GRP94 Monoclonal Antibody (9G10)
Invitrogen
catalog: MA3-016
rat monoclonal (9G10)
reactivity: Chinese hamsters, human, mouse, rat, chicken, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 24

Immunofluorescent analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) (Product# MA3-016 ) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 (Product# MA3-016 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse breast tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Glucose Regulated Protein 94 (MA3-016) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100 uL
price: US 491.00
to the supplier
KDEL Polyclonal Antibody
Invitrogen
catalog: PA1-013
domestic rabbit polyclonal
reactivity: Chinese hamsters, human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 14
quantity: 100 ug
price: US 451.00
to the supplier
GRP94 Monoclonal Antibody (9G10.F8.2)
Invitrogen
catalog: MA5-14592
rat monoclonal (9G10.F8.2)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit, domestic horse
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 4
quantity: 500 uL
price: US 474.00
to the supplier
KDEL Polyclonal Antibody, Alexa Fluor 488
Invitrogen
catalog: PA1-013-A488
domestic rabbit polyclonal
reactivity: Chinese hamsters, human, mouse, rat
conjugate: AF488
application: immunocytochemistry
citations: 2
quantity: 50 uL
price: US 419.00
to the supplier
KDEL Polyclonal Antibody, Alexa Fluor 555
Invitrogen
catalog: PA1-013-A555
domestic rabbit polyclonal
reactivity: Chinese hamsters, human, mouse, rat
conjugate: Alexa Fluor 555
application: immunocytochemistry
citations: 1
quantity: 50 uL
price: US 397.00
to the supplier
KDEL Polyclonal Antibody, Alexa Fluor 647
Invitrogen
catalog: PA1-013-A647
domestic rabbit polyclonal
reactivity: Chinese hamsters, human, mouse, rat
conjugate: AF647
application: immunocytochemistry
citations: 1
quantity: 50 uL
price: US 397.00
to the supplier
GRP94 Monoclonal Antibody (9G10), APC
Invitrogen
catalog: MA5-45096
rat monoclonal (9G10)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit, domestic horse
conjugate: APC
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
quantity: 200 ug
price: US 454.00
to the supplier
GRP94 Monoclonal Antibody (9G10), FITC
Invitrogen
catalog: MA5-45097
rat monoclonal (9G10)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit, domestic horse
conjugate: FITC
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
quantity: 200 ug
price: US 444.00
to the supplier
GRP94 Monoclonal Antibody (9G10), PerCP
Invitrogen
catalog: MA5-45098
rat monoclonal (9G10)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit, domestic horse
conjugate: PerCP
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
quantity: 200 ug
price: US 454.00
to the supplier