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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45 Monoclonal Antibody (HI30), PE, eBioscience™
catalog :
12-0459-42
quantity :
100 Tests
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
HI30
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 69
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2b
Liu G, Yu Y, Feng F, Zhu P, Zhang H, Zhang D, et al. Human CD8+CD28- T suppressor cells expanded by common gamma chain (γc) cytokines retain steady allospecific suppressive capacity in vivo. BMC Immunol. 2020;21:23 pubmed publisher
  • flow cytometry; mouse; fig 5
Benyoucef A, Palii C, Wang C, Porter C, Chu A, Dai F, et al. UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia. Genes Dev. 2016;30:508-21 pubmed publisher
Xiang A, Xue M, Ren F, Wang L, Ye Z, Li D, et al. High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration. Oncol Rep. 2020;43:1975-1985 pubmed publisher
Ladinsky M, Khamaikawin W, Jung Y, Lin S, Lam J, An D, et al. Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice. elife. 2019;8: pubmed publisher
Nixon A, Duque A, Yelle N, McLaughlin M, Davoudi S, Pedley N, et al. A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations. Sci Rep. 2019;9:842 pubmed publisher
Yan N, Xu J, Zhao C, Wu Y, Gao F, Li C, et al. Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice. Exp Ther Med. 2018;16:4445-4456 pubmed publisher
Wang Y, Guo L, Feng L, Zhang W, Xiao T, Di X, et al. Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer. Oncol Rep. 2018;39:2147-2159 pubmed publisher
Apostolou P, Ntanovasilis D, Papasotiriou I. Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection. J Biol Res (Thessalon). 2017;24:11 pubmed publisher
Li Y, Brauer P, Singh J, Xhiku S, Yoganathan K, Zuniga Pflucker J, et al. Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis. Stem Cell Reports. 2017;9:779-795 pubmed publisher
Wagner J, Pfannenstiel V, Waldmann A, Bergs J, Brill B, Huenecke S, et al. A Two-Phase Expansion Protocol Combining Interleukin (IL)-15 and IL-21 Improves Natural Killer Cell Proliferation and Cytotoxicity against Rhabdomyosarcoma. Front Immunol. 2017;8:676 pubmed publisher
Fuchi N, Miura K, Doi H, Li T, Masuzaki H. Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders. Sci Rep. 2017;7:46220 pubmed publisher
Syed Khaja A, Toor S, El Salhat H, Faour I, Ul Haq N, Ali B, et al. Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment. Oncotarget. 2017;8:33159-33171 pubmed publisher
Liu Z, Guo W, Zhang D, Pang Y, Shi J, Wan S, et al. Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry. Sci Rep. 2016;6:39808 pubmed publisher
Cuff A, Robertson F, Stegmann K, Pallett L, Maini M, Davidson B, et al. Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation. J Immunol. 2016;197:4283-4291 pubmed
Dowling J, Cai Y, Bertin J, Gough P, Zhang J. Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation. Cell Death Dis. 2016;7:e2379 pubmed publisher
Jin Y, Zhou J, Xu F, Jin B, Cui L, Wang Y, et al. Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia. J Clin Invest. 2016;126:3961-3980 pubmed publisher
Mitchell A, Gray W, Hayek S, Ko Y, Thomas S, Rooney K, et al. Platelets confound the measurement of extracellular miRNA in archived plasma. Sci Rep. 2016;6:32651 pubmed publisher
Yeo T, Tan S, Lim C, Lau D, Chua Y, Krisna S, et al. Microfluidic enrichment for the single cell analysis of circulating tumor cells. Sci Rep. 2016;6:22076 pubmed publisher
Doi H, Kitajima Y, Luo L, Yan C, Tateishi S, Ono Y, et al. Potency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing. Sci Rep. 2016;6:18844 pubmed publisher
Gargus M, Niu C, Vallone J, Binkley J, Rubin D, Shaker A. Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands. Am J Physiol Gastrointest Liver Physiol. 2015;308:G904-23 pubmed
Rönn R, Guibentif C, Moraghebi R, Chaves P, Saxena S, Garcia B, et al. Retinoic acid regulates hematopoietic development from human pluripotent stem cells. Stem Cell Reports. 2015;4:269-81 pubmed publisher
Feng Y, Zhu M, Dangelmajer S, Lee Y, Wijesekera O, Castellanos C, et al. Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer. Cell Death Dis. 2014;5:e1567 pubmed publisher
Griffiths E, Golding M, Srivastava P, Povinelli B, James S, Ford L, et al. Pharmacological targeting of ?-catenin in normal karyotype acute myeloid leukemia blasts. Haematologica. 2015;100:e49-52 pubmed publisher
Wang D, Feng X, Lu L, Konkel J, Zhang H, Chen Z, et al. A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus. Arthritis Rheumatol. 2014;66:2234-45 pubmed publisher
Hope C, Grace B, Pilkington K, Coates P, Bergmann I, Carroll R. The immune phenotype may relate to cancer development in kidney transplant recipients. Kidney Int. 2014;86:175-83 pubmed publisher
Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H. CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells. Br J Haematol. 2014;165:134-44 pubmed publisher
Simard C, Cloutier M, Néron S. Feasibility study: phospho-specific flow cytometry enabling rapid functional analysis of bone marrow samples from patients with multiple myeloma. Cytometry B Clin Cytom. 2013;: pubmed publisher
Hillig T, Nygaard A, Nekiunaite L, Klingelhofer J, Söletormos G. In vitro validation of an ultra-sensitive scanning fluorescence microscope for analysis of circulating tumor cells. APMIS. 2014;122:545-51 pubmed publisher
Carvalho P, Leonor I, Smith B, Dias I, Reis R, Gimble J, et al. Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun starch-polycaprolactone scaffolds enhance bone regeneration: nude mice calvarial defect in vivo study. J Biomed Mater Res A. 2014;102:3102-11 pubmed publisher
Putnam A, Safinia N, Medvec A, Laszkowska M, Wray M, Mintz M, et al. Clinical grade manufacturing of human alloantigen-reactive regulatory T cells for use in transplantation. Am J Transplant. 2013;13:3010-20 pubmed publisher
Lavender K, Pang W, Messer R, Duley A, Race B, Phillips K, et al. BLT-humanized C57BL/6 Rag2-/-?c-/-CD47-/- mice are resistant to GVHD and develop B- and T-cell immunity to HIV infection. Blood. 2013;122:4013-20 pubmed publisher
Melief J, Schuurman K, van de Garde M, Smolders J, van Eijk M, Hamann J, et al. Microglia in normal appearing white matter of multiple sclerosis are alerted but immunosuppressed. Glia. 2013;61:1848-61 pubmed publisher
Murakami M, Horibe H, Iohara K, Hayashi Y, Osako Y, Takei Y, et al. The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential. Biomaterials. 2013;34:9036-47 pubmed publisher
Alvarez Gonzalez C, Duggleby R, Vagaska B, Querol S, Gomez S, Ferretti P, et al. Cord blood Lin(-)CD45(-) embryonic-like stem cells are a heterogeneous population that lack self-renewal capacity. PLoS ONE. 2013;8:e67968 pubmed publisher
Koba C, Haruta M, Matsunaga Y, Matsumura K, Haga E, Sasaki Y, et al. Therapeutic effect of human iPS-cell-derived myeloid cells expressing IFN-? against peritoneally disseminated cancer in xenograft models. PLoS ONE. 2013;8:e67567 pubmed publisher
Bloch O, Crane C, Kaur R, Safaee M, Rutkowski M, Parsa A. Gliomas promote immunosuppression through induction of B7-H1 expression in tumor-associated macrophages. Clin Cancer Res. 2013;19:3165-75 pubmed publisher
Wu S, Ju G, Du T, Zhu Y, Liu G. Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo. PLoS ONE. 2013;8:e61366 pubmed publisher
Schmitt N, Bustamante J, Bourdery L, Bentebibel S, Boisson Dupuis S, Hamlin F, et al. IL-12 receptor ?1 deficiency alters in vivo T follicular helper cell response in humans. Blood. 2013;121:3375-85 pubmed publisher
Raki M, Beitnes A, Lundin K, Jahnsen J, Jahnsen F, Sollid L. Plasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion. Mucosal Immunol. 2013;6:985-92 pubmed publisher
Bruck F, Belle L, Lechanteur C, de Leval L, Hannon M, Dubois S, et al. Impact of bone marrow-derived mesenchymal stromal cells on experimental xenogeneic graft-versus-host disease. Cytotherapy. 2013;15:267-79 pubmed publisher
Cosgrove C, Ussher J, Rauch A, Gärtner K, Kurioka A, Hühn M, et al. Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection. Blood. 2013;121:951-61 pubmed publisher
Ali N, Flutter B, Sanchez Rodriguez R, Sharif Paghaleh E, Barber L, Lombardi G, et al. Xenogeneic graft-versus-host-disease in NOD-scid IL-2Rγnull mice display a T-effector memory phenotype. PLoS ONE. 2012;7:e44219 pubmed publisher
Liu R, Ratajczak M. Enumeration of very small embryonic-like stem cells in peripheral blood. Methods Mol Biol. 2012;904:207-19 pubmed publisher
Melief J, Koning N, Schuurman K, van de Garde M, Smolders J, Hoek R, et al. Phenotyping primary human microglia: tight regulation of LPS responsiveness. Glia. 2012;60:1506-17 pubmed publisher
Danova Alt R, Heider A, Egger D, Cross M, Alt R. Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics. PLoS ONE. 2012;7:e34899 pubmed publisher
Sage P, Varghese L, Martinelli R, Sciuto T, Kamei M, Dvorak A, et al. Antigen recognition is facilitated by invadosome-like protrusions formed by memory/effector T cells. J Immunol. 2012;188:3686-99 pubmed publisher
Beitnes A, Raki M, Brottveit M, Lundin K, Jahnsen F, Sollid L. Rapid accumulation of CD14+CD11c+ dendritic cells in gut mucosa of celiac disease after in vivo gluten challenge. PLoS ONE. 2012;7:e33556 pubmed publisher
Svensson J, Jenmalm M, Matussek A, Geffers R, Berg G, Ernerudh J. Macrophages at the fetal-maternal interface express markers of alternative activation and are induced by M-CSF and IL-10. J Immunol. 2011;187:3671-82 pubmed publisher
Claassen M, de Knegt R, Janssen H, Boonstra A. Retention of CD4+ CD25+ FoxP3+ regulatory T cells in the liver after therapy-induced hepatitis C virus eradication in humans. J Virol. 2011;85:5323-30 pubmed publisher
Beitnes A, Raki M, Lundin K, Jahnsen J, Sollid L, Jahnsen F. Density of CD163+ CD11c+ dendritic cells increases and CD103+ dendritic cells decreases in the coeliac lesion. Scand J Immunol. 2011;74:186-94 pubmed publisher
Nguyen S, Beziat V, Norol F, Uzunov M, Trebeden Negre H, Azar N, et al. Infusion of allogeneic natural killer cells in a patient with acute myeloid leukemia in relapse after haploidentical hematopoietic stem cell transplantation. Transfusion. 2011;51:1769-78 pubmed publisher
Zhang Z, Shively J. Generation of novel bone forming cells (monoosteophils) from the cathelicidin-derived peptide LL-37 treated monocytes. PLoS ONE. 2010;5:e13985 pubmed publisher
Song J, Willinger T, Rongvaux A, Eynon E, Stevens S, Manz M, et al. A mouse model for the human pathogen Salmonella typhi. Cell Host Microbe. 2010;8:369-76 pubmed publisher
Kreisel D, Richardson S, Li W, Lin X, Kornfeld C, Sugimoto S, et al. Cutting edge: MHC class II expression by pulmonary nonhematopoietic cells plays a critical role in controlling local inflammatory responses. J Immunol. 2010;185:3809-13 pubmed publisher
Freeman C, Han M, Martinez F, Murray S, Liu L, Chensue S, et al. Cytotoxic potential of lung CD8(+) T cells increases with chronic obstructive pulmonary disease severity and with in vitro stimulation by IL-18 or IL-15. J Immunol. 2010;184:6504-13 pubmed publisher
Yu G, Wu X, Dietrich M, Polk P, Scott L, Ptitsyn A, et al. Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes. Cytotherapy. 2010;12:538-46 pubmed publisher
Claassen M, de Knegt R, Tilanus H, Janssen H, Boonstra A. Abundant numbers of regulatory T cells localize to the liver of chronic hepatitis C infected patients and limit the extent of fibrosis. J Hepatol. 2010;52:315-21 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD45 Monoclonal Antibody (HI30), PE, eBioscience™
Catalog # :
12-0459-42
Quantity :
100 Tests
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 5 µL (0.06 µg)/test
Species :
Human
Clone :
HI30
Isotype :
IgG1, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 µL (0.06 µg)/test
Aliases :
B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA