Phosphotyrosine Monoclonal Antibody (pY20), HRP | Invitrogen 03-7720 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phosphotyrosine Monoclonal Antibody (pY20), HRP

catalog :

03-7720

quantity :

100 ug

price :

US 451.00

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

PY20

application :

western blot, ELISA, immunocytochemistry

citations: 10

Reference
Alonso Morales A, GonzÃ¡lez LÃ³pez L, CÃ¡zares Raga F, CortÃ©s MartÃnez L, Torres MonzÃ³n J, Gallegos PÃ©rez J, et al. Protein phosphorylation during Plasmodium berghei gametogenesis. Exp Parasitol. 2015;156:49-60 pubmed publisher
Li S, Bhave D, Chow J, Riera T, Schlee S, Rauch S, et al. Quantitative analysis of receptor tyrosine kinase-effector coupling at functionally relevant stimulus levels. J Biol Chem. 2015;290:10018-36 pubmed publisher
de Kreuk B, Anthony E, Geerts D, Hordijk P. The F-BAR protein PACSIN2 regulates epidermal growth factor receptor internalization. J Biol Chem. 2012;287:43438-53 pubmed publisher
Paul J, Maiti K, Read M, Hure A, Smith J, Chan E, et al. Phasic phosphorylation of caldesmon and ERK 1/2 during contractions in human myometrium. PLoS ONE. 2011;6:e21542 pubmed publisher
Crockett J, Newman D, Newman P. PECAM-1 functions as a negative regulator of laminin-induced platelet activation. J Thromb Haemost. 2010;8:1584-93 pubmed publisher
Favier B, Alam A, Barron P, Bonnin J, Laboudie P, Fons P, et al. Neuropilin-2 interacts with VEGFR-2 and VEGFR-3 and promotes human endothelial cell survival and migration. Blood. 2006;108:1243-50 pubmed
Alam A, Herault J, Barron P, Favier B, Fons P, Delesque Touchard N, et al. Heterodimerization with vascular endothelial growth factor receptor-2 (VEGFR-2) is necessary for VEGFR-3 activity. Biochem Biophys Res Commun. 2004;324:909-15 pubmed
Kenny T, Keen C, Jones P, Kung H, Schmitz H, Gershwin M. Cocoa procyanidins inhibit proliferation and angiogenic signals in human dermal microvascular endothelial cells following stimulation by low-level H2O2. Exp Biol Med (Maywood). 2004;229:765-71 pubmed
Friedrichsen B, Richter H, Hansen J, Rhodes C, Nielsen J, Billestrup N, et al. Signal transducer and activator of transcription 5 activation is sufficient to drive transcriptional induction of cyclin D2 gene and proliferation of rat pancreatic beta-cells. Mol Endocrinol. 2003;17:945-58 pubmed
Sayos J, Nguyen K, Wu C, Stepp S, Howie D, Schatzle J, et al. Potential pathways for regulation of NK and T cell responses: differential X-linked lymphoproliferative syndrome gene product SAP interactions with SLAM and 2B4. Int Immunol. 2000;12:1749-57 pubmed

product information

Product Type :

Antibody

Product Name :

Phosphotyrosine Monoclonal Antibody (pY20), HRP

Catalog # :

03-7720

Quantity :

100 ug

Price :

US 451.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Chemical

Applications :

ELISA: 1 ug/mL, Western Blot: Assay-dependent

Species :

Chemical

Clone :

pY20

Isotype :

IgG2b

Storage :

4 C

Description :

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Immunogen :

Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1 ug/mL, Western Blot: Assay-dependent

Aliases :

Phosphotyrosine; pTyr; pY