product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD86 (B7-2) Monoclonal Antibody (IT2.2), PE, eBioscience
catalog :
12-0869-41
quantity :
25 Tests
price :
US 96.00
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
IT2.2
reactivity :
human
application :
flow cytometry
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s3a
Ahmed R, Omidian Z, Giwa A, Cornwell B, Majety N, Bell D, et al. A Public BCR Present in a Unique Dual-Receptor-Expressing Lymphocyte from Type 1 Diabetes Patients Encodes a Potent T Cell Autoantigen. Cell. 2019;177:1583-1599.e16 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Gentili M, Lahaye X, Nadalin F, Nader G, Puig Lombardi E, Hervé S, et al. The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus. Cell Rep. 2019;26:2377-2393.e13 pubmed publisher
  • flow cytometry; human; loading ...; fig 3e
Ha D, Tanaka A, Kibayashi T, Tanemura A, Sugiyama D, Wing J, et al. Differential control of human Treg and effector T cells in tumor immunity by Fc-engineered anti-CTLA-4 antibody. Proc Natl Acad Sci U S A. 2019;116:609-618 pubmed publisher
  • flow cytometry; human; loading ...; fig s4a
Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg M, et al. Intrinsic antiproliferative activity of the innate sensor STING in T lymphocytes. J Exp Med. 2017;214:1769-1785 pubmed publisher
  • flow cytometry; human
Fricke F, Beaudouin J, Eils R, Heilemann M. One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy. Sci Rep. 2015;5:14072 pubmed publisher
  • flow cytometry; human
O Regan N, Steinfelder S, Venugopal G, Rao G, Lucius R, Srikantam A, et al. Brugia malayi microfilariae induce a regulatory monocyte/macrophage phenotype that suppresses innate and adaptive immune responses. PLoS Negl Trop Dis. 2014;8:e3206 pubmed publisher
  • flow cytometry; human
Longman R, Diehl G, Victorio D, Huh J, Galan C, Miraldi E, et al. CX?CR1? mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22. J Exp Med. 2014;211:1571-83 pubmed publisher
Shen H, Xu J, Zhang W, Chen Q, Zhu Z, Mao Y. Exosomal circRHCG promotes breast cancer metastasis via facilitating M2 polarization through TFEB ubiquitination and degradation. NPJ Precis Oncol. 2024;8:22 pubmed publisher
Liu H, Tang L, Gong S, Xiao T, Yang H, Gu W, et al. USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24. Cell Death Dis. 2023;14:852 pubmed publisher
Lang L, Zheng D, Jiang Q, Meng T, Ma X, Yang Y. Uncoupling protein 2 modulates polarization and metabolism of human primary macrophages via glycolysis and the NF‑κB pathway. Exp Ther Med. 2023;26:583 pubmed publisher
Zhang J, Yuan Z, Li X, Wang F, Wei X, Kang Y, et al. Activation of the JNK/COX-2/HIF-1α axis promotes M1 macrophage via glycolytic shift in HIV-1 infection. Life Sci Alliance. 2023;6: pubmed publisher
Zhao M, Chen L, Yang J, Zhang Z, Wang H, Shao Z, et al. Interleukin 6 exacerbates the progression of warm autoimmune hemolytic anemia by influencing the activity and function of B cells. Sci Rep. 2023;13:13231 pubmed publisher
Elsayed R, Elashiry M, Tran C, Yang T, Carroll A, Liu Y, et al. Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation. Int J Mol Sci. 2023;24: pubmed publisher
Wang W, Li X, Qin X, Miao Y, Zhang Y, Li S, et al. Germline Neurofibromin 1 mutation enhances the anti-tumour immune response and decreases juvenile myelomonocytic leukaemia tumourigenicity. Br J Haematol. 2023;202:328-343 pubmed publisher
Thaweerattanasinp T, Wanitchang A, Saenboonrueng J, Srisutthisamphan K, Wanasen N, Sungsuwan S, et al. SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages. Peerj. 2023;11:e14918 pubmed publisher
Cho S, Jeong S, Jeon Y, Yang S, Hwang J, Yoo B, et al. WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy. Int J Mol Sci. 2023;24: pubmed publisher
Fang J, Ou Q, Wu B, Li S, Wu M, Qiu J, et al. TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection. Cells. 2022;11: pubmed publisher
Fosdick M, Loftus S, Phillips I, Zacharias Z, Houtman J. Glycerol monolaurate inhibition of human B cell activation. Sci Rep. 2022;12:13506 pubmed publisher
Jiang H, Zhou L, Shen N, Ning X, Wu D, Jiang K, et al. M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway. Cell Death Dis. 2022;13:183 pubmed publisher
El Houseini M, Arafat M, El Husseiny A, Kasem I, Kamel M, El Habashy A, et al. Biological and molecular studies on specific immune cells treated with checkpoint inhibitors for the thera-personal approach of breast cancer patients (ex-vivo study). Oncol Res. 2021;29:319-330 pubmed publisher
Shukla S, Ying W, Gray F, Yao Y, Simes M, Zhao Q, et al. Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain. Nat Chem Biol. 2021;17:784-793 pubmed publisher
Li Q, Li X, Quan H, Wang Y, Qu G, Shen Z, et al. IL-10-/- Enhances DCs Immunity Against Chlamydia psittaci Infection via OX40L/NLRP3 and IDO/Treg Pathways. Front Immunol. 2021;12:645653 pubmed publisher
Szabo P, Dogra P, Gray J, Wells S, Connors T, Weisberg S, et al. Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19. Immunity. 2021;54:797-814.e6 pubmed publisher
Li G, Cui L, Wang G. miR-155-5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis. Exp Ther Med. 2021;21:68 pubmed publisher
Xie J, Yuan S, Peng L, Li H, Niu L, Xu H, et al. Antitumor immunity targeting fibroblast activation protein-α in a mouse Lewis lung carcinoma model. Oncol Lett. 2020;20:868-876 pubmed publisher
Sumransub N, Jirapongwattana N, Jamjuntra P, Thongchot S, Chieochansin T, Yenchitsomanus P, et al. Breast cancer stem cell RNA-pulsed dendritic cells enhance tumor cell killing by effector T cells. Oncol Lett. 2020;19:2422-2430 pubmed publisher
Ko J, Kim H, Jeong H, Lee H, Oh J. Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis. Cell Rep. 2020;30:3806-3820.e6 pubmed publisher
Perot B, Garcia Paredes V, Luka M, Ménager M. Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4+ T Lymphocytes. Front Cell Infect Microbiol. 2020;10:70 pubmed publisher
Liu W, Zhu M, Wang H, Wang W, Lu Y. Diffuse large B cell lymphoma-derived extracellular vesicles educate macrophages to promote tumours progression by increasing PGC-1β. Scand J Immunol. 2020;91:e12841 pubmed publisher
Lopes D, Oliveira S, Page B, Carvalho L, Carvalho E, Cardoso L. Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis. Front Immunol. 2018;9:3122 pubmed publisher
Tomic S, Ilic N, Kokol V, Gruden Movsesijan A, Mihajlović D, Bekić M, et al. Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells. Int J Nanomedicine. 2018;13:6941-6960 pubmed publisher
Chiu Y, Shu K, Yang F, Chou T, Chen P, Lay F, et al. A comprehensive characterization of aggravated aging-related changes in T lymphocytes and monocytes in end-stage renal disease: the iESRD study. Immun Ageing. 2018;15:27 pubmed publisher
Zhang F, Zhu Y, Fan G, Hu S. Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70. Oncol Lett. 2018;16:5034-5040 pubmed publisher
Xiu W, Ma J, Lei T, Zhang M. AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells. Oncol Lett. 2018;16:2851-2856 pubmed publisher
Stein J, Xu Q, Jackson K, Romm E, Wuest S, Kosa P, et al. Intrathecal B Cells in MS Have Significantly Greater Lymphangiogenic Potential Compared to B Cells Derived From Non-MS Subjects. Front Neurol. 2018;9:554 pubmed publisher
Li M, Zhao L, Zhang C, Wang X, Hong W, Sun J, et al. Dengue immune sera enhance Zika virus infection in human peripheral blood monocytes through Fc gamma receptors. PLoS ONE. 2018;13:e0200478 pubmed publisher
Guo M, Xiao J, Sheng X, Zhang X, Tie Y, Wang L, et al. Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype. Front Pharmacol. 2018;9:464 pubmed publisher
Johnson J, Lucas S, Amon L, Skelton S, Nazitto R, Carbonetti S, et al. Reshaping of the Dendritic Cell Chromatin Landscape and Interferon Pathways during HIV Infection. Cell Host Microbe. 2018;23:366-381.e9 pubmed publisher
Hammer A, Waschbisch A, Knippertz I, Zinser E, Berg J, Jörg S, et al. Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 Signaling for Effects of Fumaric Acid Esters on Dendritic Cells. Front Immunol. 2017;8:1922 pubmed publisher
Wu X, Xu L, Shen Y, Yu N, Zhang Y, Guo T. MALP-2, an agonist of TLR6, promotes the immune status without affecting the differentiation capacity of umbilical cord mesenchymal stem cells. Exp Ther Med. 2017;14:5540-5546 pubmed publisher
García González P, Schinnerling K, Sepúlveda Gutiérrez A, Maggi J, Mehdi A, Nel H, et al. Dexamethasone and Monophosphoryl Lipid A Induce a Distinctive Profile on Monocyte-Derived Dendritic Cells through Transcriptional Modulation of Genes Associated With Essential Processes of the Immune Response. Front Immunol. 2017;8:1350 pubmed publisher
Shiraki A, Kotooka N, Komoda H, Hirase T, Oyama J, Node K. Pentraxin-3 regulates the inflammatory activity of macrophages. Biochem Biophys Rep. 2016;5:290-295 pubmed publisher
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, et al. Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection. Exp Ther Med. 2017;14:1846-1852 pubmed publisher
Mihajlovic M, van den Heuvel L, Hoenderop J, Jansen J, Wilmer M, Westheim A, et al. Allostimulatory capacity of conditionally immortalized proximal tubule cell lines for bioartificial kidney application. Sci Rep. 2017;7:7103 pubmed publisher
Stecher C, Battin C, Leitner J, Zettl M, Grabmeier Pfistershammer K, Höller C, et al. PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells. Front Immunol. 2017;8:572 pubmed publisher
Rizkallah G, Alais S, Futsch N, Tanaka Y, Journo C, Mahieux R, et al. Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells. PLoS Pathog. 2017;13:e1006353 pubmed publisher
Sontag S, Förster M, Qin J, Wanek P, Mitzka S, Schüler H, et al. Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells. Stem Cells. 2017;35:898-908 pubmed publisher
Je S, Quan H, Na Y, Cho S, Kim B, Seok S. An in vitro model of granuloma-like cell aggregates substantiates early host immune responses against Mycobacterium massiliense infection. Biol Open. 2016;5:1118-27 pubmed publisher
Zhong Y, Yi C. MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3. Biosci Rep. 2016;36: pubmed publisher
McGrath J, Williamson K, Balasubramanian S, Odate S, Arora S, Hatton C, et al. Pharmacological Inhibition of the Histone Lysine Demethylase KDM1A Suppresses the Growth of Multiple Acute Myeloid Leukemia Subtypes. Cancer Res. 2016;76:1975-88 pubmed publisher
Ren X, Li C, Xiong S, Huang Z, Wang J, Wang H. Antibodies to P-selectin glycoprotein ligand-1 block dendritic cell-mediated enterovirus 71 transmission and prevent virus-induced cells death. Virulence. 2015;6:802-8 pubmed publisher
Zhang L, Liu D, Pu D, Wang Y, Li L, He Y, et al. The role of Toll-like receptor 3 and 4 in regulating the function of mesenchymal stem cells isolated from umbilical cord. Int J Mol Med. 2015;35:1003-10 pubmed publisher
Waschbisch A, Sanderson N, Krumbholz M, Vlad G, Theil D, Schwab S, et al. Interferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients. PLoS ONE. 2014;9:e115488 pubmed publisher
Lopes D, Fernandes J, Cardoso T, Bafica A, Oliveira S, Carvalho E, et al. Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients. Biomed Res Int. 2014;2014:743069 pubmed publisher
Osman A, Bhuyan F, Hashimoto M, Nasser H, Maekawa T, Suzu S. M-CSF inhibits anti-HIV-1 activity of IL-32, but they enhance M2-like phenotypes of macrophages. J Immunol. 2014;192:5083-9 pubmed publisher
Moh M, Lorenzini P, Gullo C, Schwarz H. Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling. FASEB J. 2013;27:2957-66 pubmed publisher
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Wege A, Florian C, Ernst W, Zimara N, Schleicher U, Hanses F, et al. Leishmania major infection in humanized mice induces systemic infection and provokes a nonprotective human immune response. PLoS Negl Trop Dis. 2012;6:e1741 pubmed publisher
Schmitt D, O Dee D, Horzempa J, Carlson P, Russo B, Bales J, et al. A Francisella tularensis live vaccine strain that improves stimulation of antigen-presenting cells does not enhance vaccine efficacy. PLoS ONE. 2012;7:e31172 pubmed publisher
Qin Y, Li Y, Liu W, Tian R, Guo Q, Li S, et al. Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells. PLoS ONE. 2011;6:e27609 pubmed publisher
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Wu Q, Jinde K, Endoh M, Sakai H. Clinical significance of costimulatory molecules CD80/CD86 expression in IgA nephropathy. Kidney Int. 2004;65:888-96 pubmed
Kapsogeorgou E, Moutsopoulos H, Manoussakis M. Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4. J Immunol. 2001;166:3107-13 pubmed
Jeannin P, Delneste Y, Lecoanet Henchoz S, Gauchat J, Ellis J, Bonnefoy J. CD86 (B7-2) on human B cells. A functional role in proliferation and selective differentiation into IgE- and IgG4-producing cells. J Biol Chem. 1997;272:15613-9 pubmed
Azuma M, Ito D, Yagita H, Okumura K, Phillips J, Lanier L, et al. B70 antigen is a second ligand for CTLA-4 and CD28. Nature. 1993;366:76-9 pubmed
product information
Product Type :
Antibody
Product Name :
CD86 (B7-2) Monoclonal Antibody (IT2.2), PE, eBioscience
Catalog # :
12-0869-41
Quantity :
25 Tests
Price :
US 96.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 5 uL (0.5 ug)/test
Species :
Human
Clone :
IT2.2
Isotype :
IgG2b, kappa
Storage :
4 C, store in dark, DO NOT FREEZE!
Description :
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 uL (0.5 ug)/test
Aliases :
activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA