RAGE Polyclonal Antibody
Invitrogen
catalog: PA1-075
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 9

Immunofluorescent analysis of RAGE in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAGE polyclonal antibody (Product # PA1-075) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). RAGE staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue . To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE (PA1-075) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100 ug
price: US 471.00
to the supplier
RAGE Polyclonal Antibody
Invitrogen
catalog: PA5-24787
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 2

Western blot analysis of AGER in mouse lung tissue lysates using 35ug per lane of antibody.

Immunohistochemical analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
quantity: 400 uL
price: US 464.00
to the supplier
RAGE Polyclonal Antibody
Invitrogen
catalog: PA5-78736
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 430.00
to the supplier
RAGE Polyclonal Antibody
Invitrogen
catalog: PA5-78735
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity: 100 ug
price: US 430.00
to the supplier
RAGE Recombinant Rabbit Monoclonal Antibody (005)
Invitrogen
catalog: MA5-30062
domestic rabbit recombinant (5)
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 410.00
to the supplier
RAGE Polyclonal Antibody
Invitrogen
catalog: PA5-89548
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 464.00
to the supplier
RAGE Recombinant Rabbit Monoclonal Antibody (JF0975)
Invitrogen
catalog: MA5-41206
domestic rabbit recombinant (JF0975)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
RAGE Monoclonal Antibody (5C6C1)
Invitrogen
catalog: MA5-49253
mouse monoclonal (5C6C1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 404.00
to the supplier