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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
FOXP3 Monoclonal Antibody (NRRF-30), PE, eBioscience
catalog :
12-4771-82
quantity :
100 µg
price :
US 472.00
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
NRRF-30
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 62
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 5a, 5c
Lu L, Li T, Feng X, Liu Z, Liu Y, Chao T, et al. Excessive immunosuppression by regulatory T cells antagonizes T cell response to schistosome infection in PD-1-deficient mice. PLoS Pathog. 2022;18:e1010596 pubmed publisher
  • flow cytometry; mouse; fig 1
Patel M, Kim J, Theodros D, Tam A, Velarde E, Kochel C, et al. Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma. J Immunother Cancer. 2016;4:28 pubmed publisher
Zhang W, Li I, Reticker Flynn N, Good Z, Chang S, Samusik N, et al. Identification of cell types in multiplexed in situ images by combining protein expression and spatial information using CELESTA. Nat Methods. 2022;19:759-769 pubmed publisher
Tan Y, Ding Y, Zheng X, Dai G, Zhang S, Yang X, et al. Ding's herbal enema treats dextran sulfate sodium-induced colitis in mice by regulating the gut microbiota and maintaining the Treg/Th17 cell balance. Exp Ther Med. 2021;22:1368 pubmed publisher
Porsche C, Delproposto J, Geletka L, O ROURKE R, Lumeng C. Obesity results in adipose tissue T cell exhaustion. JCI Insight. 2021;6: pubmed publisher
De Martino M, Daviaud C, Diamond J, Kraynak J, Alard A, Formenti S, et al. Activin A Promotes Regulatory T-cell-Mediated Immunosuppression in Irradiated Breast Cancer. Cancer Immunol Res. 2021;9:89-102 pubmed publisher
Liu Y, Yin Z, Lu P, Ma Y, Luo B, Xiang L, et al. Lung Carcinoma Cells Secrete Exosomal MALAT1 to Inhibit Dendritic Cell Phagocytosis, Inflammatory Response, Costimulatory Molecule Expression and Promote Dendritic Cell Autophagy via AKT/mTOR Pathway. Onco Targets Ther. 2020;13:10693-10705 pubmed publisher
Hanson A, Elpek K, Duong E, Shallberg L, Fan M, Johnson C, et al. ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models. PLoS ONE. 2020;15:e0239595 pubmed publisher
Yi X, Zhou H, Chao Y, Xiong S, Zhong J, Chai Z, et al. Bacteria-triggered tumor-specific thrombosis to enable potent photothermal immunotherapy of cancer. Sci Adv. 2020;6:eaba3546 pubmed publisher
Yang R, Huang H, Cui S, Zhou Y, Zhang T, Zhou Y. IFN-γ promoted exosomes from mesenchymal stem cells to attenuate colitis via miR-125a and miR-125b. Cell Death Dis. 2020;11:603 pubmed publisher
Ko J, Kim H, Jeong H, Lee H, Oh J. Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis. Cell Rep. 2020;30:3806-3820.e6 pubmed publisher
Porsche C, Delproposto J, Patrick E, Zamarron B, Lumeng C. Adipose tissue dendritic cell signals are required to maintain T cell homeostasis and obesity-induced expansion. Mol Cell Endocrinol. 2020;505:110740 pubmed publisher
Yue W, Chen L, Yu L, Zhou B, Yin H, Ren W, et al. Checkpoint blockade and nanosonosensitizer-augmented noninvasive sonodynamic therapy combination reduces tumour growth and metastases in mice. Nat Commun. 2019;10:2025 pubmed publisher
Lee H, Kim K, Ryu J, Jeong H, Lee S, Kim M. Bilateral Effect of the Unilateral Corneal Nerve Cut on Both Ocular Surface and Lacrimal Gland. Invest Ophthalmol Vis Sci. 2019;60:430-441 pubmed publisher
Moreno A, Porchia B, Pagni R, Souza P, Pegoraro R, Rodrigues K, et al. The Combined Use of Melatonin and an Indoleamine 2,3-Dioxygenase-1 Inhibitor Enhances Vaccine-Induced Protective Cellular Immunity to HPV16-Associated Tumors. Front Immunol. 2018;9:1914 pubmed publisher
He Y, Yuan X, Li Y, Zhong C, Liu Y, Qian H, et al. Loss of Gαq impairs regulatory B-cell function. Arthritis Res Ther. 2018;20:186 pubmed publisher
Yoshida M, Taguchi A, Kawana K, Ogishima J, Adachi K, Kawata A, et al. Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity. Int J Oncol. 2018;53:1580-1590 pubmed publisher
Xu G, Wang T, Li Y, Huang Z, Wang X, Zheng J, et al. A highly potent and selective inhibitor Roxyl-WL targeting IDO1 promotes immune response against melanoma. J Enzyme Inhib Med Chem. 2018;33:1089-1094 pubmed publisher
Wang T, Wang D, Yu H, Feng B, Zhou F, Zhang H, et al. A cancer vaccine-mediated postoperative immunotherapy for recurrent and metastatic tumors. Nat Commun. 2018;9:1532 pubmed publisher
Turaj A, Hussain K, Cox K, Rose Zerilli M, Testa J, Dahal L, et al. Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment. Cancer Cell. 2017;32:777-791.e6 pubmed publisher
Hu X, Li X, Hu C, Qin L, He R, Luo L, et al. Respiratory Syncytial Virus Exacerbates OVA-mediated asthma in mice through C5a-C5aR regulating CD4+T cells Immune Responses. Sci Rep. 2017;7:15207 pubmed publisher
Porchia B, Moreno A, Ramos R, Diniz M, de Andrade L, Rosa D, et al. Herpes Simplex Virus Glycoprotein D Targets a Specific Dendritic Cell Subset and Improves the Performance of Vaccines to Human Papillomavirus-Associated Tumors. Mol Cancer Ther. 2017;16:1922-1933 pubmed publisher
Chen Q, Xu L, Liang C, Wang C, Peng R, Liu Z. Photothermal therapy with immune-adjuvant nanoparticles together with checkpoint blockade for effective cancer immunotherapy. Nat Commun. 2016;7:13193 pubmed publisher
Marinho F, Alves C, de Souza S, da Silva C, Cassali G, Oliveira S, et al. Schistosoma mansoni Tegument (Smteg) Induces IL-10 and Modulates Experimental Airway Inflammation. PLoS ONE. 2016;11:e0160118 pubmed publisher
Andersson S, Eneljung T, Tengvall S, Jirholt P, Stern A, Henningsson L, et al. Collagen epitope expression on B cells is sufficient to confer tolerance to collagen-induced arthritis. Arthritis Res Ther. 2016;18:140 pubmed publisher
Tengvall S, Eneljung T, Jirholt P, Turesson O, Wing K, Holmdahl R, et al. Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis. PLoS ONE. 2016;11:e0154630 pubmed publisher
Li Y, Fang M, Zhang J, Wang J, Song Y, Shi J, et al. Hydrogel dual delivered celecoxib and anti-PD-1 synergistically improve antitumor immunity. Oncoimmunology. 2016;5:e1074374 pubmed
Kim W, Kim J, Cha S, Han S, Kim H, Kwon K, et al. Virulence-Dependent Alterations in the Kinetics of Immune Cells during Pulmonary Infection by Mycobacterium tuberculosis. PLoS ONE. 2015;10:e0145234 pubmed publisher
Pallangyo C, Ziegler P, Greten F. IKKβ acts as a tumor suppressor in cancer-associated fibroblasts during intestinal tumorigenesis. J Exp Med. 2015;212:2253-66 pubmed publisher
Lee S, Park H, Jeon S, Lee C, Seong R, Park S, et al. Ubiquitous Over-Expression of Chromatin Remodeling Factor SRG3 Ameliorates the T Cell-Mediated Exacerbation of EAE by Modulating the Phenotypes of both Dendritic Cells and Macrophages. PLoS ONE. 2015;10:e0132329 pubmed publisher
Devaud C, Yong C, John L, Westwood J, Duong C, House C, et al. Foxp3 expression in macrophages associated with RENCA tumors in mice. PLoS ONE. 2014;9:e108670 pubmed publisher
Maneva Radicheva L, Amatya C, Parker C, Ellefson J, Radichev I, Raghavan A, et al. Autoimmune diabetes is suppressed by treatment with recombinant human tissue Kallikrein-1. PLoS ONE. 2014;9:e107213 pubmed publisher
Chan M, Chang C, Tung C, Sun J, Suen J, Wu S. Low-dose 5-aza-2'-deoxycytidine pretreatment inhibits experimental autoimmune encephalomyelitis by induction of regulatory T cells. Mol Med. 2014;20:248-56 pubmed publisher
Eneljung T, Tengvall S, Jirholt P, Henningsson L, Holmdahl R, Gustafsson K, et al. Antigen-specific gene therapy after immunisation reduces the severity of collagen-induced arthritis. Clin Dev Immunol. 2013;2013:345092 pubmed publisher
Wong L, HATFIELD J, Brown M. Ikaros sets the potential for Th17 lineage gene expression through effects on chromatin state in early T cell development. J Biol Chem. 2013;288:35170-9 pubmed publisher
Yolcu E, Kaminitz A, Mizrahi K, Ash S, Yaniv I, Stein J, et al. Immunomodulation with donor regulatory T cells armed with Fas-ligand alleviates graft-versus-host disease. Exp Hematol. 2013;41:903-11 pubmed publisher
Kaminitz A, Yolcu E, Mizrahi K, Shirwan H, Askenasy N. Killer Treg cells ameliorate inflammatory insulitis in non-obese diabetic mice through local and systemic immunomodulation. Int Immunol. 2013;25:485-94 pubmed publisher
Amoah S, Holbrook B, Yammani R, Alexander Miller M. High viral burden restricts short-lived effector cell number at late times postinfection through increased natural regulatory T cell expansion. J Immunol. 2013;190:5020-9 pubmed publisher
Kim M, Kim T. R-phycoerythrin-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells. Cytometry A. 2013;83:452-60 pubmed publisher
Tasso R, Ilengo C, Quarto R, Cancedda R, Caspi R, Pennesi G. Mesenchymal stem cells induce functionally active T-regulatory lymphocytes in a paracrine fashion and ameliorate experimental autoimmune uveitis. Invest Ophthalmol Vis Sci. 2012;53:786-93 pubmed publisher
Yarkoni S, Prigozhina T, Slavin S, Askenasy N. IL-2-targeted therapy ameliorates the severity of graft-versus-host disease: ex vivo selective depletion of host-reactive T cells and in vivo therapy. Biol Blood Marrow Transplant. 2012;18:523-35 pubmed publisher
Younes S, Punkosdy G, Caucheteux S, Chen T, Grossman Z, Paul W. Memory phenotype CD4 T cells undergoing rapid, nonburst-like, cytokine-driven proliferation can be distinguished from antigen-experienced memory cells. PLoS Biol. 2011;9:e1001171 pubmed publisher
Rowswell Turner R, Harden J, Nair R, Gu T, Kilinc M, Egilmez N. Chronic chemoimmunotherapy achieves cure of spontaneous murine mammary tumors via persistent blockade of posttherapy counter-regulation. J Immunol. 2011;187:4109-18 pubmed publisher
Whitehead G, Wilson R, Nakano K, Burch L, Nakano H, Cook D. IL-35 production by inducible costimulator (ICOS)-positive regulatory T cells reverses established IL-17-dependent allergic airways disease. J Allergy Clin Immunol. 2012;129:207-15.e1-5 pubmed publisher
Kaminitz A, Yolcu E, Askenasy E, Stein J, Yaniv I, Shirwan H, et al. Effector and naturally occurring regulatory T cells display no abnormalities in activation induced cell death in NOD mice. PLoS ONE. 2011;6:e21630 pubmed publisher
Gupta G, Majumdar S, Adhikari A, Bhattacharya P, Mukherjee A, Majumdar S, et al. Treatment with IP-10 induces host-protective immune response by regulating the T regulatory cell functioning in Leishmania donovani-infected mice. Med Microbiol Immunol. 2011;200:241-53 pubmed publisher
Kaminitz A, Yolcu E, Stein J, Yaniv I, Shirwan H, Askenasy N. Killer Treg restore immune homeostasis and suppress autoimmune diabetes in prediabetic NOD mice. J Autoimmun. 2011;37:39-47 pubmed publisher
Fujita M, Kohanbash G, Fellows Mayle W, Hamilton R, Komohara Y, Decker S, et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res. 2011;71:2664-74 pubmed publisher
Libregts S, van Olffen R, Van Der Sluijs K, van Lier R, Nolte M. Function of CD27 in helper T cell differentiation. Immunol Lett. 2011;136:177-86 pubmed publisher
Kaminitz A, Askenasy E, Yaniv I, Stein J, Askenasy N. Apoptosis of purified CD4+ T cell subsets is dominated by cytokine deprivation and absence of other cells in new onset diabetic NOD mice. PLoS ONE. 2010;5:e15684 pubmed publisher
Clutter M, Heffner G, Krutzik P, Sachen K, Nolan G. Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry. Cytometry A. 2010;77:1020-31 pubmed publisher
Batsalova T, Dzhambazov B, Klaczkowska D, Holmdahl R. Mice producing less reactive oxygen species are relatively resistant to collagen glycopeptide vaccination against arthritis. J Immunol. 2010;185:2701-9 pubmed publisher
Kaminitz A, Mizrahi K, Yaniv I, Stein J, Askenasy N. Immunosuppressive therapy exacerbates autoimmunity in NOD mice and diminishes the protective activity of regulatory T cells. J Autoimmun. 2010;35:145-52 pubmed publisher
Fujita M, Scheurer M, Decker S, McDonald H, Kohanbash G, Kastenhuber E, et al. Role of type 1 IFNs in antiglioma immunosurveillance--using mouse studies to guide examination of novel prognostic markers in humans. Clin Cancer Res. 2010;16:3409-19 pubmed publisher
Amu S, Saunders S, Kronenberg M, Mangan N, Atzberger A, Fallon P. Regulatory B cells prevent and reverse allergic airway inflammation via FoxP3-positive T regulatory cells in a murine model. J Allergy Clin Immunol. 2010;125:1114-1124.e8 pubmed publisher
Ueda R, Fujita M, Zhu X, Sasaki K, Kastenhuber E, Kohanbash G, et al. Systemic inhibition of transforming growth factor-beta in glioma-bearing mice improves the therapeutic efficacy of glioma-associated antigen peptide vaccines. Clin Cancer Res. 2009;15:6551-9 pubmed publisher
Sagiv Y, Kaminitz A, Lorberboum Galski H, Askenasy N, Yarkoni S. A fusion protein composed of IL-2 and caspase-3 ameliorates the outcome of experimental inflammatory colitis. Ann N Y Acad Sci. 2009;1173:791-7 pubmed publisher
O Gorman W, Dooms H, Thorne S, Kuswanto W, Simonds E, Krutzik P, et al. The initial phase of an immune response functions to activate regulatory T cells. J Immunol. 2009;183:332-9 pubmed publisher
van Olffen R, Koning N, van Gisbergen K, Wensveen F, Hoek R, Boon L, et al. GITR triggering induces expansion of both effector and regulatory CD4+ T cells in vivo. J Immunol. 2009;182:7490-500 pubmed publisher
Atarashi K, Mori T, Yoshiki R, Kabashima K, Kuma H, Tokura Y. Skin application of ketoprofen systemically suppresses contact hypersensitivity by inducing CD4(+) CD25(+) regulatory T cells. J Dermatol Sci. 2009;53:216-21 pubmed publisher
de Zoeten E, Lee I, Wang L, Chen C, Ge G, Wells A, et al. Foxp3 processing by proprotein convertases and control of regulatory T cell function. J Biol Chem. 2009;284:5709-16 pubmed publisher
Toubaji A, Hill S, Terabe M, Qian J, Floyd T, Simpson R, et al. The combination of GM-CSF and IL-2 as local adjuvant shows synergy in enhancing peptide vaccines and provides long term tumor protection. Vaccine. 2007;25:5882-91 pubmed
product information
Product Type :
Antibody
Product Name :
FOXP3 Monoclonal Antibody (NRRF-30), PE, eBioscience
Catalog # :
12-4771-82
Quantity :
100 µg
Price :
US 472.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.25 µg/test
Species :
Mouse
Clone :
NRRF-30
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.25 µg/test
Aliases :
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA