mouse monoclonal (10E9)
reactivity: human, mouse, dog
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1
reactivity: human, mouse, dog
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1
Immunofluorescent analysis of Phospho-CREB pSer133 (green) in HeLa cells either left untreated (left panel) or treated with 1uM Camptothecin (right panel) for 20 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-CREB pSer133 monoclonal antibody (Product # MA1-114) at a concentration of 4ug/ml for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight Instrument at 20X magnification.
quantity: 100µg
price: US 560
to the supplier
rabbit polyclonal
reactivity: Domestic guinea pig, human, mouse, rat, dog, cow, zebrafish , rabbit, equine
application: western blot, immunohistochemistry
reactivity: Domestic guinea pig, human, mouse, rat, dog, cow, zebrafish , rabbit, equine
application: western blot, immunohistochemistry
rabbit polyclonal
reactivity: Domestic guinea pig, human, mouse, rat, dog, cow, zebrafish , sheep, rabbit, equine
application: western blot, immunohistochemistry
reactivity: Domestic guinea pig, human, mouse, rat, dog, cow, zebrafish , sheep, rabbit, equine
application: western blot, immunohistochemistry
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section