alpha Tubulin Monoclonal Antibody (DM1A)
Invitrogen
catalog: 62204
mouse monoclonal (DM1A)
reactivity: Leishmania infantum, Domestic guinea pig, African green monkey, hamsters, human, mouse, rat, dog, chicken, cow, pig, fruit fly, sheep, common platanna
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 119
quantity: 100µL
price: US 199
to the supplier
alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-22060
rabbit polyclonal
reactivity: human, mouse, rat, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 4

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells using PA5-22060 anti-Tubulin alpha 1A antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded SAS xenograft tissue using PA5-22060 anti-alpha Tubulin 1A antibody at 1:500 dilution.
quantity: 100µL
price: US 390
to the supplier
alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-19489
rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat, dog, chicken, cow, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 4

Immunoprecipitation of acetylated alpha-Tubulin was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an alpha-Tubulin polyclonal antibody (Product # PA5-19489) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

Western blot analysis of HeLa Whole Cell Lysate using PA5-19489, alpha Tubulin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19489 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19489 staining of A431 Whole Cell Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Immunofluorescent staining of HeLa cells using PA5-19489, anti-Alpha Tubulin antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
quantity: 50µg
price: US 390
to the supplier
alpha Tubulin Monoclonal Antibody (YOL1/34)
Invitrogen
catalog: MA1-80189
rat monoclonal (YOL1/34)
reactivity: Saccharomyces cerevisiae S288c, Candida albicans, human, mouse, rat, dog
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 3
quantity: 500µg
price: US 409
to the supplier
alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-29444
rabbit polyclonal
reactivity: human, mouse, rat, chicken, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western Blot analysis using anti-alpha Tubulin 4a Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG. Primary antibody (PA5-29444) diluted at 1:10000

Confocal immunofluorescence analysis of methanol-fixed HeLa using alpha Tubulin 4a (PA5-29444) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with MA1-25054 (red) at 1:2500.

Immunohistochemical analysis of paraffin-embedded U373 xenograft using alpha Tubulin 4a (PA5-29444) antibody at 1:500 dilution.
quantity: 100µL
price: US 390
to the supplier
alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-39836
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100µg
price: US 345
to the supplier
alpha Tubulin Monoclonal Antibody (GT114)
Invitrogen
catalog: MA5-31466
mouse monoclonal (GT114)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity: 100µL
price: US 345
to the supplier
alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-16891
rabbit polyclonal
reactivity: Domestic guinea pig, human, mouse, rat, chicken, cow, pig, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 500µL
price: US 390
to the supplier