product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45.2 Monoclonal Antibody (104), FITC, eBioscience™
catalog :
11-0454-81
quantity :
50 µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
104
reactivity :
mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, other
more info or order :
citations: 107
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s6a
Hall B, Gleiberman A, Strom E, Krasnov P, Frescas D, Vujcic S, et al. Immune checkpoint protein VSIG4 as a biomarker of aging in murine adipose tissue. Aging Cell. 2020;19:e13219 pubmed publisher
  • flow cytometry; mouse; 1:500; fig 6g
Gang E, Kim H, Hsieh Y, Ruan Y, Ogana H, Lee S, et al. Integrin α6 mediates the drug resistance of acute lymphoblastic B-cell leukemia. Blood. 2020;136:210-223 pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig 2f, 2g
Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s4b
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • flow cytometry; mouse; 1:100; tbl 1
  • immunocytochemistry; mouse; 1:100; tbl 1
Jackson Jones L, Duncan S, Magalhaes M, Campbell S, Maizels R, McSorley H, et al. Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nat Commun. 2016;7:12651 pubmed publisher
  • immunohistochemistry - frozen section; mouse
  • flow cytometry; mouse
Jacque E, Schweighoffer E, Visekruna A, Papoutsopoulou S, Janzen J, Zillwood R, et al. IKK-induced NF-κB1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen. J Exp Med. 2014;211:2085-101 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Cowan J, McCarthy N, Parnell S, White A, Bacon A, Serge A, et al. Differential requirement for CCR4 and CCR7 during the development of innate and adaptive ??T cells in the adult thymus. J Immunol. 2014;193:1204-12 pubmed publisher
  • other; mouse; fig 1
Barral P, Sanchez Nino M, Van Rooijen N, Cerundolo V, Batista F. The location of splenic NKT cells favours their rapid activation by blood-borne antigen. EMBO J. 2012;31:2378-90 pubmed publisher
  • flow cytometry; mouse; fig 3
Suliman S, Tan J, Xu K, Kousis P, Kowalski P, Chang G, et al. Notch3 is dispensable for thymocyte ?-selection and Notch1-induced T cell leukemogenesis. PLoS ONE. 2011;6:e24937 pubmed publisher
  • flow cytometry; mouse
Schaeffer M, Han S, Chtanova T, van Dooren G, Herzmark P, Chen Y, et al. Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii. J Immunol. 2009;182:6379-93 pubmed publisher
  • flow cytometry; mouse
Taylor R, Patel S, Lin E, Butler B, Lake J, Newberry R, et al. Lymphotoxin-independent expression of TNF-related activation-induced cytokine by stromal cells in cryptopatches, isolated lymphoid follicles, and Peyer's patches. J Immunol. 2007;178:5659-67 pubmed
Chen C, Yu W, Tober J, Gao P, He B, Lee K, et al. Spatial Genome Re-organization between Fetal and Adult Hematopoietic Stem Cells. Cell Rep. 2019;29:4200-4211.e7 pubmed publisher
Trevejo Nuñez G, Elsegeiny W, Aggor F, Tweedle J, Kaplan Z, Gandhi P, et al. Interleukin-22 (IL-22) Binding Protein Constrains IL-22 Activity, Host Defense, and Oxidative Phosphorylation Genes during Pneumococcal Pneumonia. Infect Immun. 2019;87: pubmed publisher
Contreras O, Cruz Soca M, Theret M, Soliman H, Tung L, Groppa E, et al. Cross-talk between TGF-β and PDGFRα signaling pathways regulates the fate of stromal fibro-adipogenic progenitors. J Cell Sci. 2019;132: pubmed publisher
Yamashita M, Passegue E. TNF-α Coordinates Hematopoietic Stem Cell Survival and Myeloid Regeneration. Cell Stem Cell. 2019;: pubmed publisher
Janela B, Patel A, Lau M, Goh C, Msallam R, Kong W, et al. A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils. Immunity. 2019;50:1069-1083.e8 pubmed publisher
Zhao M, Tao F, Venkatraman A, Li Z, Smith S, Unruh J, et al. N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells. Cell Rep. 2019;26:652-669.e6 pubmed publisher
Knolle M, Chin S, Rana B, Englezakis A, Nakagawa R, Fallon P, et al. MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity. Front Immunol. 2018;9:2232 pubmed publisher
Moretti F, Klapproth S, Ruppert R, Margraf A, Weber J, Pick R, et al. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus. elife. 2018;7: pubmed publisher
Wang H, Hogquist K. CCR7 defines a precursor for murine iNKT cells in thymus and periphery. elife. 2018;7: pubmed publisher
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Qian P, De Kumar B, He X, Nolte C, Gogol M, Ahn Y, et al. Retinoid-Sensitive Epigenetic Regulation of the Hoxb Cluster Maintains Normal Hematopoiesis and Inhibits Leukemogenesis. Cell Stem Cell. 2018;22:740-754.e7 pubmed publisher
Kramann R, Machado F, Wu H, Kusaba T, Hoeft K, Schneider R, et al. Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis. JCI Insight. 2018;3: pubmed publisher
Li H, Yao Q, Mariscal A, Wu X, Hulse J, Pedersen E, et al. Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation. Nat Commun. 2018;9:1420 pubmed publisher
Ohno Urabe S, Aoki H, Nishihara M, Furusho A, Hirakata S, Nishida N, et al. Role of Macrophage Socs3 in the Pathogenesis of Aortic Dissection. J Am Heart Assoc. 2018;7: pubmed publisher
Guo X, Chu L, Ke F, Mu L, Li Z, Cai J, et al. Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells. Oncotarget. 2017;8:108981-108988 pubmed publisher
Wang Q, He H, Chen D, Wang C, Xu Y, Song W. Hepatic stroma-educated regulatory DCs suppress CD8+ T cell proliferation in mice. Oncotarget. 2017;8:93414-93425 pubmed publisher
Kim S, Kim H, Yim Y, Ha S, Atarashi K, Tan T, et al. Maternal gut bacteria promote neurodevelopmental abnormalities in mouse offspring. Nature. 2017;549:528-532 pubmed publisher
Zhu Y, Herndon J, Sojka D, Kim K, Knolhoff B, Zuo C, et al. Tissue-Resident Macrophages in Pancreatic Ductal Adenocarcinoma Originate from Embryonic Hematopoiesis and Promote Tumor Progression. Immunity. 2017;47:323-338.e6 pubmed publisher
Ni G, Liao Z, Chen S, Wang T, Yuan J, Pan X, et al. Blocking IL-10 signalling at the time of immunization does not increase unwanted side effects in mice. BMC Immunol. 2017;18:40 pubmed publisher
Hayatsu N, Miyao T, Tachibana M, Murakami R, Kimura A, Kato T, et al. Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells. Immunity. 2017;47:268-283.e9 pubmed publisher
Canfrán Duque A, Rotllan N, Zhang X, Fernández Fuertes M, Ramírez Hidalgo C, Araldi E, et al. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis. EMBO Mol Med. 2017;9:1244-1262 pubmed publisher
Miao T, Symonds A, Singh R, Symonds J, Ogbe A, Omodho B, et al. Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation. J Exp Med. 2017;214:1787-1808 pubmed publisher
Zhao Y, Wu K, Nguyen C, Smbatyan G, Melendez E, Higuchi Y, et al. Small molecule p300/catenin antagonist enhances hematopoietic recovery after radiation. PLoS ONE. 2017;12:e0177245 pubmed publisher
King A, Houlihan D, Kavanagh D, Haldar D, Luu N, Owen A, et al. Sphingosine-1-Phosphate Prevents Egress of Hematopoietic Stem Cells From Liver to Reduce Fibrosis. Gastroenterology. 2017;153:233-248.e16 pubmed publisher
Eddens T, Elsegeiny W, Garcia Hernadez M, Castillo P, Trevejo Nuñez G, Serody K, et al. Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity. Cell Rep. 2017;18:3078-3090 pubmed publisher
Hérault A, Binnewies M, Leong S, Calero Nieto F, Zhang S, Kang Y, et al. Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis. Nature. 2017;544:53-58 pubmed publisher
Ho T, Warr M, Adelman E, Lansinger O, Flach J, Verovskaya E, et al. Autophagy maintains the metabolism and function of young and old stem cells. Nature. 2017;543:205-210 pubmed publisher
Asada N, Kunisaki Y, Pierce H, Wang Z, Fernandez N, Birbrair A, et al. Differential cytokine contributions of perivascular haematopoietic stem cell niches. Nat Cell Biol. 2017;19:214-223 pubmed publisher
Zamarin D, Holmgaard R, Ricca J, Plitt T, Palese P, Sharma P, et al. Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity. Nat Commun. 2017;8:14340 pubmed publisher
Herrtwich L, Nanda I, Evangelou K, Nikolova T, Horn V, Sagar -, et al. DNA Damage Signaling Instructs Polyploid Macrophage Fate in Granulomas. Cell. 2016;167:1264-1280.e18 pubmed publisher
Hahm E, Wei C, Fernandez I, Li J, Tardi N, Tracy M, et al. Bone marrow-derived immature myeloid cells are a main source of circulating suPAR contributing to proteinuric kidney disease. Nat Med. 2017;23:100-106 pubmed publisher
Chong S, Evrard M, Devi S, Chen J, Lim J, See P, et al. CXCR4 identifies transitional bone marrow premonocytes that replenish the mature monocyte pool for peripheral responses. J Exp Med. 2016;213:2293-2314 pubmed
Chudnovskiy A, Mortha A, Kana V, Kennard A, Ramírez J, Rahman A, et al. Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome. Cell. 2016;167:444-456.e14 pubmed publisher
Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
Shi Y, Wu W, Chai Q, Li Q, Hou Y, Xia H, et al. LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration. Nat Commun. 2016;7:12369 pubmed publisher
Mucci A, Kunkiel J, Suzuki T, Brennig S, Glage S, Kühnel M, et al. Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency. Stem Cell Reports. 2016;7:292-305 pubmed publisher
Barrett N, Malouf C, Kapeni C, Bacon W, Giotopoulos G, Jacobsen S, et al. Mll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during a Restricted Window in Development. Cell Rep. 2016;16:1039-1054 pubmed publisher
Pietras E, Mirantes Barbeito C, Fong S, Loeffler D, Kovtonyuk L, Zhang S, et al. Chronic interleukin-1 exposure drives haematopoietic stem cells towards precocious myeloid differentiation at the expense of self-renewal. Nat Cell Biol. 2016;18:607-18 pubmed publisher
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Tian D, Liang Y, Zhang Y. Endothelium-targeted human Delta-like 1 enhances the regeneration and homing of human cord blood stem and progenitor cells. J Transl Med. 2016;14:5 pubmed publisher
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Jacque E, Schweighoffer E, Tybulewicz V, Ley S. BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival. J Exp Med. 2015;212:883-92 pubmed publisher
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Song J, Wu C, Korpos E, Zhang X, Agrawal S, Wang Y, et al. Focal MMP-2 and MMP-9 activity at the blood-brain barrier promotes chemokine-induced leukocyte migration. Cell Rep. 2015;10:1040-54 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD45.2 Monoclonal Antibody (104), FITC, eBioscience™
Catalog # :
11-0454-81
Quantity :
50 µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
104
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA