product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HELIOS Monoclonal Antibody (22F6), FITC, eBioscience™
catalog :
11-9883-80
quantity :
25 µg
price :
US 309.00
clonality :
monoclonal
host :
hamsters
conjugate :
FITC
clone name :
22F6
reactivity :
human, mouse, dogs
application :
flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s2a
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed publisher
  • flow cytometry; mouse; fig s3e
Ansa Addo E, Zhang Y, Yang Y, Hussey G, Howley B, Salem M, et al. Membrane-organizing protein moesin controls Treg differentiation and antitumor immunity via TGF-β signaling. J Clin Invest. 2017;127:1321-1337 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 4a
Barthels C, Ogrinc A, Steyer V, Meier S, Simon F, Wimmer M, et al. CD40-signalling abrogates induction of RORγt+ Treg cells by intestinal CD103+ DCs and causes fatal colitis. Nat Commun. 2017;8:14715 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1c
He R, Hou S, Liu C, Zhang A, Bai Q, Han M, et al. Follicular CXCR5- expressing CD8(+) T cells curtail chronic viral infection. Nature. 2016;537:412-428 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Saha A, O Connor R, Thangavelu G, Lovitch S, Dandamudi D, Wilson C, et al. Programmed death ligand-1 expression on donor T cells drives graft-versus-host disease lethality. J Clin Invest. 2016;126:2642-60 pubmed publisher
Chien C, Yu H, Chen S, Chiang B. Characterization of c-Maf+Foxp3- Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells. Sci Rep. 2017;7:46348 pubmed publisher
Jhala G, Chee J, Trivedi P, Selck C, Gurzov E, Graham K, et al. Perinatal tolerance to proinsulin is sufficient to prevent autoimmune diabetes. JCI Insight. 2016;1:e86065 pubmed publisher
Sebastian M, Lopez Ocasio M, Metidji A, RIEDER S, Shevach E, Thornton A. Helios Controls a Limited Subset of Regulatory T Cell Functions. J Immunol. 2016;196:144-55 pubmed publisher
Dilek N, Poirier N, Hulin P, Coulon F, Mary C, Ville S, et al. Targeting CD28, CTLA-4 and PD-L1 costimulation differentially controls immune synapses and function of human regulatory and conventional T-cells. PLoS ONE. 2013;8:e83139 pubmed publisher
Eneljung T, Tengvall S, Jirholt P, Henningsson L, Holmdahl R, Gustafsson K, et al. Antigen-specific gene therapy after immunisation reduces the severity of collagen-induced arthritis. Clin Dev Immunol. 2013;2013:345092 pubmed publisher
Dingle B, Liu Y, Fatheree N, Min J, Rhoads J, Tran D. FoxP3? regulatory T cells attenuate experimental necrotizing enterocolitis. PLoS ONE. 2013;8:e82963 pubmed publisher
van der Merwe M, Abdelsamed H, Seth A, Ong T, Vogel P, Pillai A. Recipient myeloid-derived immunomodulatory cells induce PD-1 ligand-dependent donor CD4+Foxp3+ regulatory T cell proliferation and donor-recipient immune tolerance after murine nonmyeloablative bone marrow transplantation. J Immunol. 2013;191:5764-76 pubmed publisher
Yamamoto Taguchi N, Satou Y, Miyazato P, Ohshima K, Nakagawa M, Katagiri K, et al. HTLV-1 bZIP factor induces inflammation through labile Foxp3 expression. PLoS Pathog. 2013;9:e1003630 pubmed publisher
Peters J, Koenen H, Fasse E, Tijssen H, Ijzermans J, Groenen P, et al. Human secondary lymphoid organs typically contain polyclonally-activated proliferating regulatory T cells. Blood. 2013;122:2213-23 pubmed publisher
Tsai S, Serra P, Clemente Casares X, Slattery R, Santamaria P. Dendritic cell-dependent in vivo generation of autoregulatory T cells by antidiabetogenic MHC class II. J Immunol. 2013;191:70-82 pubmed publisher
Podojil J, Liu L, Marshall S, Chiang M, Goings G, Chen L, et al. B7-H4Ig inhibits mouse and human T-cell function and treats EAE via IL-10/Treg-dependent mechanisms. J Autoimmun. 2013;44:71-81 pubmed publisher
Macdonald K, Han J, Himmel M, Huang Q, Kan B, Campbell A, et al. Response to comment on "helios+ and helios- cells coexist within the natural FOXP3+ T regulatory cell subset in humans". J Immunol. 2013;190:4440-1 pubmed publisher
Stumpf M, Zhou X, Bluestone J. The B7-independent isoform of CTLA-4 functions to regulate autoimmune diabetes. J Immunol. 2013;190:961-9 pubmed publisher
Baine I, Basu S, Ames R, Sellers R, Macian F. Helios induces epigenetic silencing of IL2 gene expression in regulatory T cells. J Immunol. 2013;190:1008-16 pubmed publisher
Alexander T, Sattler A, Templin L, Kohler S, Groß C, Meisel A, et al. Foxp3+ Helios+ regulatory T cells are expanded in active systemic lupus erythematosus. Ann Rheum Dis. 2013;72:1549-58 pubmed publisher
Sawant D, Sehra S, Nguyen E, Jadhav R, Englert K, Shinnakasu R, et al. Bcl6 controls the Th2 inflammatory activity of regulatory T cells by repressing Gata3 function. J Immunol. 2012;189:4759-69 pubmed publisher
Schlenner S, Weigmann B, Ruan Q, Chen Y, von Boehmer H. Smad3 binding to the foxp3 enhancer is dispensable for the development of regulatory T cells with the exception of the gut. J Exp Med. 2012;209:1529-35 pubmed publisher
Akhmetzyanova I, Zelinskyy G, Schimmer S, Brandau S, Altenhoff P, Sparwasser T, et al. Tumor-specific CD4+ T cells develop cytotoxic activity and eliminate virus-induced tumor cells in the absence of regulatory T cells. Cancer Immunol Immunother. 2013;62:257-71 pubmed publisher
Reynolds L, Maizels R. Cutting edge: in the absence of TGF-? signaling in T cells, fewer CD103+ regulatory T cells develop, but exuberant IFN-? production renders mice more susceptible to helminth infection. J Immunol. 2012;189:1113-7 pubmed publisher
McGovern J, Nguyen D, Notley C, Mauri C, Isenberg D, Ehrenstein M. Th17 cells are restrained by Treg cells via the inhibition of interleukin-6 in patients with rheumatoid arthritis responding to anti-tumor necrosis factor antibody therapy. Arthritis Rheum. 2012;64:3129-38 pubmed publisher
Wang C, Lee J, Kim C. Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch. PLoS ONE. 2012;7:e30793 pubmed publisher
Gottschalk R, Corse E, Allison J. Expression of Helios in peripherally induced Foxp3+ regulatory T cells. J Immunol. 2012;188:976-80 pubmed publisher
Schwele S, Fischer A, Brestrich G, Wlodarski M, Wagner L, Schmueck M, et al. Cytomegalovirus-specific regulatory and effector T cells share TCR clonality--possible relation to repetitive CMV infections. Am J Transplant. 2012;12:669-81 pubmed publisher
Zeng C, Shi X, Zhang B, Liu H, Zhang L, Ding W, et al. The imbalance of Th17/Th1/Tregs in patients with type 2 diabetes: relationship with metabolic factors and complications. J Mol Med (Berl). 2012;90:175-86 pubmed publisher
Wainwright D, Sengupta S, Han Y, Lesniak M. Thymus-derived rather than tumor-induced regulatory T cells predominate in brain tumors. Neuro Oncol. 2011;13:1308-23 pubmed publisher
Onoe T, Kalscheuer H, Danzl N, Chittenden M, Zhao G, Yang Y, et al. Human natural regulatory T cell development, suppressive function, and postthymic maturation in a humanized mouse model. J Immunol. 2011;187:3895-903 pubmed publisher
Weiss K, Christiaansen A, Fulton R, Meyerholz D, Varga S. Multiple CD4+ T cell subsets produce immunomodulatory IL-10 during respiratory syncytial virus infection. J Immunol. 2011;187:3145-54 pubmed publisher
Penaranda C, Tang Q, Bluestone J. Anti-CD3 therapy promotes tolerance by selectively depleting pathogenic cells while preserving regulatory T cells. J Immunol. 2011;187:2015-22 pubmed publisher
Elkord E, Sharma S, Burt D, Hawkins R. Expanded subpopulation of FoxP3+ T regulatory cells in renal cell carcinoma co-express Helios, indicating they could be derived from natural but not induced Tregs. Clin Immunol. 2011;140:218-22 pubmed publisher
McClymont S, Putnam A, Lee M, Esensten J, Liu W, Hulme M, et al. Plasticity of human regulatory T cells in healthy subjects and patients with type 1 diabetes. J Immunol. 2011;186:3918-26 pubmed publisher
Bhagwat A. Comment on "Deoxyuridine triphosphate incorporation during somatic hypermutation of mouse VkOx genes after immunization with phenyloxazolone". J Immunol. 2010;185:7130-1; author reply 7131 pubmed publisher
Verhagen J, Wraith D. Comment on "Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3+ T regulatory cells". J Immunol. 2010;185:7129; author reply 7130 pubmed publisher
product information
Product Type :
Antibody
Product Name :
HELIOS Monoclonal Antibody (22F6), FITC, eBioscience™
Catalog # :
11-9883-80
Quantity :
25 µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Armenian Hamster
Reactivity :
Canine, Human, Mouse
Applications :
Flow Cytometry: 0.125 µg/test
Species :
Canine, Human, Mouse
Clone :
22F6
Isotype :
IgG
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.125 µg/test
Aliases :
A730095J18Rik; ANF1A2; Helios; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); ikaros family zinc finger protein 2; Ikzf2; Zfpn1a2; zinc finger DNA binding protein Helios; zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; Znfn1a2
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA