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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
iNOS Monoclonal Antibody (CXNFT), PE, eBioscience
catalog :
12-5920-82
quantity :
100 ug
price :
US 426.00
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
CXNFT
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:50; loading ...; fig s3b
Yang C, Lei L, Collins J, Briones M, Ma L, Sturdevant G, et al. Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor. Nat Commun. 2021;12:5454 pubmed publisher
  • flow cytometry; mouse; loading ...
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5b
Papp S, Moderzynski K, Rauch J, Heine L, Kuehl S, Richardt U, et al. Liver Necrosis and Lethal Systemic Inflammation in a Murine Model of Rickettsia typhi Infection: Role of Neutrophils, Macrophages and NK Cells. PLoS Negl Trop Dis. 2016;10:e0004935 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 6b
Osterloh A, Papp S, Moderzynski K, Kuehl S, Richardt U, Fleischer B. Persisting Rickettsia typhi Causes Fatal Central Nervous System Inflammation. Infect Immun. 2016;84:1615-1632 pubmed publisher
Pizzurro G, Bridges K, Jiang X, Vidyarthi A, Miller Jensen K, Colegio O. Functionally and Metabolically Divergent Melanoma-Associated Macrophages Originate from Common Bone-Marrow Precursors. Cancers (Basel). 2023;15: pubmed publisher
Sheng W, Qin H, Wang T, Zhao J, Fang C, Zhang P, et al. Advanced phosphocreatine-grafted chitosan hydrogel promote wound healing by macrophage modulation. Front Bioeng Biotechnol. 2023;11:1199939 pubmed publisher
Prasad S, Singh A, Hu S, Sheng W, Chauhan P, Lokensgard J. Dysregulated brain regulatory T cells fail to control reactive gliosis following repeated antigen stimulation. iScience. 2023;26:106628 pubmed publisher
Liu X, Jiang Q, Shen S, Hou Y. Local and systemic inflammation triggers different outcomes of tumor growth related to infiltration of anti-tumor or pro-tumor macrophages. Chin Med J (Engl). 2022;135:1821-1828 pubmed publisher
Tai C, Xie Z, Li Y, Feng Y, Xie Y, Yang H, et al. Human skin dermis-derived fibroblasts are a kind of functional mesenchymal stromal cells: judgements from surface markers, biological characteristics, to therapeutic efficacy. Cell Biosci. 2022;12:105 pubmed publisher
Gao T, Huang F, Wang W, Xie Y, Wang B. Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages. Cell Mol Biol Lett. 2022;27:27 pubmed publisher
Bachy S, Wu Z, Gamradt P, Thierry K, Milani P, Chlasta J, et al. βig-h3-structured collagen alters macrophage phenotype and function in pancreatic cancer. iScience. 2022;25:103758 pubmed publisher
Liu X, Xu Y, Li Y, Pan Y, Zhao S, Hou Y. Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype. Int J Med Sci. 2021;18:3125-3139 pubmed publisher
Tan X, Banerjee P, Shi L, Xiao G, Rodriguez B, Grzeskowiak C, et al. p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Sci Adv. 2021;7: pubmed publisher
Ruiz Rosado J, Robledo Avila F, Cortado H, Rangel Moreno J, Justice S, Yang C, et al. Neutrophil-Macrophage Imbalance Drives the Development of Renal Scarring during Experimental Pyelonephritis. J Am Soc Nephrol. 2021;32:69-85 pubmed publisher
Zhang L, Wang W, Wang R, Zhang N, Shang H, Bi Y, et al. Reshaping the Immune Microenvironment by Oncolytic Herpes Simplex Virus in Murine Pancreatic Ductal Adenocarcinoma. Mol Ther. 2021;29:744-761 pubmed publisher
Hosomi K, Shibata N, Shimoyama A, Uto T, Nagatake T, Tojima Y, et al. Lymphoid Tissue-Resident Alcaligenes Establish an Intracellular Symbiotic Environment by Creating a Unique Energy Shift in Dendritic Cells. Front Microbiol. 2020;11:561005 pubmed publisher
Sun L, Girnary M, Wang L, Jiao Y, Zeng E, Mercer K, et al. IL-10 Dampens an IL-17-Mediated Periodontitis-Associated Inflammatory Network. J Immunol. 2020;204:2177-2191 pubmed publisher
Lee C, Seo I, Chae M, Park J, Choi E, Uhm H, et al. Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation. Oxid Med Cell Longev. 2020;2020:2946820 pubmed publisher
Brykczynska U, Geigges M, Wiedemann S, Dror E, Böni Schnetzler M, Hess C, et al. Distinct Transcriptional Responses across Tissue-Resident Macrophages to Short-Term and Long-Term Metabolic Challenge. Cell Rep. 2020;30:1627-1643.e7 pubmed publisher
Takada S, Sakakima H, Matsuyama T, Otsuka S, Nakanishi K, Norimatsu K, et al. Disruption of Midkine gene reduces traumatic brain injury through the modulation of neuroinflammation. J Neuroinflammation. 2020;17:40 pubmed publisher
Kim T, Park J, Kim D, Gautam A, Akauliya M, Kim J, et al. Anti-Bacterial Effect of CpG-DNA Involves Enhancement of the Complement Systems. Int J Mol Sci. 2019;20: pubmed publisher
Ribechini E, Eckert I, Beilhack A, du Plessis N, Walzl G, Schleicher U, et al. Heat-killed Mycobacterium tuberculosis prime-boost vaccination induces myeloid-derived suppressor cells with spleen dendritic cell-killing capability. JCI Insight. 2019;5: pubmed publisher
Kurelac I, Iommarini L, Vatrinet R, Amato L, De Luise M, Leone G, et al. Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses. Nat Commun. 2019;10:903 pubmed publisher
Chen D, Xie J, Fiskesund R, Dong W, Liang X, Lv J, et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun. 2018;9:873 pubmed publisher
Park H, Lee S, Kwon D, Heo S, Park S, Kim S, et al. Oral administration of taheebo (Tabebuia avellanedae Lorentz ex Griseb.) water extract prevents DSS-induced colitis in mice by up-regulating type II T helper immune responses. BMC Complement Altern Med. 2017;17:448 pubmed publisher
Giampazolias E, Zunino B, Dhayade S, Bock F, Cloix C, Cao K, et al. Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency. Nat Cell Biol. 2017;19:1116-1129 pubmed publisher
Nie H, Wang A, He Q, Yang Q, Liu L, Zhang G, et al. Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin-induced mouse model of asthma. Exp Ther Med. 2017;14:1284-1292 pubmed publisher
Buscher K, Ehinger E, Gupta P, Pramod A, Wolf D, Tweet G, et al. Natural variation of macrophage activation as disease-relevant phenotype predictive of inflammation and cancer survival. Nat Commun. 2017;8:16041 pubmed publisher
Jin W, Shi S, Li Z, Li M, Wood K, Gonzales R, et al. Depletion of microglia exacerbates postischemic inflammation and brain injury. J Cereb Blood Flow Metab. 2017;37:2224-2236 pubmed publisher
Hensel J, Khattar V, Ashton R, Lee C, Siegal G, Ponnazhagan S. Location of tumor affects local and distant immune cell type and number. Immun Inflamm Dis. 2017;5:85-94 pubmed publisher
Chauhan P, Hu S, Sheng W, Prasad S, Lokensgard J. Modulation of Microglial Cell Fcγ Receptor Expression Following Viral Brain Infection. Sci Rep. 2017;7:41889 pubmed publisher
Moderzynski K, Papp S, Rauch J, Heine L, Kuehl S, Richardt U, et al. CD4+ T Cells Are as Protective as CD8+ T Cells against Rickettsia typhi Infection by Activating Macrophage Bactericidal Activity. PLoS Negl Trop Dis. 2016;10:e0005089 pubmed publisher
Schleicher U, Paduch K, Debus A, Obermeyer S, König T, Kling J, et al. TNF-Mediated Restriction of Arginase 1 Expression in Myeloid Cells Triggers Type 2 NO Synthase Activity at the Site of Infection. Cell Rep. 2016;15:1062-1075 pubmed publisher
Ruiz Rosado J, Olguín J, Juarez Avelar I, Saavedra R, Terrazas L, Robledo Avila F, et al. MIF Promotes Classical Activation and Conversion of Inflammatory Ly6C(high) Monocytes into TipDCs during Murine Toxoplasmosis. Mediators Inflamm. 2016;2016:9101762 pubmed publisher
Smolders S, Smolders S, Swinnen N, Gärtner A, Rigo J, Legendre P, et al. Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo. Front Cell Neurosci. 2015;9:301 pubmed publisher
Lee S, Park H, Jeon S, Lee C, Seong R, Park S, et al. Ubiquitous Over-Expression of Chromatin Remodeling Factor SRG3 Ameliorates the T Cell-Mediated Exacerbation of EAE by Modulating the Phenotypes of both Dendritic Cells and Macrophages. PLoS ONE. 2015;10:e0132329 pubmed publisher
Mairhofer D, Ortner D, Tripp C, Schaffenrath S, Fleming V, Heger L, et al. Impaired gp100-Specific CD8(+) T-Cell Responses in the Presence of Myeloid-Derived Suppressor Cells in a Spontaneous Mouse Melanoma Model. J Invest Dermatol. 2015;135:2785-2793 pubmed publisher
Shen L, Sundstedt A, Ciesielski M, Miles K, Celander M, Adelaiye R, et al. Tasquinimod modulates suppressive myeloid cells and enhances cancer immunotherapies in murine models. Cancer Immunol Res. 2015;3:136-48 pubmed publisher
Nakanishi Y, Sato T, Ohteki T. Commensal Gram-positive bacteria initiates colitis by inducing monocyte/macrophage mobilization. Mucosal Immunol. 2015;8:152-60 pubmed publisher
product information
Product Type :
Antibody
Product Name :
iNOS Monoclonal Antibody (CXNFT), PE, eBioscience
Catalog # :
12-5920-82
Quantity :
100 ug
Price :
US 426.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.06 ug/test
Species :
Mouse
Clone :
CXNFT
Isotype :
IgG2a, kappa
Storage :
4 C, store in dark, DO NOT FREEZE!
Description :
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.06 ug/test
Aliases :
Hepatocyte NOS; hepatocytes; HEP-NOS; inducible nitric oxide synthase; inducible NO synthase; Inducible NOS; iNos; i-NOS; Inosl; MAC-NOS; macrophage NOS; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS; NOS type II; NOS, type II; Nos2; Nos-2; Nos2a; NOS-II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA