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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IL-6 Monoclonal Antibody (MP5-20F3), FITC, eBioscience™
catalog :
11-7061-82
quantity :
100 µg
price :
US 309.00
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
MP5-20F3
reactivity :
mouse
application :
flow cytometry
more info or order :
Invitrogen product webpage
citations: 69
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 4A
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Macia A, Vaquero M, Gou Fabregas M, Castelblanco E, Valdivielso J, Anerillas C, et al. Sprouty1 induces a senescence-associated secretory phenotype by regulating NF?B activity: implications for tumorigenesis. Cell Death Differ. 2014;21:333-43 pubmed publisher
Yokota Nakatsuma A, Takeuchi H, Ohoka Y, Kato C, Song S, Hoshino T, et al. Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells. Mucosal Immunol. 2014;7:786-801 pubmed publisher
Konry T, Golberg A, Yarmush M. Live single cell functional phenotyping in droplet nano-liter reactors. Sci Rep. 2013;3:3179 pubmed publisher
Arocena A, Onofrio L, Pellegrini A, Carrera Silva A, Paroli A, Cano R, et al. Myeloid-derived suppressor cells are key players in the resolution of inflammation during a model of acute infection. Eur J Immunol. 2014;44:184-94 pubmed publisher
Steinbach K, Piedavent M, Bauer S, Neumann J, Friese M. Neutrophils amplify autoimmune central nervous system infiltrates by maturing local APCs. J Immunol. 2013;191:4531-9 pubmed publisher
Kawashima H, Takatori H, Suzuki K, Iwata A, Yokota M, Suto A, et al. Tumor suppressor p53 inhibits systemic autoimmune diseases by inducing regulatory T cells. J Immunol. 2013;191:3614-23 pubmed publisher
Fermin Lee A, Chen H, Wan L, Wu S, Yu J, Huang A, et al. Galectin-3 modulates Th17 responses by regulating dendritic cell cytokines. Am J Pathol. 2013;183:1209-1222 pubmed publisher
Maldonado M, Kapphahn R, Terluk M, Heuss N, Yuan C, Gregerson D, et al. Immunoproteasome deficiency modifies the alternative pathway of NF?B signaling. PLoS ONE. 2013;8:e56187 pubmed publisher
Lee E, Park C, Jung Y. Anti-inflammatory and immune-modulating effect of Ulmus davidiana var. japonica Nakai extract on a macrophage cell line and immune cells in the mouse small intestine. J Ethnopharmacol. 2013;146:608-13 pubmed publisher
Yee N, Hamerman J. ?(2) integrins inhibit TLR responses by regulating NF-?B pathway and p38 MAPK activation. Eur J Immunol. 2013;43:779-92 pubmed publisher
Park S, Kang S, Min K, Woo Hwang K, Min H. Age-associated changes in microRNA expression in bone marrow derived dendritic cells. Immunol Invest. 2013;42:179-90 pubmed publisher
Hoge J, Yan I, Jänner N, Schumacher V, Chalaris A, Steinmetz O, et al. IL-6 controls the innate immune response against Listeria monocytogenes via classical IL-6 signaling. J Immunol. 2013;190:703-11 pubmed publisher
Chu Y, Soberon V, Glockner L, Beyaert R, Massoumi R, Van Loo G, et al. A20 and CYLD do not share significant overlapping functions during B cell development and activation. J Immunol. 2012;189:4437-43 pubmed publisher
Xu Y, Fan H, Li X, Sun L, Hou Y. 17?-Estradiol enhances response of mice spleen B cells elicited by TLR9 agonist. Cell Immunol. 2012;278:125-35 pubmed publisher
Cai G, Nie X, Zhang W, Wu B, Lin J, Wang H, et al. A regulatory role for IL-10 receptor signaling in development and B cell help of T follicular helper cells in mice. J Immunol. 2012;189:1294-302 pubmed publisher
Glowacka W, Alberts P, Ouchida R, Wang J, Rotin D. LAPTM5 protein is a positive regulator of proinflammatory signaling pathways in macrophages. J Biol Chem. 2012;287:27691-702 pubmed publisher
Buchwald Z, Kiesel J, DiPaolo R, Pagadala M, Aurora R. Osteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption in vitro. PLoS ONE. 2012;7:e38199 pubmed publisher
Osterbur J, Sprague L, Muccioli M, Pate M, Mansfield K, McGinty J, et al. Adhesion to substrates induces dendritic cell endothelization and decreases immunological response. Immunobiology. 2013;218:64-75 pubmed publisher
Heib V, Sparber F, Tripp C, Ortner D, Stoitzner P, Heufler C. Cytip regulates dendritic-cell function in contact hypersensitivity. Eur J Immunol. 2012;42:589-97 pubmed publisher
Ramon H, Beal A, Liu Y, Worthen G, Oliver P. The E3 ubiquitin ligase adaptor Ndfip1 regulates Th17 differentiation by limiting the production of proinflammatory cytokines. J Immunol. 2012;188:4023-31 pubmed publisher
Iguchi Hashimoto M, Usui T, Yoshifuji H, Shimizu M, Kobayashi S, Ito Y, et al. Overexpression of a minimal domain of calpastatin suppresses IL-6 production and Th17 development via reduced NF-?B and increased STAT5 signals. PLoS ONE. 2011;6:e27020 pubmed publisher
Massilamany C, Gangaplara A, Steffen D, Reddy J. Identification of novel mimicry epitopes for cardiac myosin heavy chain-? that induce autoimmune myocarditis in A/J mice. Cell Immunol. 2011;271:438-49 pubmed publisher
Elliott E, Van Ziffle J, Scapini P, Sullivan B, Locksley R, Lowell C. Deletion of Syk in neutrophils prevents immune complex arthritis. J Immunol. 2011;187:4319-30 pubmed publisher
O Dea K, Dokpesi J, Tatham K, Wilson M, Takata M. Regulation of monocyte subset proinflammatory responses within the lung microvasculature by the p38 MAPK/MK2 pathway. Am J Physiol Lung Cell Mol Physiol. 2011;301:L812-21 pubmed publisher
Hemmers S, Teijaro J, Arandjelovic S, Mowen K. PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection. PLoS ONE. 2011;6:e22043 pubmed publisher
Sprague L, Muccioli M, Pate M, Meles E, McGinty J, Nandigam H, et al. The interplay between surfaces and soluble factors define the immunologic and angiogenic properties of myeloid dendritic cells. BMC Immunol. 2011;12:35 pubmed publisher
Groppo R, Richter J. CPEB control of NF-kappaB nuclear localization and interleukin-6 production mediates cellular senescence. Mol Cell Biol. 2011;31:2707-14 pubmed publisher
Diao J, Zhao J, Winter E, Cattral M. Tumors suppress in situ proliferation of cytotoxic T cells by promoting differentiation of Gr-1(+) conventional dendritic cells through IL-6. J Immunol. 2011;186:5058-67 pubmed publisher
Molinero L, Miller M, Evaristo C, Alegre M. High TCR stimuli prevent induced regulatory T cell differentiation in a NF-?B-dependent manner. J Immunol. 2011;186:4609-17 pubmed publisher
Ashman R, Goeken J, Latz E, Lenert P. Optimal oligonucleotide sequences for TLR9 inhibitory activity in human cells: lack of correlation with TLR9 binding. Int Immunol. 2011;23:203-14 pubmed publisher
Helmersson S, Stenstrom M, Leanderson T, Ivars F. Specific effect of immunomodulatory quinoline-3-carboxamide ABR-215757 in GM-CSF stimulated bone marrow cell cultures: block of initiation of proliferation of Gr-1+ cells. Int Immunopharmacol. 2011;11:1045-51 pubmed publisher
Ashman R, Goeken J, Lenert P. Aggregation and secondary loop structure of oligonucleotides do not determine their ability to inhibit TLR9. Int Immunopharmacol. 2011;11:1032-7 pubmed publisher
Bao Y, Han Y, Chen Z, Xu S, Cao X. IFN-?-producing PDCA-1+ Siglec-H- B cells mediate innate immune defense by activating NK cells. Eur J Immunol. 2011;41:657-68 pubmed publisher
Layer T, Steele A, Goeken J, Fleenor S, Lenert P. Engagement of the B cell receptor for antigen differentially affects B cell responses to Toll-like receptor-7 agonists and antagonists in BXSB mice. Clin Exp Immunol. 2011;163:392-403 pubmed publisher
Joetham A, Okamoto M, Takeda K, Schedel M, Ohnishi H, Dakhama A, et al. CD8 regulates T regulatory cell production of IL-6 and maintains their suppressive phenotype in allergic lung disease. J Immunol. 2011;186:113-20 pubmed publisher
Fan J, Edsen Moore M, Turner L, Cook R, Legge K, Waldschmidt T, et al. Mechanisms by which chronic ethanol feeding limits the ability of dendritic cells to stimulate T-cell proliferation. Alcohol Clin Exp Res. 2011;35:47-59 pubmed publisher
Hwang W, Jung K, Jeon Y, Yun S, Kim T, Choi I. Knockdown of the interleukin-6 receptor alpha chain of dendritic cell vaccines enhances the therapeutic potential against IL-6 producing tumors. Vaccine. 2010;29:34-44 pubmed publisher
Goeken J, Layer T, Fleenor S, Laccheo M, Lenert P. B-cell receptor for antigen modulates B-cell responses to complex TLR9 agonists and antagonists: implications for systemic lupus erythematosus. Lupus. 2010;19:1290-301 pubmed publisher
Piconese S, Gri G, Tripodo C, Musio S, Gorzanelli A, Frossi B, et al. Mast cells counteract regulatory T-cell suppression through interleukin-6 and OX40/OX40L axis toward Th17-cell differentiation. Blood. 2009;114:2639-48 pubmed publisher
Guarda G, Dostert C, Staehli F, Cabalzar K, Castillo R, Tardivel A, et al. T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes. Nature. 2009;460:269-73 pubmed publisher
Fang C, Zhang X, Miwa T, Song W. Complement promotes the development of inflammatory T-helper 17 cells through synergistic interaction with Toll-like receptor signaling and interleukin-6 production. Blood. 2009;114:1005-15 pubmed publisher
Wu Y, El Shikh M, El Sayed R, Best A, Szakal A, Tew J. IL-6 produced by immune complex-activated follicular dendritic cells promotes germinal center reactions, IgG responses and somatic hypermutation. Int Immunol. 2009;21:745-56 pubmed publisher
Wu C, Rauch U, Korpos E, Song J, Loser K, Crocker P, et al. Sialoadhesin-positive macrophages bind regulatory T cells, negatively controlling their expansion and autoimmune disease progression. J Immunol. 2009;182:6508-16 pubmed publisher
Yamashita K, Sakai M, Takemoto N, Tsukimoto M, Uchida K, Yajima H, et al. Attenuation of delayed-type hypersensitivity by fullerene treatment. Toxicology. 2009;261:19-24 pubmed publisher
Maynard C, Hatton R, Helms W, Oliver J, Stephensen C, Weaver C. Contrasting roles for all-trans retinoic acid in TGF-beta-mediated induction of Foxp3 and Il10 genes in developing regulatory T cells. J Exp Med. 2009;206:343-57 pubmed publisher
Tsukimoto M, Nakatsukasa H, Sugawara K, Yamashita K, Kojima S. Repeated 0.5-Gy gamma irradiation attenuates experimental autoimmune encephalomyelitis with up-regulation of regulatory T cells and suppression of IL17 production. Radiat Res. 2008;170:429-36 pubmed
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Rankin A, Reed A, Oh S, Cozzo Picca C, Guay H, Larkin J, et al. CD4+ T cells recognizing a single self-peptide expressed by APCs induce spontaneous autoimmune arthritis. J Immunol. 2008;180:833-41 pubmed
Tago F, Tsukimoto M, Nakatsukasa H, Kojima S. Repeated 0.5-Gy gamma irradiation attenuates autoimmune disease in MRL-lpr/lpr mice with suppression of CD3+CD4-CD8-B220+ T-cell proliferation and with up-regulation of CD4+CD25+Foxp3+ regulatory T cells. Radiat Res. 2008;169:59-66 pubmed
Pejcic Karapetrovic B, Gurnani K, Russell M, Finlay B, Sad S, Krishnan L. Pregnancy impairs the innate immune resistance to Salmonella typhimurium leading to rapid fatal infection. J Immunol. 2007;179:6088-96 pubmed
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product information
Product Type :
Antibody
Product Name :
IL-6 Monoclonal Antibody (MP5-20F3), FITC, eBioscience™
Catalog # :
11-7061-82
Quantity :
100 µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
MP5-20F3
Isotype :
IgG1, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA