mouse monoclonal (2CHK01 (DCS-293))
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
citations: 4

quantity: 500 uL
price: US 552.00
to the supplier
mouse monoclonal (ebchk2)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
citations: 4
quantity: 100 Tests
price: US 441.00
to the supplier
Phospho-Chk2 (Thr68) Polyclonal Antibody
Invitrogen
catalog: PA5-104715
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 3
quantity: 100 uL
price: US 454.00
to the supplier
Phospho-CHK2 (Ser516) Polyclonal Antibody
Invitrogen
catalog: PA5-17571
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-17618
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
quantity: 100 uL
price: US 404.00
to the supplier
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-CHK2 (Thr68) Polyclonal Antibody
Invitrogen
catalog: PA5-17818
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

Immunofluorescent analysis of Phospho-Chk2 pThr68 (green) in HeLa cells either left untreated (left panel) or treated with 25ug/ml anisomycin for 30 minutes (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-Chk2 pThr68 polyclonal antibody (Product # PA5-17818) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

Flow cytometric analysis of phospho-Chk2 pThr68 on untreated (blue histogram) or UV-treated (green histogram) HeLa cells. Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Chk2 pThr68 polyclonal antibody (Product # PA5-17818) at a 1:800 dilution for 1 hour at room temperature. Cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.

(A) Chk2 phosphorylation: Chk2 is phosphorylated by ATM kinase in response to DNA damage and is important in DNA damage induced checkpoint signaling. A549 cells were treated with vehicle (DMSO) or 50 µM etoposide for 3 hrs. After staining cells with Cellomics Chk2 activation kit, images were acquired and analyzed. Z-factor for phospho-Chk2 is 0.49 ± 0.03 and %COV is 11.2 ± 0.4. EC50 of etoposide treatment for Chk2 phosphorylation is 1.8 µM.
quantity: 100 uL
price: US 522.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-19971
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry


quantity: 100 ug
price: US 464.00
to the supplier
CHK2 Monoclonal Antibody (73C175.1.1)
Invitrogen
catalog: MA1-41250
mouse monoclonal (73C175.1.1)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 464.00
to the supplier
Phospho-CHK2 (Ser19) Polyclonal Antibody
Invitrogen
catalog: PA5-17731
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-CHK2 (Thr387) Polyclonal Antibody
Invitrogen
catalog: PA5-36903
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-CHK2 (Thr432) Polyclonal Antibody
Invitrogen
catalog: PA5-37339
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit recombinant (45H21L9)
reactivity: human
application: western blot, immunocytochemistry
quantity: 100 ug
price: US 520.00
to the supplier
Phospho-CHK2 (Thr432) Polyclonal Antibody
Invitrogen
catalog: PA5-64492
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-CHK2 (Thr68) Polyclonal Antibody
Invitrogen
catalog: PA5-78361
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 481.00
to the supplier
Phospho-CHK2 (Ser19) Polyclonal Antibody
Invitrogen
catalog: PA5-78360
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity: 100 uL
price: US 474.00
to the supplier
domestic rabbit recombinant (Chk2T68-D12)
reactivity: human, mouse
application: western blot, flow cytometry
quantity: 200 uL
price: US 480.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-79041
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 410.00
to the supplier
CHK2 Monoclonal Antibody (8F12)
Invitrogen
catalog: MA5-31595
mouse monoclonal (8F12)
reactivity: human
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 474.00
to the supplier
domestic rabbit recombinant (SC604)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 474.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-81982
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 474.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-85360
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 464.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-86222
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-86125
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-94948
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 ug
price: US 464.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-87441
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
quantity: 100 uL
price: US 464.00
to the supplier
CHK2 Polyclonal Antibody
Invitrogen
catalog: PA5-96068
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit recombinant (2W5N3)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 474.00
to the supplier
Phospho-Chk2 (Thr387) Polyclonal Antibody
Invitrogen
catalog: PA5-104713
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 454.00
to the supplier
Phospho-Chk2 (Thr383) Polyclonal Antibody
Invitrogen
catalog: PA5-104714
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 454.00
to the supplier
Phospho-Chk2 (Ser19) Polyclonal Antibody
Invitrogen
catalog: PA5-105766
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-Chk2 (Ser516) Polyclonal Antibody
Invitrogen
catalog: PA5-99342
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit recombinant (Chk2T68-D12)
reactivity: human, mouse
application: western blot, flow cytometry
quantity: 20 uL
price: US 319.00
to the supplier
domestic rabbit recombinant (Chk2T68-D12)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
quantity: 100 Tests
price: US 484.00
to the supplier
domestic rabbit recombinant (Chk2T68-D12)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
quantity: 100 Tests
price: US 474.00
to the supplier
domestic rabbit recombinant (Chk2T68-D12)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
quantity: 100 Tests
price: US 484.00
to the supplier
domestic rabbit recombinant (Chk2T68-D12)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
quantity: 10 Tests
price: US 197.00
to the supplier
domestic rabbit recombinant (Chk2T68-D12)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
quantity: 10 Tests
price: US 197.00
to the supplier
domestic rabbit recombinant (Chk2T68-D12)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
quantity: 10 Tests
price: US 197.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-CHK2 (Ser516) Polyclonal Antibody
Invitrogen
catalog: PA5-117206
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 474.00
to the supplier
Phospho-CHK2 (Thr432) Polyclonal Antibody
Invitrogen
catalog: PA5-118609
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 434.00
to the supplier
Phospho-CHK2 (Ser379) Polyclonal Antibody
Invitrogen
catalog: PA5-118750
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 434.00
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity: 100 uL
price: US 460.00
to the supplier
domestic rabbit recombinant (23GB3460)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
quantity: 100 uL
price: US 539.00
to the supplier
domestic rabbit recombinant (23GB3460)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
quantity: 20 uL
price: US 199.00
to the supplier
domestic rabbit recombinant (7R1S5)
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 419.00
to the supplier