CD45 Monoclonal Antibody (2D1), FITC, eBioscience | Invitrogen 11-9459-42 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD45 Monoclonal Antibody (2D1), FITC, eBioscience

catalog :

11-9459-42

quantity :

100 Tests

price :

US 277.00

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

2D1

reactivity :

human, mouse

application :

immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4a	Zhang B, Nguyen L, Li L, Zhao D, Kumar B, Wu H, et al. Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia. Nat Med. 2018;24:450-462 pubmed publisher
flow cytometry; human; fig s1d	Su S, Chen J, Yao H, Liu J, Yu S, Lao L, et al. CD10+GPR77+ Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness. Cell. 2018;172:841-856.e16 pubmed publisher
flow cytometry; human; loading ...; fig 1b	Zhu N, Wang H, Wang B, Wei J, Shan W, Feng J, et al. A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells. Stem Cells Int. 2016;2016:5687589 pubmed publisher
flow cytometry; human; fig s4	Fong C, Gilan O, Lam E, Rubin A, Ftouni S, Tyler D, et al. BET inhibitor resistance emerges from leukaemia stem cells. Nature. 2015;525:538-42 pubmed publisher
flow cytometry; human; fig s4	James S, Fox J, Afsari F, Lee J, Clough S, Knight C, et al. Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes. Stem Cell Reports. 2015;4:1004-15 pubmed publisher
	Hua J, Jiao T, Qiao Y, Zhang S, Xiao T, Zhang Y, et al. Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34+ hematopoietic stem/progenitor cells. Ann Transl Med. 2023;11:62 pubmed publisher
	Mu R, Chen B, Bi B, Yu H, Liu J, Li J, et al. LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling. Int J Med Sci. 2022;19:1307-1319 pubmed publisher
	Oyer J, Croom Perez T, Dieffenthaller T, Robles Carillo L, Gitto S, Altomare D, et al. Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo. Front Immunol. 2022;13:861681 pubmed publisher
	Kenswil K, Pisterzi P, Feyen J, Ter Borg M, Rombouts E, Braakman E, et al. Immune composition and its association with hematologic recovery after chemotherapeutic injury in acute myeloid leukemia. Exp Hematol. 2022;105:32-38.e2 pubmed publisher
	Guti xe9 rrez D, Rouabhia M, Ortiz J, Gaviria D, Alfonso C, Mu xf1 oz A, et al. Low-Level Laser Irradiation Promotes Proliferation and Differentiation on Apical Papilla Stem Cells. J Lasers Med Sci. 2021;12:e75 pubmed publisher
	Jiang W, Zhang J, Zhang X, Fan C, Huang J. VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway. Bioengineered. 2021;12:10264-10284 pubmed publisher
	Xiao K, Yang L, Xie W, Gao X, Huang R, Xie M. Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels. Exp Ther Med. 2021;22:736 pubmed publisher
	Bazié W, Boucher J, Vitry J, Goyer B, Routy J, Tremblay C, et al. Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV. Pathog Immun. 2021;6:1-28 pubmed publisher
	Foster D, Marshall C, Gulati G, Chinta M, Nguyen A, Salhotra A, et al. Elucidating the fundamental fibrotic processes driving abdominal adhesion formation. Nat Commun. 2020;11:4061 pubmed publisher
	Singh S, Homad L, Akins N, Stoffers C, Lackhar S, Malhi H, et al. Neutralizing Antibodies Protect against Oral Transmission of Lymphocryptovirus. Cell Rep Med. 2020;1: pubmed publisher
	Sun L, Zhu W, Zhao P, Zhang J, Lu Y, Zhu Y, et al. Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair. Front Cell Dev Biol. 2020;8:263 pubmed publisher
	Guedan S, Madar A, Casado Medrano V, Shaw C, Wing A, Liu F, et al. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability. J Clin Invest. 2020;130:3087-3097 pubmed publisher
	Yan D, Tang B, Yan L, Zhang L, Miao M, Chen X, et al. Sodium Selenite Improves The Therapeutic Effect Of BMSCs Via Promoting The Proliferation And Differentiation, Thereby Promoting The Hematopoietic Factors. Onco Targets Ther. 2019;12:9685-9696 pubmed publisher
	Fan C, Ji Q, Zhang C, Xu S, Sun H, Li Z. TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production. Mol Med Rep. 2019;20:3123-3130 pubmed publisher
	Bell C, Fennell K, Chan Y, Rambow F, Yeung M, Vassiliadis D, et al. Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia. Nat Commun. 2019;10:2723 pubmed publisher
	Sun Y, Ren Y, Yang F, He Y, Liang S, Guan L, et al. High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment. Biol Open. 2019;8: pubmed publisher
	Hakimi A, Voss M, Kuo F, Sanchez A, Liu M, Nixon B, et al. Transcriptomic Profiling of the Tumor Microenvironment Reveals Distinct Subgroups of Clear Cell Renal Cell Cancer: Data from a Randomized Phase III Trial. Cancer Discov. 2019;9:510-525 pubmed publisher
	Xiao L, Xu S, Wang X, Jin Z, Wang J, Yang B, et al. Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage. Mol Med Rep. 2018;18:5751-5759 pubmed publisher
	Ormel P, Vieira de Sá R, van Bodegraven E, Karst H, Harschnitz O, Sneeboer M, et al. Microglia innately develop within cerebral organoids. Nat Commun. 2018;9:4167 pubmed publisher
	Li X, Ma R, Gu Q, Liang L, Wang L, Zhang Y, et al. A fully defined static suspension culture system for large-scale human embryonic stem cell production. Cell Death Dis. 2018;9:892 pubmed publisher
	Narasimhan P, Akabas L, Tariq S, Huda N, Bennuru S, Sabzevari H, et al. Similarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion. PLoS Negl Trop Dis. 2018;12:e0006404 pubmed publisher
	Yuan C, Pu L, He Z, Wang J. BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells. Exp Ther Med. 2018;15:235-241 pubmed publisher
	Doll S, Dreßen M, Geyer P, Itzhak D, Braun C, Doppler S, et al. Region and cell-type resolved quantitative proteomic map of the human heart. Nat Commun. 2017;8:1469 pubmed publisher
	Wang S, Xia P, Chen Y, Qu Y, Xiong Z, Ye B, et al. Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation. Cell. 2017;171:201-216.e18 pubmed publisher
	Subramanian S, Pallati P, Rai V, Sharma P, Agrawal D, Nandipati K. Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance. Obesity (Silver Spring). 2017;25:527-538 pubmed publisher
	Senbabaoglu Y, Gejman R, Winer A, Liu M, Van Allen E, de Velasco G, et al. Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures. Genome Biol. 2016;17:231 pubmed
	Valle Y, Almalki S, Agrawal D. Vitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells. Stem Cell Res Ther. 2016;7:118 pubmed publisher
	Yao Y, Deng Q, Song W, Zhang H, Li Y, Yang Y, et al. MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia. Stem Cell Reports. 2016;7:249-62 pubmed publisher
	Zhu N, Wang H, Wei J, Wang B, Shan W, Lai X, et al. NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells. Mol Med Rep. 2016;14:1351-6 pubmed publisher
	Zhou F, Gao S, Wang L, Sun C, Chen L, Yuan P, et al. Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model. Stem Cell Res Ther. 2015;6:92 pubmed publisher
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	Gao S, Zhao P, Lin C, Sun Y, Wang Y, Zhou Z, et al. Differentiation of human adipose-derived stem cells into neuron-like cells which are compatible with photocurable three-dimensional scaffolds. Tissue Eng Part A. 2014;20:1271-84 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD45 Monoclonal Antibody (2D1), FITC, eBioscience

Catalog # :

11-9459-42

Quantity :

100 Tests

Price :

US 277.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 5 µL (1 µg)/test, Immunohistochemistry (Paraffin): 1:100

Species :

Human

Clone :

2D1

Isotype :

IgG1, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 5 µL (1 µg)/test, Immunohistochemistry (Paraffin): 1:100

Aliases :

B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

more info or order :

Invitrogen product webpage