SIGLEC H Monoclonal Antibody (eBio440c), FITC, eBioscience™ | Invitrogen 11-0333-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SIGLEC H Monoclonal Antibody (eBio440c), FITC, eBioscience™

catalog :

11-0333-82

quantity :

100 µg

price :

US 309.00

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

eBio440c

reactivity :

mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 1d	Panda S, Facchinetti V, Voynova E, Hanabuchi S, Karnell J, Hanna R, et al. Galectin-9 inhibits TLR7-mediated autoimmunity in murine lupus models. J Clin Invest. 2018;128:1873-1887 pubmed publisher
flow cytometry; mouse; fig 6b	Wu X, Satpathy A, Kc W, Liu P, Murphy T, Murphy K. Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo. PLoS ONE. 2013;8:e64800 pubmed publisher
flow cytometry; mouse; fig 7	Charles E, Joshi S, Ash J, Fox B, Farris A, Bzik D, et al. CD4 T-cell suppression by cells from Toxoplasma gondii-infected retinas is mediated by surface protein PD-L1. Infect Immun. 2010;78:3484-92 pubmed publisher
	Lehmann C, Baranska A, Heidkamp G, Heger L, Neubert K, Lühr J, et al. DC subset-specific induction of T cell responses upon antigen uptake via Fc? receptors in vivo. J Exp Med. 2017;214:1509-1528 pubmed publisher
	Everaere L, Ait Yahia S, Molendi Coste O, Vorng H, Quemener S, LeVu P, et al. Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity. J Allergy Clin Immunol. 2016;138:1309-1318.e11 pubmed publisher
	Riepsaame J, van Oudenaren A, den Broeder B, van Ijcken W, Pothof J, Leenen P. MicroRNA-Mediated Down-Regulation of M-CSF Receptor Contributes to Maturation of Mouse Monocyte-Derived Dendritic Cells. Front Immunol. 2013;4:353 pubmed publisher
	Liu H, Ramachandran I, Gabrilovich D. Regulation of plasmacytoid dendritic cell development in mice by aryl hydrocarbon receptor. Immunol Cell Biol. 2014;92:200-3 pubmed publisher
	Maina V, Marrella V, Mantero S, Cassani B, Fontana E, Anselmo A, et al. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration. J Leukoc Biol. 2013;94:1221-30 pubmed publisher
	Baccarella A, Fontana M, Chen E, Kim C. Toll-like receptor 7 mediates early innate immune responses to malaria. Infect Immun. 2013;81:4431-42 pubmed publisher
	Oderup C, Lajevic M, Butcher E. Canonical and noncanonical Wnt proteins program dendritic cell responses for tolerance. J Immunol. 2013;190:6126-34 pubmed publisher
	Zeng R, Oderup C, Yuan R, Lee M, Habtezion A, Hadeiba H, et al. Retinoic acid regulates the development of a gut-homing precursor for intestinal dendritic cells. Mucosal Immunol. 2013;6:847-56 pubmed publisher
	Kim C, Nelson C, Wilson E, Hou B, DeFranco A, Derisi J. Splenic red pulp macrophages produce type I interferons as early sentinels of malaria infection but are dispensable for control. PLoS ONE. 2012;7:e48126 pubmed publisher
	Crother T, Ma J, Jupelli M, Chiba N, Chen S, Slepenkin A, et al. Plasmacytoid dendritic cells play a role for effective innate immune responses during Chlamydia pneumoniae infection in mice. PLoS ONE. 2012;7:e48655 pubmed publisher
	Lombardi V, Speak A, Kerzerho J, Szely N, Akbari O. CD8???? and CD8???? plasmacytoid dendritic cells induce Foxp3? regulatory T cells and prevent the induction of airway hyper-reactivity. Mucosal Immunol. 2012;5:432-43 pubmed publisher
	Hadeiba H, Lahl K, Edalati A, Oderup C, Habtezion A, Pachynski R, et al. Plasmacytoid dendritic cells transport peripheral antigens to the thymus to promote central tolerance. Immunity. 2012;36:438-50 pubmed publisher
	Ho A, Prabhu N, Betts R, Ge M, Dai X, Hutchinson P, et al. Lung CD103+ dendritic cells efficiently transport influenza virus to the lymph node and load viral antigen onto MHC class I for presentation to CD8 T cells. J Immunol. 2011;187:6011-21 pubmed publisher
	Davidson S, Kaiko G, Loh Z, Lalwani A, Zhang V, Spann K, et al. Plasmacytoid dendritic cells promote host defense against acute pneumovirus infection via the TLR7-MyD88-dependent signaling pathway. J Immunol. 2011;186:5938-48 pubmed publisher
	Bao Y, Han Y, Chen Z, Xu S, Cao X. IFN-?-producing PDCA-1+ Siglec-H- B cells mediate innate immune defense by activating NK cells. Eur J Immunol. 2011;41:657-68 pubmed publisher
	Gregorio J, Meller S, Conrad C, Di Nardo A, Homey B, Lauerma A, et al. Plasmacytoid dendritic cells sense skin injury and promote wound healing through type I interferons. J Exp Med. 2010;207:2921-30 pubmed publisher
	Hermesh T, Moltedo B, Moran T, LOPEZ C. Antiviral instruction of bone marrow leukocytes during respiratory viral infections. Cell Host Microbe. 2010;7:343-53 pubmed publisher
	Provoost S, Maes T, Willart M, Joos G, Lambrecht B, Tournoy K. Diesel exhaust particles stimulate adaptive immunity by acting on pulmonary dendritic cells. J Immunol. 2010;184:426-32 pubmed publisher
	Blasius A, Giurisato E, Cella M, Schreiber R, Shaw A, Colonna M. Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation. J Immunol. 2006;177:3260-5 pubmed
	Blasius A, Cella M, Maldonado J, Takai T, Colonna M. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood. 2006;107:2474-6 pubmed
	Blasius A, Vermi W, Krug A, Facchetti F, Cella M, Colonna M. A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood. 2004;103:4201-6 pubmed

product information

Product Type :

Antibody

Product Name :

SIGLEC H Monoclonal Antibody (eBio440c), FITC, eBioscience™

Catalog # :

11-0333-82

Quantity :

100 µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.25 µg/test

Species :

Mouse

Clone :

eBio440c

Isotype :

IgG2b, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.25 µg/test

Aliases :

6430529G09Rik; sialic acid binding Ig-like lectin H; Siglec; Siglech; Siglec-H; SIGLEC-like protein

more info or order :

Invitrogen product webpage