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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Granzyme B Monoclonal Antibody (NGZB), FITC, eBioscience
catalog :
11-8898-82
quantity :
100 µg
price :
US 361
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
NGZB
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 59
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 1c
Petty A, Dai R, Lapalombella R, Baiocchi R, Benson D, Li Z, et al. Hedgehog-induced PD-L1 on tumor-associated macrophages is critical for suppression of tumor-infiltrating CD8+ T cell function. JCI Insight. 2021;6: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5c
Shi L, Wang J, Ding N, Zhang Y, Zhu Y, Dong S, et al. Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy. Nat Commun. 2019;10:5421 pubmed publisher
  • flow cytometry; mouse; fig 3b
Tavazoie M, Pollack I, Tanqueco R, Ostendorf B, Reis B, Gonsalves F, et al. LXR/ApoE Activation Restricts Innate Immune Suppression in Cancer. Cell. 2018;172:825-840.e18 pubmed publisher
  • flow cytometry; mouse; fig 3b
Cao G, Wang Q, Li G, Meng Z, Liu H, Tong J, et al. mTOR inhibition potentiates cytotoxicity of V?4 ?? T cells via up-regulating NKG2D and TNF-?. J Leukoc Biol. 2016;100:1181-1189 pubmed
  • flow cytometry; mouse; loading ...; fig 5e
Becker P, Hervouet C, Mason G, KWON S, Klavinskis L. Skin vaccination with live virus vectored microneedle arrays induce long lived CD8(+) T cell memory. Vaccine. 2015;33:4691-8 pubmed publisher
Xiao X, Shi J, He C, Bu X, Sun Y, Gao M, et al. ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nat Commun. 2023;14:2859 pubmed publisher
Zhu Z, Tang R, Huff S, Kummetha I, Wang L, Li N, et al. Small-molecule PTPN2 Inhibitors Sensitize Resistant Melanoma to Anti-PD-1 Immunotherapy. Cancer Res Commun. 2023;3:119-129 pubmed publisher
Olivera I, Bola xf1 os E, Gonzalez Gomariz J, Hervas Stubbs S, Mari xf1 o K, Luri Rey C, et al. mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy. Cell Rep Med. 2023;4:100978 pubmed publisher
Liu M, Wang X, Du X, Wu W, Zhang Y, Zhang P, et al. Soluble CTLA-4 mutants ameliorate immune-related adverse events but preserve efficacy of CTLA-4- and PD-1-targeted immunotherapy. Sci Transl Med. 2023;15:eabm5663 pubmed publisher
Ren J, Han X, Lohner H, Hoyle R, Li J, Liang S, et al. P. gingivalis Infection Upregulates PD-L1 Expression on Dendritic Cells, Suppresses CD8+ T-cell Responses, and Aggravates Oral Cancer. Cancer Immunol Res. 2023;11:290-305 pubmed publisher
Li Q, Zhang L, You W, Xu J, Dai J, Hua D, et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun. 2022;13:7677 pubmed publisher
Wang W, Wu S, Cen Z, Zhang Y, Chen Y, Huang Y, et al. Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy. Cell Rep. 2022;41:111582 pubmed publisher
Deng P, Wang Z, Chen J, Liu S, Yao X, Liu S, et al. RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer. J Clin Invest. 2022;132: pubmed publisher
Wong S, McCarroll J, Hsu K, Geczy C, Tedla N. Intranasal Delivery of Recombinant S100A8 Protein Delays Lung Cancer Growth by Remodeling the Lung Immune Microenvironment. Front Immunol. 2022;13:826391 pubmed publisher
Hu B, Yu M, Ma X, Sun J, Liu C, Wang C, et al. IFNα Potentiates Anti-PD-1 Efficacy by Remodeling Glucose Metabolism in the Hepatocellular Carcinoma Microenvironment. Cancer Discov. 2022;12:1718-1741 pubmed publisher
Chen X, Wang K, Jiang S, Sun H, Che X, Zhang M, et al. eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma. J Immunother Cancer. 2022;10: pubmed publisher
Ren J, Li N, Pei S, Lian Y, Li L, Peng Y, et al. Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated anti-tumor immunity. J Clin Invest. 2022;: pubmed publisher
Maity S, Chakraborty A, Mahata S, Roy S, Das A, Sen M. Wnt5A Signaling Blocks Progression of Experimental Visceral Leishmaniasis. Front Immunol. 2022;13:818266 pubmed publisher
Wang Z, Ran L, Chen C, Shi R, Dong Y, Li Y, et al. Identification of HLA-A2-Restricted Mutant Epitopes from Neoantigens of Esophageal Squamous Cell Carcinoma. Vaccines (Basel). 2021;9: pubmed publisher
Li J, Wang S, Wang N, Zheng Y, Yang B, Wang X, et al. Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration. Cell Commun Signal. 2021;19:89 pubmed publisher
Dodagatta Marri E, Ma H, Liang B, Li J, Meyer D, Chen S, et al. Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy. Cell Rep. 2021;36:109309 pubmed publisher
Steele L, Mannion A, Shaw G, MacLennan K, Cook G, Rudd C, et al. Non-redundant activity of GSK-3α and GSK-3β in T cell-mediated tumor rejection. iScience. 2021;24:102555 pubmed publisher
Yang N, Ji F, Cheng L, Lu J, Sun X, Lin X, et al. Knockout of immunotherapy prognostic marker genes eliminates the effect of the anti-PD-1 treatment. NPJ Precis Oncol. 2021;5:37 pubmed publisher
Luo Z, Xia K, Liu Y, Liu J, Rao S, Hu X, et al. Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages. Int J Nanomedicine. 2021;16:2949-2963 pubmed publisher
Daneshmandi S, Cassel T, Lin P, Higashi R, Wulf G, Boussiotis V, et al. Blockade of 6-phosphogluconate dehydrogenase generates CD8+ effector T cells with enhanced anti-tumor function. Cell Rep. 2021;34:108831 pubmed publisher
Liu J, Wang C, Cheng T, Rixiati Y, Ji C, Deng M, et al. Circadian Clock Disruption Suppresses PDL1+ Intraepithelial B Cells in Experimental Colitis and Colitis-Associated Colorectal Cancer. Cell Mol Gastroenterol Hepatol. 2021;12:251-276 pubmed publisher
Ebelt N, Zuniga E, Marzagalli M, Zamloot V, Blazar B, Salgia R, et al. Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy. Biomedicines. 2020;8: pubmed publisher
Utz B, Turpin R, Lampe J, Pouwels J, Klefstrom J. Assessment of the WAP-Myc mouse mammary tumor model for spontaneous metastasis. Sci Rep. 2020;10:18733 pubmed publisher
Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, et al. PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell Dysfunction. Cell Rep. 2020;33:108278 pubmed publisher
Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, et al. Endogenous Glucocorticoid Signaling Regulates CD8+ T Cell Differentiation and Development of Dysfunction in the Tumor Microenvironment. Immunity. 2020;53:658-671.e6 pubmed publisher
Parra M, Weitner M, Yang A, Akue A, Liu X, Schmidt T, et al. Memory CD73+IgM+ B cells protect against Plasmodium yoelii infection and express Granzyme B. PLoS ONE. 2020;15:e0238493 pubmed publisher
Little A, Li Y, Zhang F, Zhang H. Chronic alcohol consumption exacerbates murine cytomegalovirus infection via impairing nonspecific and specific NK activation in mice. FASEB Bioadv. 2019;1:18-31 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Shi B, Wu Y, Wang C, Li X, Yu F, Wang B, et al. Evaluation of antiviral - passive - active immunization ("sandwich") therapeutic strategy for functional cure of chronic hepatitis B in mice. EBioMedicine. 2019;49:247-257 pubmed publisher
Wu J, Li S, Li T, Lv X, Zhang M, Zang G, et al. pDC Activation by TLR7/8 Ligand CL097 Compared to TLR7 Ligand IMQ or TLR9 Ligand CpG. J Immunol Res. 2019;2019:1749803 pubmed publisher
Anderson C, Reilly E, Lee A, Brossay L. Qa-1-Restricted CD8+ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection. Cell Rep. 2019;27:537-548.e5 pubmed publisher
Wang F, Meng M, Mo B, Yang Y, Ji Y, Huang P, et al. Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. Nat Commun. 2018;9:4874 pubmed publisher
Wilgenburg B, Loh L, Chen Z, Pediongco T, Wang H, Shi M, et al. MAIT cells contribute to protection against lethal influenza infection in vivo. Nat Commun. 2018;9:4706 pubmed publisher
Šestan M, Marinović S, Kavazović I, Cekinović Đ, Wueest S, Turk Wensveen T, et al. Virus-Induced Interferon-γ Causes Insulin Resistance in Skeletal Muscle and Derails Glycemic Control in Obesity. Immunity. 2018;49:164-177.e6 pubmed publisher
Smith N, Patel R, Reynaldi A, Grenier J, Wang J, Watson N, et al. Developmental Origin Governs CD8+ T Cell Fate Decisions during Infection. Cell. 2018;174:117-130.e14 pubmed publisher
Kim D, Park H, Lim S, Koo J, Lee H, Choi J, et al. Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis. Nat Commun. 2018;9:503 pubmed publisher
Krzywinska E, Kantari Mimoun C, Kerdiles Y, Sobecki M, Isagawa T, Gotthardt D, et al. Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis. Nat Commun. 2017;8:1597 pubmed publisher
Miao T, Symonds A, Singh R, Symonds J, Ogbe A, Omodho B, et al. Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation. J Exp Med. 2017;214:1787-1808 pubmed publisher
Allen E, Jabouille A, Rivera L, Lodewijckx I, Missiaen R, Steri V, et al. Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation. Sci Transl Med. 2017;9: pubmed publisher
Dai K, Huang L, Huang Y, Chen Z, Yang L, Jiang Y. 1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8+ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model. Front Immunol. 2017;8:320 pubmed publisher
Sonner J, Deumelandt K, Ott M, Thomé C, Rauschenbach K, Schulz S, et al. The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas. Oncoimmunology. 2016;5:e1240858 pubmed publisher
Correia A, Ferreirinha P, Botelho S, Belinha A, Leitao C, Caramalho I, et al. Predominant role of interferon-γ in the host protective effect of CD8(+) T cells against Neospora caninum infection. Sci Rep. 2015;5:14913 pubmed publisher
Teo T, Her Z, Tan J, Lum F, Lee W, Chan Y, et al. Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology. J Virol. 2015;89:7955-69 pubmed publisher
Lunding L, Webering S, Vock C, Behrends J, Wagner C, Hölscher C, et al. Poly(inosinic-cytidylic) acid-triggered exacerbation of experimental asthma depends on IL-17A produced by NK cells. J Immunol. 2015;194:5615-25 pubmed publisher
Li C, Li W, Xiao J, Jiao S, Teng F, Xue S, et al. ADAP and SKAP55 deficiency suppresses PD-1 expression in CD8+ cytotoxic T lymphocytes for enhanced anti-tumor immunotherapy. EMBO Mol Med. 2015;7:754-69 pubmed publisher
Chen L, Gibbons D, Goswami S, Cortez M, Ahn Y, Byers L, et al. Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression. Nat Commun. 2014;5:5241 pubmed publisher
Hou H, Liu W, Wu S, Lu Y, Peng J, Zhu Y, et al. Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock. PLoS ONE. 2014;9:e110585 pubmed publisher
Dai K, Huang L, Chen J, Yang L, Gong Z. Amphiregulin promotes the immunosuppressive activity of intrahepatic CD4+ regulatory T cells to impair CD8+ T-cell immunity against hepatitis B virus infection. Immunology. 2015;144:506-517 pubmed publisher
Brandstadter J, Huang X, Yang Y. NK cell-extrinsic IL-18 signaling is required for efficient NK-cell activation by vaccinia virus. Eur J Immunol. 2014;44:2659-66 pubmed publisher
Kratholm S, Iversen M, Reinert L, Jensen S, Hokland M, Andersen T, et al. Interleukin-21 receptor signalling is important for innate immune protection against HSV-2 infections. PLoS ONE. 2013;8:e81790 pubmed publisher
Fortin C, Huang X, Yang Y. Both NK cell-intrinsic and -extrinsic STAT1 signaling are required for NK cell response against vaccinia virus. J Immunol. 2013;191:363-8 pubmed publisher
Ball E, Sambo M, Martins M, Trovoada M, Benchimol C, Costa J, et al. IFNAR1 controls progression to cerebral malaria in children and CD8+ T cell brain pathology in Plasmodium berghei-infected mice. J Immunol. 2013;190:5118-27 pubmed publisher
Maelfait J, Roose K, Bogaert P, Sze M, Saelens X, Pasparakis M, et al. A20 (Tnfaip3) deficiency in myeloid cells protects against influenza A virus infection. PLoS Pathog. 2012;8:e1002570 pubmed publisher
Smyth M, Trapani J. Granzymes: exogenous proteinases that induce target cell apoptosis. Immunol Today. 1995;16:202-6 pubmed
product information
Product Type :
Antibody
Product Name :
Granzyme B Monoclonal Antibody (NGZB), FITC, eBioscience
Catalog # :
11-8898-82
Quantity :
100 µg
Price :
US 361
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.125 µg/test
Species :
Mouse
Clone :
NGZB
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.125 µg/test
Aliases :
AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA