IgM Monoclonal Antibody (eB121-15F9), FITC, eBioscience™ | Invitrogen 11-5890-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

IgM Monoclonal Antibody (eB121-15F9), FITC, eBioscience™

catalog :

11-5890-82

quantity :

100 µg

price :

US 309.00

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

eB121-15F9

reactivity :

mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	Mercadante A, Perobelli S, Alves A, Gonçalves Silva T, Mello W, Gomes Santos A, et al. Oral combined therapy with probiotics and alloantigen induces B cell-dependent long-lasting specific tolerance. J Immunol. 2014;192:1928-37 pubmed publisher
	Gal Oz S, Maier B, Yoshida H, Seddu K, Elbaz N, Czysz C, et al. ImmGen report: sexual dimorphism in the immune system transcriptome. Nat Commun. 2019;10:4295 pubmed publisher
	Soukup A, Zheng Y, Mehta C, Wu J, Liu P, Cao M, et al. Single-nucleotide human disease mutation inactivates a blood-regenerative GATA2 enhancer. J Clin Invest. 2019;129:1180-1192 pubmed publisher
	Katsumura K, Mehta C, Hewitt K, Soukup A, Fraga de Andrade I, Ranheim E, et al. Human leukemia mutations corrupt but do not abrogate GATA-2 function. Proc Natl Acad Sci U S A. 2018;115:E10109-E10118 pubmed publisher
	Nowsheen S, Aziz K, Luo K, Deng M, Qin B, Yuan J, et al. ZNF506-dependent positive feedback loop regulates H2AX signaling after DNA damage. Nat Commun. 2018;9:2736 pubmed publisher
	Ribeiro de Almeida C, Dhir S, Dhir A, Moghaddam A, Sattentau Q, Meinhart A, et al. RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination. Mol Cell. 2018;70:650-662.e8 pubmed publisher
	El Zaatari M, Bass A, Bowlby R, Zhang M, Syu L, Yang Y, et al. Indoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity. Gastroenterology. 2018;154:140-153.e17 pubmed publisher
	Le Noir S, Laffleur B, Carrion C, Garot A, Lecardeur S, Pinaud E, et al. The IgH locus 3' cis-regulatory super-enhancer co-opts AID for allelic transvection. Oncotarget. 2017;8:12929-12940 pubmed publisher
	Nguyen T, Pawlikowska P, Firlej V, Rosselli F, Aoufouchi S. V(D)J recombination process and the Pre-B to immature B-cells transition are altered in Fanca-/- mice. Sci Rep. 2016;6:36906 pubmed publisher
	Alrefai H, Muhammad K, Rudolf R, Pham D, Klein Hessling S, Patra A, et al. NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells. Nat Commun. 2016;7:11724 pubmed publisher
	Johnson K, Kong G, Gao X, Chang Y, Hewitt K, Sanalkumar R, et al. Cis-regulatory mechanisms governing stem and progenitor cell transitions. Sci Adv. 2015;1:e1500503 pubmed publisher
	Zhang S, Zhang H, Yang T, Ying T, Yang P, Liu X, et al. Interaction between HIV-1 Tat and DNA-PKcs modulates HIV transcription and class switch recombination. Int J Biol Sci. 2014;10:1138-49 pubmed publisher
	Hanihara Tatsuzawa F, Miura H, Kobayashi S, Isagawa T, Okuma A, Manabe I, et al. Control of Toll-like receptor-mediated T cell-independent type 1 antibody responses by the inducible nuclear protein I?B-?. J Biol Chem. 2014;289:30925-36 pubmed publisher
	Ding Z, Bergman A, Rutemark C, Ouchida R, Ohno H, Wang J, et al. Complement-activating IgM enhances the humoral but not the T cell immune response in mice. PLoS ONE. 2013;8:e81299 pubmed publisher
	Donius L, Handy J, Weis J, Weis J. Optimal germinal center B cell activation and T-dependent antibody responses require expression of the mouse complement receptor Cr1. J Immunol. 2013;191:434-47 pubmed publisher
	Pei H, Wu X, Liu T, Yu K, Jelinek D, Lou Z. The histone methyltransferase MMSET regulates class switch recombination. J Immunol. 2013;190:756-63 pubmed publisher
	Iseki M, Omori Miyake M, Xu W, Sun X, Takaki S, Rawlings D, et al. Thymic stromal lymphopoietin (TSLP)-induced polyclonal B-cell activation and autoimmunity are mediated by CD4+ T cells and IL-4. Int Immunol. 2012;24:183-95 pubmed publisher
	Sawaguchi M, Tanaka S, Nakatani Y, Harada Y, Mukai K, Matsunaga Y, et al. Role of mast cells and basophils in IgE responses and in allergic airway hyperresponsiveness. J Immunol. 2012;188:1809-18 pubmed publisher
	Allman W, Saini S, Tuzun E, Christadoss P. Characterization of peripheral blood acetylcholine receptor-binding B cells in experimental myasthenia gravis. Cell Immunol. 2011;271:292-8 pubmed publisher
	Wang J, Lee J, Yan M, Rho J, Roehrl M. Dermatan sulfate interacts with dead cells and regulates CD5(+) B-cell fate: implications for a key role in autoimmunity. Am J Pathol. 2011;178:2168-76 pubmed publisher
	Shiokawa A, Tanabe K, Tsuji N, Sato R, Hachimura S. IL-10 and IL-27 producing dendritic cells capable of enhancing IL-10 production of T cells are induced in oral tolerance. Immunol Lett. 2009;125:7-14 pubmed publisher
	Benatar T, Cao M, Lee Y, Li H, Feng N, Gu X, et al. Virulizin induces production of IL-17E to enhance antitumor activity by recruitment of eosinophils into tumors. Cancer Immunol Immunother. 2008;57:1757-69 pubmed publisher
	Chen X, Esplin B, Garrett K, Welner R, Webb C, Kincade P. Retinoids accelerate B lineage lymphoid differentiation. J Immunol. 2008;180:138-45 pubmed
	Onai N, Obata Onai A, Schmid M, Ohteki T, Jarrossay D, Manz M. Identification of clonogenic common Flt3+M-CSFR+ plasmacytoid and conventional dendritic cell progenitors in mouse bone marrow. Nat Immunol. 2007;8:1207-16 pubmed
	Burchill M, Yang J, Vogtenhuber C, Blazar B, Farrar M. IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells. J Immunol. 2007;178:280-90 pubmed
	Nojima T, Hayashi K, Goitsuka R, Nakayama K, Nakayama K, Kitamura D. Double knockout mice show BASH and PKCdelta have different epistatic relationships in B cell maturation and CD40-mediated activation. Immunol Lett. 2006;105:48-54 pubmed

product information

Product Type :

Antibody

Product Name :

IgM Monoclonal Antibody (eB121-15F9), FITC, eBioscience™

Catalog # :

11-5890-82

Quantity :

100 µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.5 µg/test

Species :

Mouse

Clone :

eB121-15F9

Isotype :

IgG2a, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test

Aliases :

AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH

more info or order :

Invitrogen product webpage