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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Granzyme B Monoclonal Antibody (GB11), PE, eBioscience
catalog :
12-8899-41
quantity :
25 Tests
price :
US 169.00
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
GB11
reactivity :
human, dogs
application :
flow cytometry
more info or order :
citations: 59
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 4a
Liu G, Yu Y, Feng F, Zhu P, Zhang H, Zhang D, et al. Human CD8+CD28- T suppressor cells expanded by common gamma chain (γc) cytokines retain steady allospecific suppressive capacity in vivo. BMC Immunol. 2020;21:23 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Li N, van Unen V, Höllt T, Thompson A, van Bergen J, Pezzotti N, et al. Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine. J Exp Med. 2018;215:1383-1396 pubmed publisher
  • flow cytometry; human; loading ...; fig 3f
Su S, Liao J, Liu J, Huang D, He C, Chen F, et al. Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Res. 2017;27:461-482 pubmed publisher
  • flow cytometry; dogs
Grøndahl Rosado C, Bønsdorff T, Brun Hansen H, Storset A. NCR1+ cells in dogs show phenotypic characteristics of natural killer cells. Vet Res Commun. 2015;39:19-30 pubmed publisher
  • flow cytometry; human
Pegram H, Purdon T, van Leeuwen D, Curran K, Giralt S, Barker J, et al. IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia. Leukemia. 2015;29:415-22 pubmed publisher
Guo D, Jin C, Gao Y, Lin H, Zhang L, Zhou Y, et al. GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint. Cell Biosci. 2023;13:51 pubmed publisher
Yang C, Qian C, Zhang T, Zheng W, Zhang S, Wang S, et al. The killing effect of Tanshinol on breast cancer cells: insight into the reversion of TGF-β1-mediated suppression of NK cell functions. Front Biosci (Landmark Ed). 2021;26:1106-1118 pubmed publisher
Gao Y, Lin H, Guo D, Cheng S, Zhou Y, Zhang L, et al. Suppression of 4.1R enhances the potency of NKG2D-CAR T cells against pancreatic carcinoma via activating ERK signaling pathway. Oncogenesis. 2021;10:62 pubmed publisher
Alsaleh G, Panse I, Swadling L, Zhang H, Richter F, Meyer A, et al. Autophagy in T cells from aged donors is maintained by spermidine and correlates with function and vaccine responses. elife. 2020;9: pubmed publisher
Billiet L, Goetgeluk G, Bonte S, De Munter S, De Cock L, Pille M, et al. Human Thymic CD10+ PD-1+ Intraepithelial Lymphocyte Precursors Acquire Interleukin-15 Responsiveness at the CD1a- CD95+ CD28- CCR7- Developmental Stage. Int J Mol Sci. 2020;21: pubmed publisher
Grifoni A, Weiskopf D, Ramirez S, Mateus J, Dan J, Moderbacher C, et al. Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals. Cell. 2020;181:1489-1501.e15 pubmed publisher
Pontes Ferreira C, Cariste L, Ferri Moraschi B, Ferrarini Zanetti B, Won Han S, Araki Ribeiro D, et al. CXCR3 chemokine receptor guides Trypanosoma cruzi-specific T-cells triggered by DNA/adenovirus ASP2 vaccine to heart tissue after challenge. PLoS Negl Trop Dis. 2019;13:e0007597 pubmed publisher
Rotolo A, Caputo V, Holubova M, Baxan N, Dubois O, Chaudhry M, et al. Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting. Cancer Cell. 2018;34:596-610.e11 pubmed publisher
Wang C, Edilova M, Wagar L, Mujib S, Singer M, Bernard N, et al. Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy. J Immunol. 2018;200:558-564 pubmed publisher
Nada M, Wang H, Workalemahu G, Tanaka Y, Morita C. Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulation. J Immunother Cancer. 2017;5:9 pubmed publisher
Jeffery H, Jeffery L, Lutz P, Corrigan M, Webb G, Hirschfield G, et al. Low-dose interleukin-2 promotes STAT-5 phosphorylation, Treg survival and CTLA-4-dependent function in autoimmune liver diseases. Clin Exp Immunol. 2017;188:394-411 pubmed publisher
Wu X, Zhang H, Xing Q, Cui J, Li J, Li Y, et al. PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients. Br J Cancer. 2014;111:1391-9 pubmed publisher
Lee J, Yeh C, Jung C, Chen C, Du M, Yu H, et al. Skewed distribution of IL-7 receptor-?-expressing effector memory CD8+ T cells with distinct functional characteristics in oral squamous cell carcinoma. PLoS ONE. 2014;9:e85521 pubmed publisher
Joedicke J, Dietze K, Zelinskyy G, Dittmer U. The phenotype and activation status of regulatory T cells during Friend retrovirus infection. Virol Sin. 2014;29:48-60 pubmed publisher
Legat A, Speiser D, Pircher H, Zehn D, Fuertes Marraco S. Inhibitory Receptor Expression Depends More Dominantly on Differentiation and Activation than "Exhaustion" of Human CD8?T Cells. Front Immunol. 2013;4:455 pubmed publisher
Kim E, Gasper D, Lee S, Plisch E, Svaren J, Suresh M. Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice. J Immunol. 2014;192:985-95 pubmed publisher
Ho L, Yit P, Ng L, Linn Y, Zhao Y, Sun L, et al. The road to memory: an early rest for the long journey. J Immunol. 2013;191:5603-14 pubmed publisher
Cho J, Kim H, Kim K, Yang D, Surh C, Sprent J. Unique features of naive CD8+ T cell activation by IL-2. J Immunol. 2013;191:5559-73 pubmed publisher
Zumwalde N, Domae E, Mescher M, Shimizu Y. ICAM-1-dependent homotypic aggregates regulate CD8 T cell effector function and differentiation during T cell activation. J Immunol. 2013;191:3681-93 pubmed publisher
Ivarsson M, Loh L, Marquardt N, Kekäläinen E, Berglin L, Björkström N, et al. Differentiation and functional regulation of human fetal NK cells. J Clin Invest. 2013;123:3889-901 pubmed publisher
Nizzoli G, Krietsch J, Weick A, Steinfelder S, Facciotti F, Gruarin P, et al. Human CD1c+ dendritic cells secrete high levels of IL-12 and potently prime cytotoxic T-cell responses. Blood. 2013;122:932-42 pubmed publisher
Hu Z, Zhang W, Usherwood E. Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect. J Immunol. 2013;191:312-22 pubmed publisher
Manzke N, Akhmetzyanova I, Hasenkrug K, Trilling M, Zelinskyy G, Dittmer U. CD4+ T cells develop antiretroviral cytotoxic activity in the absence of regulatory T cells and CD8+ T cells. J Virol. 2013;87:6306-13 pubmed publisher
Le Huu D, Matsushita T, Jin G, Hamaguchi Y, Hasegawa M, Takehara K, et al. Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease. Blood. 2013;121:3274-83 pubmed publisher
Bratke K, Prieschenk C, Garbe K, Kuepper M, Lommatzsch M, Virchow J. Plasmacytoid dendritic cells in allergic asthma and the role of inhaled corticosteroid treatment. Clin Exp Allergy. 2013;43:312-21 pubmed publisher
Hotho D, Kreefft K, Groothuismink Z, Janssen H, de Knegt R, Boonstra A. Natural killer cell activity and function in chronic HCV-infected patients during peg interferon and ribavirin: early effects of active substance use. Antiviral Res. 2013;97:347-55 pubmed publisher
Heninger A, Theil A, Wilhelm C, Petzold C, Huebel N, Kretschmer K, et al. IL-7 abrogates suppressive activity of human CD4+CD25+FOXP3+ regulatory T cells and allows expansion of alloreactive and autoreactive T cells. J Immunol. 2012;189:5649-58 pubmed publisher
Smithey M, Li G, Venturi V, Davenport M, Nikolich Zugich J. Lifelong persistent viral infection alters the naive T cell pool, impairing CD8 T cell immunity in late life. J Immunol. 2012;189:5356-66 pubmed publisher
Shen H, Shao H, Chen X, Wu F, Wang H, Huang Z, et al. Identification of a novel HLA-A2-restricted mutated Survivin epitope and induction of specific anti-HCC CTLs that could effectively cross-recognize wild-type Survivin antigen. Cancer Immunol Immunother. 2013;62:393-403 pubmed publisher
Chang P, Lee S, Hu X, Davey G, Duan G, Cho J, et al. Breakdown in repression of IFN-? mRNA leads to accumulation of self-reactive effector CD8+ T cells. J Immunol. 2012;189:701-10 pubmed publisher
Bao Y, Zheng J, Han C, Jin J, Han H, Liu Y, et al. Tyrosine kinase Btk is required for NK cell activation. J Biol Chem. 2012;287:23769-78 pubmed publisher
McNamara J, Schramm C, Singh A, Secor E, Guernsey L, Lefrancois L, et al. Phenotypic changes to the endogenous antigen-specific CD8+ T cell response correlates with the development and resolution of allergic airway disease. Am J Pathol. 2012;180:1991-2000 pubmed publisher
Garbe K, Bratke K, Wagner S, Virchow J, Lommatzsch M. Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age. Hum Immunol. 2012;73:493-7 pubmed publisher
Hippen K, Bucher C, Schirm D, Bearl A, Brender T, Mink K, et al. Blocking IL-21 signaling ameliorates xenogeneic GVHD induced by human lymphocytes. Blood. 2012;119:619-28 pubmed publisher
Tjin E, Konijnenberg D, Krebbers G, Mallo H, Drijfhout J, Franken K, et al. T-cell immune function in tumor, skin, and peripheral blood of advanced stage melanoma patients: implications for immunotherapy. Clin Cancer Res. 2011;17:5736-47 pubmed publisher
Frisullo G, Iorio R, Plantone D, Nociti V, Patanella A, Marti A, et al. Involvement of type I immune responses in swine-origin H1N1 influenza virus infection. Hum Immunol. 2011;72:632-5 pubmed publisher
Caccamo N, La Mendola C, Orlando V, Meraviglia S, Todaro M, Stassi G, et al. Differentiation, phenotype, and function of interleukin-17-producing human V?9V?2 T cells. Blood. 2011;118:129-38 pubmed publisher
Huntington N, Alves N, Legrand N, Lim A, Strick Marchand H, Mention J, et al. IL-15 transpresentation promotes both human T-cell reconstitution and T-cell-dependent antibody responses in vivo. Proc Natl Acad Sci U S A. 2011;108:6217-22 pubmed publisher
Zhou X, McElhaney J. Age-related changes in memory and effector T cells responding to influenza A/H3N2 and pandemic A/H1N1 strains in humans. Vaccine. 2011;29:2169-77 pubmed publisher
Kim H, Eyheramonho M, Pichavant M, Gonzalez Cambaceres C, Matangkasombut P, Cervio G, et al. A polymorphism in TIM1 is associated with susceptibility to severe hepatitis A virus infection in humans. J Clin Invest. 2011;121:1111-8 pubmed publisher
Libri V, Azevedo R, Jackson S, Di Mitri D, Lachmann R, Fuhrmann S, et al. Cytomegalovirus infection induces the accumulation of short-lived, multifunctional CD4+CD45RA+CD27+ T cells: the potential involvement of interleukin-7 in this process. Immunology. 2011;132:326-39 pubmed publisher
Kim E, Kim T, Kim N, Kim S, Kumar V, Lee K. Homotypic cell to cell cross-talk among human natural killer cells reveals differential and overlapping roles of 2B4 and CD2. J Biol Chem. 2010;285:41755-64 pubmed publisher
Marshall H, Prince A, Berg L, Welsh R. IFN-alpha beta and self-MHC divert CD8 T cells into a distinct differentiation pathway characterized by rapid acquisition of effector functions. J Immunol. 2010;185:1419-28 pubmed publisher
Elftman M, Hunzeker J, Mellinger J, Bonneau R, Norbury C, Truckenmiller M. Stress-induced glucocorticoids at the earliest stages of herpes simplex virus-1 infection suppress subsequent antiviral immunity, implicating impaired dendritic cell function. J Immunol. 2010;184:1867-75 pubmed publisher
Efimova O, Kelley T. Induction of granzyme B expression in T-cell receptor/CD28-stimulated human regulatory T cells is suppressed by inhibitors of the PI3K-mTOR pathway. BMC Immunol. 2009;10:59 pubmed publisher
Hoover R, Gullickson G, Kornbluth J. Impaired NK cytolytic activity and enhanced tumor growth in NK lytic-associated molecule-deficient mice. J Immunol. 2009;183:6913-21 pubmed publisher
Hamada H, Garcia Hernandez M, Reome J, Misra S, Strutt T, McKinstry K, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81 pubmed publisher
Andre P, Anfossi N. Clinical analysis of human natural killer cells. Methods Mol Biol. 2008;415:291-300 pubmed publisher
Yang T, Millar J, Groves T, Grinshtein N, Parsons R, Takenaka S, et al. The CD8+ T cell population elicited by recombinant adenovirus displays a novel partially exhausted phenotype associated with prolonged antigen presentation that nonetheless provides long-term immunity. J Immunol. 2006;176:200-10 pubmed
Matsuda J, Zhang Q, Ndonye R, Richardson S, Howell A, Gapin L. T-bet concomitantly controls migration, survival, and effector functions during the development of Valpha14i NKT cells. Blood. 2006;107:2797-805 pubmed
Shafer Weaver K, Sayers T, Strobl S, Derby E, Ulderich T, Baseler M, et al. The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity. J Transl Med. 2003;1:14 pubmed
Rininsland F, Helms T, Asaad R, Boehm B, Tary Lehmann M. Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity. J Immunol Methods. 2000;240:143-55 pubmed
Smyth M, Trapani J. Granzymes: exogenous proteinases that induce target cell apoptosis. Immunol Today. 1995;16:202-6 pubmed
Trinchieri G, Perussia B. Human natural killer cells: biologic and pathologic aspects. Lab Invest. 1984;50:489-513 pubmed
product information
Product Type :
Antibody
Product Name :
Granzyme B Monoclonal Antibody (GB11), PE, eBioscience
Catalog # :
12-8899-41
Quantity :
25 Tests
Price :
US 169.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 5 µL (0.06 µg)/test
Species :
Human
Clone :
GB11
Isotype :
IgG1
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 µL (0.06 µg)/test
Aliases :
AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA