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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Perforin Monoclonal Antibody (dG9 (delta G9)), FITC, eBioscience
catalog :
11-9994-42
quantity :
100 Tests
price :
US 333
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
dG9 (delta G9)
reactivity :
human, pigs
application :
immunohistochemistry, flow cytometry
more info or order :
Invitrogen product webpage
citations: 24
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 3
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
  • flow cytometry; human
Pegram H, Purdon T, van Leeuwen D, Curran K, Giralt S, Barker J, et al. IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia. Leukemia. 2015;29:415-22 pubmed publisher
Galat Y, Du Y, Perepitchka M, Li X, Balyasnikova I, Tse W, et al. In vitro vascular differentiation system efficiently produces natural killer cells for cancer immunotherapies. Oncoimmunology. 2023;12:2240670 pubmed publisher
Cascone T, Leung C, Weissferdt A, Pataer A, Carter B, Godoy M, et al. Neoadjuvant chemotherapy plus nivolumab with or without ipilimumab in operable non-small cell lung cancer: the phase 2 platform NEOSTAR trial. Nat Med. 2023;29:593-604 pubmed publisher
Lao L, Zeng W, Huang P, Chen H, Jia Z, Wang P, et al. CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance. Cancer Immunol Res. 2023;11:320-338 pubmed publisher
Zhang M, Chong K, Chen Z, Guo H, Liu Y, Kang Y, et al. Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes. JCI Insight. 2023;8: pubmed publisher
Liu X, Jin S, Hu S, Li R, Pan H, Liu Y, et al. Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma. Nat Commun. 2022;13:1158 pubmed publisher
Li H, Yang Z, Yang X, Zhang F, Wang J, Wu Z, et al. LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma. Cell Death Dis. 2022;13:109 pubmed publisher
Rha M, Jeong H, Ko J, Choi S, Seo I, Lee J, et al. PD-1-Expressing SARS-CoV-2-Specific CD8+ T Cells Are Not Exhausted, but Functional in Patients with COVID-19. Immunity. 2021;54:44-52.e3 pubmed publisher
Billiet L, Goetgeluk G, Bonte S, De Munter S, De Cock L, Pille M, et al. Human Thymic CD10+ PD-1+ Intraepithelial Lymphocyte Precursors Acquire Interleukin-15 Responsiveness at the CD1a- CD95+ CD28- CCR7- Developmental Stage. Int J Mol Sci. 2020;21: pubmed publisher
Mitchell K, Diao L, Karpinets T, Negrao M, Tran H, Parra E, et al. Neutrophil expansion defines an immunoinhibitory peripheral and intratumoral inflammatory milieu in resected non-small cell lung cancer: a descriptive analysis of a prospectively immunoprofiled cohort. J Immunother Cancer. 2020;8: pubmed publisher
Rotolo A, Caputo V, Holubova M, Baxan N, Dubois O, Chaudhry M, et al. Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting. Cancer Cell. 2018;34:596-610.e11 pubmed publisher
Seebauer C, Brunner S, Glockzin G, Piso P, Ruemmele P, Schlitt H, et al. Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells. Oncoimmunology. 2016;5:e1242543 pubmed publisher
Oja A, Vieira Braga F, Remmerswaal E, Kragten N, Hertoghs K, Zuo J, et al. The Transcription Factor Hobit Identifies Human Cytotoxic CD4+ T Cells. Front Immunol. 2017;8:325 pubmed publisher
Su S, Liao J, Liu J, Huang D, He C, Chen F, et al. Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Res. 2017;27:461-482 pubmed publisher
Collins A, Rothman N, Liu K, Reiner S. Eomesodermin and T-bet mark developmentally distinct human natural killer cells. JCI Insight. 2017;2:e90063 pubmed publisher
Srivastava R, Khan A, Garg S, Syed S, Furness J, Vahed H, et al. Human Asymptomatic Epitopes Identified from the Herpes Simplex Virus Tegument Protein VP13/14 (UL47) Preferentially Recall Polyfunctional Effector Memory CD44high CD62Llow CD8+ TEM Cells and Protect Humanized HLA-A*02:01 Transgenic Mice against Ocula. J Virol. 2017;91: pubmed publisher
Braun M, Ress M, Yoo Y, Scholz C, Eyrich M, Schlegel P, et al. IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing. Oncoimmunology. 2016;5:e1188245 pubmed publisher
Ghosh S, Taylor A, Chin M, Huang H, Conery A, Mertz J, et al. Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition. J Biol Chem. 2016;291:13014-27 pubmed publisher
Ivarsson M, Loh L, Marquardt N, Kekäläinen E, Berglin L, Björkström N, et al. Differentiation and functional regulation of human fetal NK cells. J Clin Invest. 2013;123:3889-901 pubmed publisher
Barnig C, Cernadas M, Dutile S, Liu X, Perrella M, Kazani S, et al. Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma. Sci Transl Med. 2013;5:174ra26 pubmed publisher
Yu Y, Zitzner J, Houlihan J, Herrera N, Xu L, Miller J, et al. Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells. PLoS ONE. 2011;6:e28948 pubmed publisher
Kleijwegt F, Laban S, Duinkerken G, Joosten A, Koeleman B, Nikolic T, et al. Transfer of regulatory properties from tolerogenic to proinflammatory dendritic cells via induced autoreactive regulatory T cells. J Immunol. 2011;187:6357-64 pubmed publisher
Burt B, Plitas G, Zhao Z, Bamboat Z, Nguyen H, Dupont B, et al. The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors. J Immunol. 2009;183:1789-96 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Perforin Monoclonal Antibody (dG9 (delta G9)), FITC, eBioscience
Catalog # :
11-9994-42
Quantity :
100 Tests
Price :
US 333
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human, Porcine
Applications :
Flow Cytometry: 5 µL (0.125 µg)/test, Immunohistochemistry: Assay-Dependent
Species :
Human, Porcine
Clone :
dG9 (delta G9)
Isotype :
IgG2b, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 µL (0.125 µg)/test, Immunohistochemistry: Assay-Dependent
Aliases :
Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1; Prf-1; PRF1 (pore forming protein 1); RATCYTA; RP11-710A11.3
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA