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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Perforin Monoclonal Antibody (eBioOMAK-D), PE, eBioscience
catalog :
12-9392-82
quantity :
100 µg
price :
US 377
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
eBioOMAK-D
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 59
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig s4b
Souza C, Ketelut Carneiro N, Milanezi C, Faccioli L, Gardinassi L, Silva J. NLRC4 inhibits NLRP3 inflammasome and abrogates effective antifungal CD8+ T cell responses. iScience. 2021;24:102548 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...
Piersma S, Poursine Laurent J, Yang L, Barber G, Parikh B, Yokoyama W. Virus infection is controlled by hematopoietic and stromal cell sensing of murine cytomegalovirus through STING. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:66; loading ...; fig 2c
Wang F, Meng M, Mo B, Yang Y, Ji Y, Huang P, et al. Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. Nat Commun. 2018;9:4874 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4j
Förster M, Boora R, Petrov J, Fodil N, Albanese I, Kim J, et al. A role for the histone H2A deubiquitinase MYSM1 in maintenance of CD8+ T cells. Immunology. 2017;151:110-121 pubmed publisher
  • flow cytometry; mouse; fig 3
Zou Y, Chen T, Han M, Wang H, Yan W, Song G, et al. Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure. J Immunol. 2010;184:466-75 pubmed publisher
Lin Q, Fang X, Liang G, Luo Q, Cen Y, Shi Y, et al. Silencing CTNND1 Mediates Triple-Negative Breast Cancer Bone Metastasis via Upregulating CXCR4/CXCL12 Axis and Neutrophils Infiltration in Bone. Cancers (Basel). 2021;13: pubmed publisher
Li J, Wang S, Wang N, Zheng Y, Yang B, Wang X, et al. Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration. Cell Commun Signal. 2021;19:89 pubmed publisher
Wu M, Xia X, Hu J, Fowlkes N, Li S. WSX1 act as a tumor suppressor in hepatocellular carcinoma by downregulating neoplastic PD-L1 expression. Nat Commun. 2021;12:3500 pubmed publisher
Silva Cayetano A, Foster W, Innocentin S, Belij Rammerstorfer S, Spencer A, Burton O, et al. A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice. Med (N Y). 2021;2:243-262.e8 pubmed publisher
Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, et al. PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell Dysfunction. Cell Rep. 2020;33:108278 pubmed publisher
Wu B, Chiang H, Sun X, Yuan B, Mitra P, Hu Y, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8: pubmed publisher
Hu M, Lu Y, Qi Y, Zhang Z, Wang S, Xu Y, et al. SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells. Cancer Immunol Res. 2020;8:1150-1162 pubmed publisher
Fan Z, Tian Y, Chen Z, Liu L, Zhou Q, He J, et al. Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors. EMBO Mol Med. 2020;12:e11571 pubmed publisher
Shi L, Goswami S, Fu T, Guan B, Chen J, Xiong L, et al. Blockade of CTLA-4 and PD-1 Enhances Adoptive T-cell Therapy Efficacy in an ICOS-Mediated Manner. Cancer Immunol Res. 2019;7:1803-1812 pubmed publisher
Mondragon L, Mhaidly R, De Donatis G, Tosolini M, Dao P, Martin A, et al. GAPDH Overexpression in the T Cell Lineage Promotes Angioimmunoblastic T Cell Lymphoma through an NF-κB-Dependent Mechanism. Cancer Cell. 2019;36:268-287.e10 pubmed publisher
Xu W, Cherrier D, Chea S, Vosshenrich C, Serafini N, Petit M, et al. An Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials. Immunity. 2019;50:1054-1068.e3 pubmed publisher
Wu J, Zhang H, Shi X, Xiao X, Fan Y, Minze L, et al. Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4+ T Cell Dysfunction. Immunity. 2017;47:1114-1128.e6 pubmed publisher
Klein Hessling S, Muhammad K, Klein M, Pusch T, Rudolf R, Flöter J, et al. NFATc1 controls the cytotoxicity of CD8+ T cells. Nat Commun. 2017;8:511 pubmed publisher
YEKU O, Purdon T, Koneru M, Spriggs D, Brentjens R. Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment. Sci Rep. 2017;7:10541 pubmed publisher
Kim K, Bae S, Capece T, Nedelkovska H, de Rubio R, Smrcka A, et al. Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment. Nat Commun. 2017;8:15365 pubmed publisher
Dai K, Huang L, Huang Y, Chen Z, Yang L, Jiang Y. 1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8+ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model. Front Immunol. 2017;8:320 pubmed publisher
Clever D, Roychoudhuri R, Constantinides M, Askenase M, Sukumar M, Klebanoff C, et al. Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche. Cell. 2016;166:1117-1131.e14 pubmed publisher
Solaymani Mohammadi S, Lakhdari O, Minev I, Shenouda S, Frey B, Billeskov R, et al. Lack of the programmed death-1 receptor renders host susceptible to enteric microbial infection through impairing the production of the mucosal natural killer cell effector molecules. J Leukoc Biol. 2016;99:475-82 pubmed publisher
Concepcion A, Salas J, Sáez E, Sarvide S, Ferrer A, Portu A, et al. CD8+ T cells undergo activation and programmed death-1 repression in the liver of aged Ae2a,b-/- mice favoring autoimmune cholangitis. Oncotarget. 2015;6:28588-606 pubmed publisher
Krneta T, Gillgrass A, Chew M, Ashkar A. The breast tumor microenvironment alters the phenotype and function of natural killer cells. Cell Mol Immunol. 2016;13:628-39 pubmed publisher
Guo Y, Luan L, Rabacal W, Bohannon J, Fensterheim B, Hernandez A, et al. IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-γ. J Immunol. 2015;195:2353-64 pubmed publisher
Hou H, Liu W, Wu S, Lu Y, Peng J, Zhu Y, et al. Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock. PLoS ONE. 2014;9:e110585 pubmed publisher
Lee S, Park H, Park S, Kim N, Hong S. Immunomodulatory effect of poly-?-glutamic acid derived from Bacillus subtilis on natural killer dendritic cells. Biochem Biophys Res Commun. 2014;443:413-21 pubmed publisher
Murphy K, Erickson J, Johnson C, Seiler C, Bedi J, Hu P, et al. CD8+ T cell-independent tumor regression induced by Fc-OX40L and therapeutic vaccination in a mouse model of glioma. J Immunol. 2014;192:224-33 pubmed publisher
Takigawa T, Miyazaki H, Kinoshita M, Kawarabayashi N, Nishiyama K, Hatsuse K, et al. Glucocorticoid receptor-dependent immunomodulatory effect of ursodeoxycholic acid on liver lymphocytes in mice. Am J Physiol Gastrointest Liver Physiol. 2013;305:G427-38 pubmed publisher
Liu R, Engels B, Schreiber K, Ciszewski C, Schietinger A, Schreiber H, et al. IL-15 in tumor microenvironment causes rejection of large established tumors by T cells in a noncognate T cell receptor-dependent manner. Proc Natl Acad Sci U S A. 2013;110:8158-63 pubmed publisher
Stehle F, Schulz K, Fahldieck C, Kalich J, Lichtenfels R, Riemann D, et al. Reduced immunosuppressive properties of axitinib in comparison with other tyrosine kinase inhibitors. J Biol Chem. 2013;288:16334-47 pubmed publisher
Riese M, Wang L, Moon E, Joshi R, Ranganathan A, June C, et al. Enhanced effector responses in activated CD8+ T cells deficient in diacylglycerol kinases. Cancer Res. 2013;73:3566-77 pubmed publisher
Peng H, Sun R, Tang L, Wei H, Tian Z. CD62L is critical for maturation and accumulation of murine hepatic NK cells in response to viral infection. J Immunol. 2013;190:4255-62 pubmed publisher
Ali R, Naqvi R, Kumar S, Bhat A, Rao D. Multiple antigen peptide containing B and T cell epitopes of F1 antigen of Yersinia pestis showed enhanced Th1 immune response in murine model. Scand J Immunol. 2013;77:361-71 pubmed publisher
Ma D, Suthar M, Kasahara S, Gale M, Clark E. CD22 is required for protection against West Nile virus Infection. J Virol. 2013;87:3361-75 pubmed publisher
Fullerton A, Roth R, Ganey P. 2,3,7,8-TCDD enhances the sensitivity of mice to concanavalin A immune-mediated liver injury. Toxicol Appl Pharmacol. 2013;266:317-27 pubmed publisher
Bao Y, Zheng J, Han C, Jin J, Han H, Liu Y, et al. Tyrosine kinase Btk is required for NK cell activation. J Biol Chem. 2012;287:23769-78 pubmed publisher
Wu L, Yun Z, Tagawa T, Rey McIntyre K, de Perrot M. CTLA-4 blockade expands infiltrating T cells and inhibits cancer cell repopulation during the intervals of chemotherapy in murine mesothelioma. Mol Cancer Ther. 2012;11:1809-19 pubmed publisher
Wu X, Chen Y, Wei H, Sun R, Tian Z. Development of murine hepatic NK cells during ontogeny: comparison with spleen NK cells. Clin Dev Immunol. 2012;2012:759765 pubmed publisher
Hao J, Dong S, Xia S, He W, Jia H, Zhang S, et al. Regulatory role of V?1 ?? T cells in tumor immunity through IL-4 production. J Immunol. 2011;187:4979-86 pubmed publisher
Yamashita J, Iwamura C, Mitsumori K, Hosokawa H, Sasaki T, Takahashi M, et al. Murine Schnurri-2 controls natural killer cell function and lymphoma development. Leuk Lymphoma. 2012;53:479-86 pubmed publisher
Kitajima M, Ito T, Tumes D, Endo Y, Onodera A, Hashimoto K, et al. Memory type 2 helper T cells induce long-lasting antitumor immunity by activating natural killer cells. Cancer Res. 2011;71:4790-8 pubmed publisher
Narumi K, Udagawa T, Kondoh A, Kobayashi A, Hara H, Ikarashi Y, et al. In vivo delivery of interferon-? gene enhances tumor immunity and suppresses immunotolerance in reconstituted lymphopenic hosts. Gene Ther. 2012;19:34-48 pubmed publisher
Tosiek M, Gruber A, Bader S, Mauel S, Hoymann H, Prettin S, et al. CD4+CD25+Foxp3+ regulatory T cells are dispensable for controlling CD8+ T cell-mediated lung inflammation. J Immunol. 2011;186:6106-18 pubmed publisher
Cavassani K, Carson W, Moreira A, Wen H, Schaller M, Ishii M, et al. The post sepsis-induced expansion and enhanced function of regulatory T cells create an environment to potentiate tumor growth. Blood. 2010;115:4403-11 pubmed publisher
Lai Y, Lin C, Liao W, Tang C, Chen S. CD4+ T cell-derived IL-2 signals during early priming advances primary CD8+ T cell responses. PLoS ONE. 2009;4:e7766 pubmed publisher
Thapa M, Carr D. CXCR3 deficiency increases susceptibility to genital herpes simplex virus type 2 infection: Uncoupling of CD8+ T-cell effector function but not migration. J Virol. 2009;83:9486-501 pubmed publisher
Xie X, Stadnisky M, Brown M. MHC class I Dk locus and Ly49G2+ NK cells confer H-2k resistance to murine cytomegalovirus. J Immunol. 2009;182:7163-71 pubmed publisher
Kassim S, Rajasagi N, Ritz B, Pruett S, Gardner E, Chervenak R, et al. Dendritic cells are required for optimal activation of natural killer functions following primary infection with herpes simplex virus type 1. J Virol. 2009;83:3175-86 pubmed publisher
Saito K, Torii M, Ma N, Tsuchiya T, Wang L, Hori T, et al. Differential regulatory function of resting and preactivated allergen-specific CD4+ CD25+ regulatory T cells in Th2-type airway inflammation. J Immunol. 2008;181:6889-97 pubmed
Porrett P, Lee M, Lian M, Wang J, Caton A, Deng S, et al. A direct comparison of rejection by CD8 and CD4 T cells in a transgenic model of allotransplantation. Arch Immunol Ther Exp (Warsz). 2008;56:193-200 pubmed publisher
Jin G, Hirano T, Murakami M. Combination treatment with IL-2 and anti-IL-2 mAbs reduces tumor metastasis via NK cell activation. Int Immunol. 2008;20:783-9 pubmed publisher
Murakami T, Sato A, Chun N, Hara M, Naito Y, Kobayashi Y, et al. Transcriptional modulation using HDACi depsipeptide promotes immune cell-mediated tumor destruction of murine B16 melanoma. J Invest Dermatol. 2008;128:1506-16 pubmed publisher
Strid J, Roberts S, Filler R, Lewis J, Kwong B, Schpero W, et al. Acute upregulation of an NKG2D ligand promotes rapid reorganization of a local immune compartment with pleiotropic effects on carcinogenesis. Nat Immunol. 2008;9:146-54 pubmed publisher
Hutchens M, Luker K, Sottile P, Sonstein J, Lukacs N, Nunez G, et al. TLR3 increases disease morbidity and mortality from vaccinia infection. J Immunol. 2008;180:483-91 pubmed
Gu T, Kilinc M, Egilmez N. Transient activation of tumor-associated T-effector/memory cells promotes tumor eradication via NK-cell recruitment: minimal role for long-term T-cell immunity in cure of metastatic disease. Cancer Immunol Immunother. 2008;57:997-1005 pubmed
Fehniger T, Cai S, Cao X, Bredemeyer A, Presti R, French A, et al. Acquisition of murine NK cell cytotoxicity requires the translation of a pre-existing pool of granzyme B and perforin mRNAs. Immunity. 2007;26:798-811 pubmed
Chaitra M, Shaila M, Nayak R. Evaluation of T-cell responses to peptides with MHC class I-binding motifs derived from PE_PGRS 33 protein of Mycobacterium tuberculosis. J Med Microbiol. 2007;56:466-74 pubmed
product information
Product Type :
Antibody
Product Name :
Perforin Monoclonal Antibody (eBioOMAK-D), PE, eBioscience
Catalog # :
12-9392-82
Quantity :
100 µg
Price :
US 377
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 1 µg/test
Species :
Mouse
Clone :
eBioOMAK-D
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test
Aliases :
Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1; Prf-1; PRF1 (pore forming protein 1); RATCYTA; RP11-710A11.3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA