Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), PE, eBioscience | Invitrogen 12-9865-42 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), PE, eBioscience

catalog :

12-9865-42

quantity :

100 Tests

price :

US 394.00

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

CR55T33

reactivity :

human, mouse

application :

flow cytometry

citations: 6

Reference
Biega x142 a x, Gajek A, Marczak A, Rogalska A. Olaparib-Resistant BRCA2MUT Ovarian Cancer Cells with Restored BRCA2 Abrogate Olaparib-Induced DNA Damage and G2/M Arrest Controlled by the ATR/CHK1 Pathway for Survival. Cells. 2023;12: pubmed publisher
Wang Y, Lu M, Li Y, Tang H, Hsiao P, Chen B, et al. Microevolution of CG23-I Hypervirulent Klebsiella pneumoniae during Recurrent Infections in a Single Patient. Microbiol Spectr. 2022;10:e0207722 pubmed publisher
Lee M, Geitgey D, Hamilton J, Boss J, Scharer C, Spangle J, et al. Adipocyte-mediated epigenomic instability in human T-ALL cells is cytotoxic and phenocopied by epigenetic-modifying drugs. Front Cell Dev Biol. 2022;10:909557 pubmed publisher
Moens S, Zhao P, Baietti M, Marinelli O, Van Haver D, Impens F, et al. The mitotic checkpoint is a targetable vulnerability of carboplatin-resistant triple negative breast cancers. Sci Rep. 2021;11:3176 pubmed publisher
Tan D, Li Y, Yang C, Li J, Tan S, Chin D, et al. PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells. Cell Rep. 2019;26:2316-2328.e6 pubmed publisher
Lang P, Nanjangud G, Sokolsky Papkov M, Shaw C, Hwang D, Parker J, et al. ATR maintains chromosomal integrity during postnatal cerebellar neurogenesis and is required for medulloblastoma formation. Development. 2016;143:4038-4052 pubmed

product information

Product Type :

Antibody

Product Name :

Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), PE, eBioscience

Catalog # :

12-9865-42

Quantity :

100 Tests

Price :

US 394.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 5 uL (0.25 ug)/test

Species :

Human, Mouse

Clone :

CR55T33

Isotype :

IgG1, kappa

Storage :

4 C, store in dark, DO NOT FREEZE!

Description :

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 5 uL (0.25 ug)/test

Aliases :

AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329