Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade. JCI Insight. 2021;6: pubmed publisher
Nguyen H, Ticer T, Bastian D, Kuril S, Li H, Du H, et al. Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease. Cell Rep. 2020;33:108316 pubmed publisher
Lee H, Park J, Yoo H, Lee H, Lee B, Kim J. The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses. Antioxidants (Basel). 2020;9: pubmed publisher
Marinaik C, Kingstad Bakke B, Lee W, Hatta M, Sonsalla M, Larsen A, et al. Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants. Cell Rep Med. 2020;1:100095 pubmed publisher
Fumagalli V, Di Lucia P, Venzin V, Bono E, Jordan R, Frey C, et al. Serum HBsAg clearance has minimal impact on CD8+ T cell responses in mouse models of HBV infection. J Exp Med. 2020;217: pubmed publisher
Liu W, Dai E, Liu Z, Ma C, Guo Z, Bartlett D. In Situ Therapeutic Cancer Vaccination with an Oncolytic Virus Expressing Membrane-Tethered IL-2. Mol Ther Oncolytics. 2020;17:350-360 pubmed publisher
Shang K, Wang Z, Hu Y, Huang Y, Yuan K, Yu Y. Gene silencing of indoleamine 2,3-dioxygenase 1 inhibits lung cancer growth by suppressing T-cell exhaustion. Oncol Lett. 2020;19:3827-3838 pubmed publisher
Doo D, Meza Perez S, Londoño A, Goldsberry W, Katre A, Boone J, et al. Inhibition of the Wnt/β-catenin pathway enhances antitumor immunity in ovarian cancer. Ther Adv Med Oncol. 2020;12:1758835920913798 pubmed publisher
Ebelt N, Zuniga E, Johnson B, Diamond D, Manuel E. 5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma. Front Immunol. 2020;11:538 pubmed publisher
Goldsberry W, Meza Perez S, Londoño A, Katre A, Mott B, Roane B, et al. Inhibiting WNT Ligand Production for Improved Immune Recognition in the Ovarian Tumor Microenvironment. Cancers (Basel). 2020;12: pubmed publisher
Sato R, Makino Okamura C, Lin Q, Wang M, Shoemaker J, Kurosaki T, et al. Repurposing the psoriasis drug Oxarol to an ointment adjuvant for the influenza vaccine. Int Immunol. 2020;32:499-507 pubmed publisher
Mohebiany A, Ramphal N, Karram K, Di Liberto G, Novkovic T, Klein M, et al. Microglial A20 Protects the Brain from CD8 T-Cell-Mediated Immunopathology. Cell Rep. 2020;30:1585-1597.e6 pubmed publisher
Chen L, Yang Q, Li Y, Yang L, Liu J, Li H, et al. Targeting CMTM6 Suppresses Stem Cell-Like Properties and Enhances Antitumor Immunity in Head and Neck Squamous Cell Carcinoma. Cancer Immunol Res. 2020;8:179-191 pubmed publisher
Gao C, Zhang M, Song Q, Dong J. PD-1 inhibitors dependent CD8+ T cells inhibit mouse colon cancer cell metastasis. Onco Targets Ther. 2019;12:6961-6971 pubmed publisher
Burrack A, Spartz E, Raynor J, Wang I, Olson M, Stromnes I. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019;28:2140-2155.e6 pubmed publisher
Liu T, Yang Q, Cao Y, Yuan W, Lei A, Zhou P, et al. Cyclooxygenase-1 Regulates the Development of Follicular Th Cells via Prostaglandin E2. J Immunol. 2019;203:864-872 pubmed publisher
Zhang H, Chen C, He J, Zhang J, Liu Z, Sun T. Anti-PD-1 Antibody Administration following Hip Fracture Surgery Reverses Immune Dysfunction and Decreases Susceptibility to Infection. Mediators Inflamm. 2019;2019:8492090 pubmed publisher
Rocamora Reverte L, Tuzlak S, von Raffay L, Tisch M, Fiegl H, Drach M, et al. Glucocorticoid Receptor-Deficient Foxp3+ Regulatory T Cells Fail to Control Experimental Inflammatory Bowel Disease. Front Immunol. 2019;10:472 pubmed publisher
Cheng Y, Zhu X, Wang X, Zhuang Q, Huyan X, Sun X, et al. Trichinella spiralis Infection Mitigates Collagen-Induced Arthritis via Programmed Death 1-Mediated Immunomodulation. Front Immunol. 2018;9:1566 pubmed publisher
Zhang Y, Tech L, George L, Acs A, Durrett R, Hess H, et al. Plasma cell output from germinal centers is regulated by signals from Tfh and stromal cells. J Exp Med. 2018;215:1227-1243 pubmed publisher
Schager A, Dominguez Medina C, Necchi F, Micoli F, Goh Y, Goodall M, et al. IgG Responses to Porins and Lipopolysaccharide within an Outer Membrane-Based Vaccine against Nontyphoidal Salmonella Develop at Discordant Rates. MBio. 2018;9: pubmed publisher
Sharma M, Rodriguez P, Koehn B, Baban B, Cui Y, Guo G, et al. Activation of p53 in Immature Myeloid Precursor Cells Controls Differentiation into Ly6c+CD103+ Monocytic Antigen-Presenting Cells in Tumors. Immunity. 2018;48:91-106.e6 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Kwak J, Laskowski J, Li H, McSharry M, Sippel T, Bullock B, et al. Complement Activation via a C3a Receptor Pathway Alters CD4+ T Lymphocytes and Mediates Lung Cancer Progression. Cancer Res. 2018;78:143-156 pubmed publisher
Zhu Y, Herndon J, Sojka D, Kim K, Knolhoff B, Zuo C, et al. Tissue-Resident Macrophages in Pancreatic Ductal Adenocarcinoma Originate from Embryonic Hematopoiesis and Promote Tumor Progression. Immunity. 2017;47:323-338.e6 pubmed publisher
Peer S, Baier G, Gruber T. Cblb-deficient T cells are less susceptible to PD-L1-mediated inhibition. Oncotarget. 2017;8:41841-41853 pubmed publisher
Jiang H, Rivera Molina Y, Gomez Manzano C, Clise Dwyer K, Bover L, Vence L, et al. Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination. Cancer Res. 2017;77:3894-3907 pubmed publisher
Fabian K, Chi Sabins N, Taylor J, Fecek R, Weinstein A, Storkus W. Therapeutic efficacy of combined vaccination against tumor pericyte-associated antigens DLK1 and DLK2 in mice. Oncoimmunology. 2017;6:e1290035 pubmed publisher
Zamarin D, Holmgaard R, Ricca J, Plitt T, Palese P, Sharma P, et al. Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity. Nat Commun. 2017;8:14340 pubmed publisher
Sonner J, Deumelandt K, Ott M, Thomé C, Rauschenbach K, Schulz S, et al. The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas. Oncoimmunology. 2016;5:e1240858 pubmed publisher
Chen L, Yi X, Goswami S, Ahn Y, Roybal J, Yang Y, et al. Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression. Oncoimmunology. 2016;5:e1234570 pubmed publisher
Kroon P, Gadiot J, Peeters M, Gasparini A, Deken M, Yagita H, et al. Concomitant targeting of programmed death-1 (PD-1) and CD137 improves the efficacy of radiotherapy in a mouse model of human BRAFV600-mutant melanoma. Cancer Immunol Immunother. 2016;65:753-63 pubmed publisher
Tian Y, Kuo C, Akbari O, Ou J. Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission. Immunity. 2016;44:1204-14 pubmed publisher
Sebastian M, Lopez Ocasio M, Metidji A, RIEDER S, Shevach E, Thornton A. Helios Controls a Limited Subset of Regulatory T Cell Functions. J Immunol. 2016;196:144-55 pubmed publisher
Chen L, Gibbons D, Goswami S, Cortez M, Ahn Y, Byers L, et al. Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression. Nat Commun. 2014;5:5241 pubmed publisher
Arrevillaga Boni G, Hernandez Ruiz M, Castillo E, Ortiz Navarrete V. Intercellular communication through contacts between continuous pseudopodial extensions in a macrophage-like cell line. Cell Commun Adhes. 2014;21:213-20 pubmed publisher
Dey M, Chang A, Wainwright D, Ahmed A, Han Y, Balyasnikova I, et al. Heme oxygenase-1 protects regulatory T cells from hypoxia-induced cellular stress in an experimental mouse brain tumor model. J Neuroimmunol. 2014;266:33-42 pubmed publisher
Shepard M, Bonney E. PD-1 regulates T cell proliferation in a tissue and subset-specific manner during normal mouse pregnancy. Immunol Invest. 2013;42:385-408 pubmed publisher
Chang K, Burnham C, Compton S, Rasche D, Mazuski R, McDonough J, et al. Blockade of the negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis. Crit Care. 2013;17:R85 pubmed publisher
Todd E, Deady L, Morley S. Intrinsic T- and B-cell defects impair T-cell-dependent antibody responses in mice lacking the actin-bundling protein L-plastin. Eur J Immunol. 2013;43:1735-44 pubmed publisher
Dorfmeier C, Tzvetkov E, Gatt A, McGettigan J. Investigating the role for IL-21 in rabies virus vaccine-induced immunity. PLoS Negl Trop Dis. 2013;7:e2129 pubmed publisher
Veiga Parga T, Suryawanshi A, Mulik S, Giménez F, Sharma S, Sparwasser T, et al. On the role of regulatory T cells during viral-induced inflammatory lesions. J Immunol. 2012;189:5924-33 pubmed publisher
Li L, Huang L, Ye H, Song S, Bajwa A, Lee S, et al. Dendritic cells tolerized with adenosine A?AR agonist attenuate acute kidney injury. J Clin Invest. 2012;122:3931-42 pubmed publisher
Baru A, Untucht C, Ganesh V, Hesse C, Mayer C, Sparwasser T. Optimal isolation of functional Foxp3+ induced regulatory T cells using DEREG mice. PLoS ONE. 2012;7:e44760 pubmed publisher
Yokosuka T, Takamatsu M, Kobayashi Imanishi W, Hashimoto Tane A, Azuma M, Saito T. Programmed cell death 1 forms negative costimulatory microclusters that directly inhibit T cell receptor signaling by recruiting phosphatase SHP2. J Exp Med. 2012;209:1201-17 pubmed publisher
de Bruin A, Libregts S, Valkhof M, Boon L, Touw I, Nolte M. IFN? induces monopoiesis and inhibits neutrophil development during inflammation. Blood. 2012;119:1543-54 pubmed publisher
Monaghan S, Thakkar R, Heffernan D, Huang X, Chung C, Lomas Neira J, et al. Mechanisms of indirect acute lung injury: a novel role for the coinhibitory receptor, programmed death-1. Ann Surg. 2012;255:158-64 pubmed publisher
Serre K, Bénézech C, Desanti G, Bobat S, Toellner K, Bird R, et al. Helios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression. PLoS ONE. 2011;6:e20731 pubmed publisher
Zhang Y, Zhou Y, Lou J, Li J, Bo L, Zhu K, et al. PD-L1 blockade improves survival in experimental sepsis by inhibiting lymphocyte apoptosis and reversing monocyte dysfunction. Crit Care. 2010;14:R220 pubmed publisher
Lages C, Lewkowich I, Sproles A, Wills Karp M, Chougnet C. Partial restoration of T-cell function in aged mice by in vitro blockade of the PD-1/?PD-L1 pathway. Aging Cell. 2010;9:785-98 pubmed publisher
Ishiwata K, Watanabe N, Guo M, Tomihara K, Brumlik M, Yagita H, et al. Costimulator B7-DC attenuates strong Th2 responses induced by Nippostrongylus brasiliensis. J Immunol. 2010;184:2086-94 pubmed publisher
Taglauer E, Yankee T, Petroff M. Maternal PD-1 regulates accumulation of fetal antigen-specific CD8+ T cells in pregnancy. J Reprod Immunol. 2009;80:12-21 pubmed publisher
Webster K, Walters S, Kohler R, Mrkvan T, Boyman O, Surh C, et al. In vivo expansion of T reg cells with IL-2-mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. J Exp Med. 2009;206:751-60 pubmed publisher
Lucas J, Menke J, Rabacal W, Schoen F, Sharpe A, Kelley V. Programmed death ligand 1 regulates a critical checkpoint for autoimmune myocarditis and pneumonitis in MRL mice. J Immunol. 2008;181:2513-21 pubmed
Ansari M, Salama A, Chitnis T, Smith R, Yagita H, Akiba H, et al. The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice. J Exp Med. 2003;198:63-9 pubmed
product information
Product Type :
Antibody
Product Name :
CD279 (PD-1) Monoclonal Antibody (J43), PE, eBioscience™
Catalog # :
12-9985-82
Quantity :
100 µg
Price :
US 287
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Armenian Hamster
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
J43
Isotype :
IgG
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; Protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA