mouse monoclonal (E.665.10)
reactivity: Chinese hamsters, human, mouse, rat, zebrafish
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 3
reactivity: Chinese hamsters, human, mouse, rat, zebrafish
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 3
Flow cytometric analysis of Phospho-JNK on Jurkat cells left untreated (green histogram) or treated with anisomycin (blue histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-15228) at a dilution of 1:200 for 1 hour at room temperature. As a negative control anisomycin-treated cells were stained with an isotype control (red histogram). Following staining with either MA5-15228 or isotype control, cells were stained with a fluorescently-conjugated anti-mouse IgG secondary antibody and analyzed on a flow cytometer.
Immunofluorescent analysis of Phospho-JNK (green) in HeLa cells either left untreated (left panel) or treated with 25ug/ml anisomycin (right panel) for 25 min. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-15228) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
quantity: 200 µL
price: US 496
to the supplier
domestic rabbit polyclonal
reactivity: Chinese hamsters, human, mouse, rat, bovine, zebrafish
application: western blot
citations: 1
reactivity: Chinese hamsters, human, mouse, rat, bovine, zebrafish
application: western blot
citations: 1
domestic rabbit monoclonal (H.715.7)
reactivity: Chinese hamsters, human, mouse, rat
application: western blot, immunoprecipitation
reactivity: Chinese hamsters, human, mouse, rat
application: western blot, immunoprecipitation
domestic rabbit monoclonal (R.532.8)
reactivity: Chinese hamsters, human, mouse, rat
modification: phosphorylated
application: western blot
reactivity: Chinese hamsters, human, mouse, rat
modification: phosphorylated
application: western blot