CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PE, eBioscience™ | Invitrogen 12-0951-83 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PE, eBioscience™

catalog :

12-0951-83

quantity :

200 µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

15A7

reactivity :

mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 38

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	Kim Y, Lim H, Jung H, Wetsel R, Chung Y. Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS ONE. 2015;10:e0120294 pubmed publisher
	Anders P, Montgomery N, Montgomery S, Bhatt A, Dittmer D, Damania B. Human herpesvirus-encoded kinase induces B cell lymphomas in vivo. J Clin Invest. 2018;128:2519-2534 pubmed publisher
	Liu J, Huang X, Hao S, Wang Y, Liu M, Xu J, et al. Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus. Nat Commun. 2018;9:1136 pubmed publisher
	Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
	YEKU O, Purdon T, Koneru M, Spriggs D, Brentjens R. Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment. Sci Rep. 2017;7:10541 pubmed publisher
	Degn S, van der Poel C, Firl D, Ayoglu B, Al Qureshah F, Bajic G, et al. Clonal Evolution of Autoreactive Germinal Centers. Cell. 2017;170:913-926.e19 pubmed publisher
	Tang H, Wang H, Lin Q, Fan F, Zhang F, Peng X, et al. Loss of IP3 Receptor-Mediated Ca2+ Release in Mouse B Cells Results in Abnormal B Cell Development and Function. J Immunol. 2017;199:570-580 pubmed publisher
	Kamei R, Tanaka H, Kawano T, Morii C, Tanaka S, Nishihara H, et al. Regulation of endothelial Fas expression as a mechanism of promotion of vascular integrity by mural cells in tumors. Cancer Sci. 2017;108:1080-1088 pubmed publisher
	Nguyen T, Kläsener K, Zürn C, Castillo P, Brust Mascher I, Imai D, et al. The IgM receptor Fc?R limits tonic BCR signaling by regulating expression of the IgM BCR. Nat Immunol. 2017;18:321-333 pubmed publisher
	Kleiman E, Jia H, Loguercio S, Su A, Feeney A. YY1 plays an essential role at all stages of B-cell differentiation. Proc Natl Acad Sci U S A. 2016;113:E3911-20 pubmed publisher
	Ortega Molina A, Boss I, Canela A, Pan H, Jiang Y, Zhao C, et al. The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development. Nat Med. 2015;21:1199-208 pubmed publisher
	Zhao H, Bauzon F, Bi E, Yu J, Fu H, Lu Z, et al. Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age. J Biol Chem. 2015;290:5797-809 pubmed publisher
	Song L, Yang M, Knoff J, Wu T, Hung C. Cancer immunotherapy employing an innovative strategy to enhance CD4+ T cell help in the tumor microenvironment. PLoS ONE. 2014;9:e115711 pubmed publisher
	Wolf A, Strauman M, Mozdzanowska K, Whittle J, Williams K, Sharpe A, et al. Coinfection with Streptococcus pneumoniae modulates the B cell response to influenza virus. J Virol. 2014;88:11995-2005 pubmed publisher
	Ding Y, Li J, Yang P, Luo B, Wu Q, Zajac A, et al. Interleukin-21 promotes germinal center reaction by skewing the follicular regulatory T cell to follicular helper T cell balance in autoimmune BXD2 mice. Arthritis Rheumatol. 2014;66:2601-12 pubmed publisher
	Li B, Sun M, Gao F, Liu W, Yang Y, Liu H, et al. Up-regulated expression of miR-23a/b targeted the pro-apoptotic Fas in radiation-induced thymic lymphoma. Cell Physiol Biochem. 2013;32:1729-40 pubmed publisher
	Ding Y, Li J, Wu Q, Yang P, Luo B, Xie S, et al. IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells. J Immunol. 2013;191:1614-24 pubmed publisher
	Chu Y, Soberon V, Glockner L, Beyaert R, Massoumi R, Van Loo G, et al. A20 and CYLD do not share significant overlapping functions during B cell development and activation. J Immunol. 2012;189:4437-43 pubmed publisher
	Nakagawa Y, Shimizu M, Norose Y, Takahashi M, Takahashi H. Induction of rapid apoptosis for class I MHC molecule-restricted CD8(+) HIV-1 gp160-specific murine activated CTLs by free antigenic peptide in vivo. Int Immunol. 2013;25:11-24 pubmed publisher
	Garchow B, Bartulos Encinas O, Leung Y, Tsao P, Eisenberg R, Caricchio R, et al. Silencing of microRNA-21 in vivo ameliorates autoimmune splenomegaly in lupus mice. EMBO Mol Med. 2011;3:605-15 pubmed publisher
	Green B, Belcheva A, Nepal R, Boulianne B, Martin A. The mismatch repair pathway functions normally at a non-AID target in germinal center B cells. Blood. 2011;118:3013-8 pubmed publisher
	Tabbekh M, Franciszkiewicz K, Haouas H, Lecluse Y, Benihoud K, Raman C, et al. Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice. J Immunol. 2011;187:102-9 pubmed publisher
	Huang F, Barnes P, Feng Y, Donis R, Chroneos Z, Idell S, et al. GM-CSF in the lung protects against lethal influenza infection. Am J Respir Crit Care Med. 2011;184:259-68 pubmed publisher
	Chu Y, Vahl J, Kumar D, Heger K, Bertossi A, Wójtowicz E, et al. B cells lacking the tumor suppressor TNFAIP3/A20 display impaired differentiation and hyperactivation and cause inflammation and autoimmunity in aged mice. Blood. 2011;117:2227-36 pubmed publisher
	Pearl Yafe M, Mizrahi K, Stein J, Yolcu E, Kaplan O, Shirwan H, et al. Tumor necrosis factor receptors support murine hematopoietic progenitor function in the early stages of engraftment. Stem Cells. 2010;28:1270-80 pubmed publisher
	Bolduc A, Long E, Stapler D, Cascalho M, Tsubata T, Koni P, et al. Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J Immunol. 2010;185:220-30 pubmed publisher
	Zaheen A, Boulianne B, Parsa J, Ramachandran S, Gommerman J, Martin A. AID constrains germinal center size by rendering B cells susceptible to apoptosis. Blood. 2009;114:547-54 pubmed publisher
	Lee S, Salek Ardakani S, Mittler R, Croft M. Hypercostimulation through 4-1BB distorts homeostasis of immune cells. J Immunol. 2009;182:6753-62 pubmed publisher
	Mise Omata S, Kuroda E, Sugiura T, Yamashita U, Obata Y, Doi T. The NF-kappaB RelA subunit confers resistance to Leishmania major by inducing nitric oxide synthase 2 and Fas expression but not Th1 differentiation. J Immunol. 2009;182:4910-6 pubmed publisher
	Wang Y, Diamond B. B cell receptor revision diminishes the autoreactive B cell response after antigen activation in mice. J Clin Invest. 2008;118:2896-907 pubmed publisher
	Nepal R, Zaheen A, Basit W, Li L, Berger S, Martin A. AID and RAG1 do not contribute to lymphomagenesis in Emu c-myc transgenic mice. Oncogene. 2008;27:4752-6 pubmed publisher
	Treese C, Mittag A, Lange F, Tarnok A, Loesche A, Emmrich F, et al. Characterization of fibroblasts responsible for cartilage destruction in arthritis. Cytometry A. 2008;73:351-60 pubmed publisher
	Pearl Yafe M, Yolcu E, Stein J, Kaplan O, Shirwan H, Yaniv I, et al. Expression of Fas and Fas-ligand in donor hematopoietic stem and progenitor cells is dissociated from the sensitivity to apoptosis. Exp Hematol. 2007;35:1601-12 pubmed
	Pearl Yafe M, Stein J, Yolcu E, Farkas D, Shirwan H, Yaniv I, et al. Fas transduces dual apoptotic and trophic signals in hematopoietic progenitors. Stem Cells. 2007;25:3194-203 pubmed
	Li M, Wang Y, Guo R, Bai Y, Yu Z. Glucocorticoids impair microglia ability to induce T cell proliferation and Th1 polarization. Immunol Lett. 2007;109:129-37 pubmed
	Peshes Yaloz N, Rosen D, Sondel P, Krammer P, Berke G. Up-regulation of Fas (CD95) expression in tumour cells in vivo. Immunology. 2007;120:502-11 pubmed
	Aloman C, Gehring S, Wintermeyer P, Kuzushita N, Wands J. Chronic ethanol consumption impairs cellular immune responses against HCV NS5 protein due to dendritic cell dysfunction. Gastroenterology. 2007;132:698-708 pubmed
	Podojil J, Kohm A, Miller S. CD4+ T cell expressed CD80 regulates central nervous system effector function and survival during experimental autoimmune encephalomyelitis. J Immunol. 2006;177:2948-58 pubmed

product information

Product Type :

Antibody

Product Name :

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PE, eBioscience™

Catalog # :

12-0951-83

Quantity :

200 µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Mouse

Applications :

Flow Cytometry: 1 µg/test

Species :

Mouse

Clone :

15A7

Isotype :

IgG1, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test

Aliases :

AI196731; ALPS1A; APO1; APO-1; Apo1 antigen; Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; APT1; CD95; CD95 antigen; FAS; Fas (TNF receptor superfamily member 6); Fas (TNF receptor superfamily member); Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas antigen (ATP1); Fas cell surface death receptor; Fas receptor; FAS1; FASLG receptor; FASTM; lpr; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; TNFR6; Tnfrsf6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 26; Tumor necrosis factor receptor superfamily, member 6

more info or order :

Invitrogen product webpage