ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9)
Invitrogen
catalog: MA3-060
mouse monoclonal (1D9)
reactivity: Chinese hamsters, human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 22

Immunofluorescent analysis of ADP-Ribosylation Factor using ADP-Ribosylation Factor Monoclonal Antibody (1D9) (Product# MA3-060 ) shows staining in MCF-7 Cells. ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing ADP-Ribosylation Factor (Product# MA3-060 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
quantity: 100 uL
price: US 491.00
to the supplier
ARF6 Polyclonal Antibody
Invitrogen
catalog: PA1-093
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 6

Western blot analysis of Arf6 was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Arf6 (Product # PA1-093) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product #31460) at a dilution of 1:10000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34080).
quantity: 100 ug
price: US 451.00
to the supplier
ARF6 Polyclonal Antibody
Invitrogen
catalog: PA1-093X
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 6

Western blot analysis of Arf6 was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Arf6 (Product # PA1-093) at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product #31460) at a dilution of 1:10000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34080).

Immunofluorescent analysis of Arf6 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with an Arf6 polyclonal antibody (Product # PA1-093) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
quantity: 20 uL
price: US 166.00
to the supplier
ARF6 Monoclonal Antibody (6ARF01)
Invitrogen
catalog: MA5-11795
mouse monoclonal (6ARF01)
reactivity: human, mouse
application: western blot, immunocytochemistry
citations: 1
quantity: 500 uL
price: US 480.00
to the supplier
ARF1/ARF2/ARF3/ARF4 Polyclonal Antibody
Invitrogen
catalog: PA5-18627
domestic goat polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, flow cytometry

Western Blot staining of HepG2 cell lysate using PA5-18627 at a concentration of 1.0 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100 ug
price: US 464.00
to the supplier
ARF6 Recombinant Rabbit Monoclonal Antibody (JF101-2)
Invitrogen
catalog: MA5-32558
domestic rabbit recombinant (JF101-2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
ARF6 Polyclonal Antibody
Invitrogen
catalog: PA5-85348
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
ARF6 Recombinant Rabbit Monoclonal Antibody (ARC0617)
Invitrogen
catalog: MA5-35119
domestic rabbit recombinant (ARC0617)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
quantity: 100 uL
price: US 474.00
to the supplier
ARF6 Recombinant Rabbit Monoclonal Antibody (23GB3160)
Invitrogen
catalog: MA5-53390
domestic rabbit recombinant (23GB3160)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
quantity: 100 uL
price: US 539.00
to the supplier
ARF6 Recombinant Rabbit Monoclonal Antibody (23GB3160)
Invitrogen
catalog: MA5-53391
domestic rabbit recombinant (23GB3160)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
quantity: 20 uL
price: US 199.00
to the supplier