vasodilator stimulated phosphoprotein [VASP] | Immunoglobe 0016-blk product information

product summary

company name :

Immunoglobe

product type :

antibody

product name :

vasodilator stimulated phosphoprotein [VASP]

catalog :

0016-blk

quantity :

500 µl, 1.0 mg; 5 vi

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IE273

reactivity :

human, cow

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Immunoglobe product webpage

citations: 44

Reference
Iyu D, Glenn J, White A, Fox S, Heptinstall S. Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist. Arterioscler Thromb Vasc Biol. 2011;31:416-22 pubmed publisher
Schulz E, Tsilimingas N, Rinze R, Reiter B, Wendt M, Oelze M, et al. Functional and biochemical analysis of endothelial (dys)function and NO/cGMP signaling in human blood vessels with and without nitroglycerin pretreatment. Circulation. 2002;105:1170-5 pubmed
Lohmann S, Walter U. Tracking functions of cGMP-dependent protein kinases (cGK). Front Biosci. 2005;10:1313-28 pubmed
Pena E, Padro T, Molins B, Vilahur G, Badimon L. Proteomic signature of thrombin-activated platelets after in vivo nitric oxide-donor treatment: coordinated inhibition of signaling (phosphatidylinositol 3-kinase-γ, 14-3-3ζ, and growth factor receptor-bound protein 2) and cytoskeleton protein translocati. Arterioscler Thromb Vasc Biol. 2011;31:2560-9 pubmed publisher
Iyu D, Glenn J, White A, Fox S, Dovlatova N, Heptinstall S. P2Y₁₂ and EP3 antagonists promote the inhibitory effects of natural modulators of platelet aggregation that act via cAMP. Platelets. 2011;22:504-15 pubmed publisher
Molins B, Pena E, de la Torre R, Badimon L. Monomeric C-reactive protein is prothrombotic and dissociates from circulating pentameric C-reactive protein on adhered activated platelets under flow. Cardiovasc Res. 2011;92:328-37 pubmed publisher
Mischnik M, Boyanova D, Hubertus K, Geiger J, Philippi N, Dittrich M, et al. A Boolean view separates platelet activatory and inhibitory signalling as verified by phosphorylation monitoring including threshold behaviour and integrin modulation. Mol Biosyst. 2013;9:1326-39 pubmed publisher
Zhang L, Wang T, Wen X, Wei Y, Peng X, Li H, et al. Effect of matrine on HeLa cell adhesion and migration. Eur J Pharmacol. 2007;563:69-76 pubmed
Geiger J, Teichmann L, Grossmann R, Aktas B, Steigerwald U, Walter U, et al. Monitoring of clopidogrel action: comparison of methods. Clin Chem. 2005;51:957-65 pubmed
Iyu D, Glenn J, White A, Fox S, van Giezen H, Nylander S, et al. Mode of action of P2Y(12 ) antagonists as inhibitors of platelet function. Thromb Haemost. 2011;105:96-106 pubmed publisher
August M, Wingerter O, Oelze M, Wenzel P, Kleschyov A, Daiber A, et al. Mechanisms underlying dysfunction of carotid arteries in genetically hyperlipidemic rabbits. Nitric Oxide. 2006;15:241-51 pubmed
Geiger J, Brandmann T, Hubertus K, Tjahjadi B, Schinzel R, Walter U. A protein phosphorylation-based assay for screening and monitoring of drugs modulating cyclic nucleotide pathways. Anal Biochem. 2010;407:261-9 pubmed publisher
Mirone V, d'Emmanuele di Villa Bianca R, Mitidieri E, Imbimbo C, Fusco F, Verze P, et al. Platelet cyclic guanosine monophosphate as a biomarker of phosphodiesterase type 5 inhibitor efficacy in the treatment of erectile dysfunction: a randomized placebo-controlled study. Eur Urol. 2009;56:1067-73 pubmed publisher
Maruoka M, Sato M, Yuan Y, Ichiba M, Fujii R, Ogawa T, et al. Abl-1-bridged tyrosine phosphorylation of VASP by Abelson kinase impairs association of VASP to focal adhesions and regulates leukaemic cell adhesion. Biochem J. 2012;441:889-99 pubmed publisher
Benz P, Blume C, Moebius J, Oschatz C, Schuh K, Sickmann A, et al. Cytoskeleton assembly at endothelial cell-cell contacts is regulated by alphaII-spectrin-VASP complexes. J Cell Biol. 2008;180:205-19 pubmed publisher
Smolenski A, Poller W, Walter U, Lohmann S. Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I. J Biol Chem. 2000;275:25723-32 pubmed
Anderson S, Behrendt B, Machesky L, Insall R, Nash G. Linked regulation of motility and integrin function in activated migrating neutrophils revealed by interference in remodelling of the cytoskeleton. Cell Motil Cytoskeleton. 2003;54:135-46 pubmed
Zhang Y, Han G, Fan B, Zhou Y, Zhou X, Wei L, et al. Green tea (-)-epigallocatechin-3-gallate down-regulates VASP expression and inhibits breast cancer cell migration and invasion by attenuating Rac1 activity. Eur J Pharmacol. 2009;606:172-9 pubmed publisher
Galler A, Garcia Arguinzonis M, Baumgartner W, Kuhn M, Smolenski A, Simm A, et al. VASP-dependent regulation of actin cytoskeleton rigidity, cell adhesion, and detachment. Histochem Cell Biol. 2006;125:457-74 pubmed
Dunkern T, Hatzelmann A. The effect of Sildenafil on human platelet secretory function is controlled by a complex interplay between phosphodiesterases 2, 3 and 5. Cell Signal. 2005;17:331-9 pubmed
Wei L, Muller S, Ouyang J, Stoltz J, Wang X. Changes of vasodilator-stimulated phosphoprotein (VASP) and its phosphorylation in endothelial cells exposed to laminar flow. Clin Hemorheol Microcirc. 2003;28:113-20 pubmed
Wang Y, Dong H, Zhu M, Ou Y, Zhang J, Luo H, et al. Icariin exterts negative effects on human gastric cancer cell invasion and migration by vasodilator-stimulated phosphoprotein via Rac1 pathway. Eur J Pharmacol. 2010;635:40-8 pubmed publisher
Tadokoro S, Nakazawa T, Kamae T, Kiyomizu K, Kashiwagi H, Honda S, et al. A potential role for α-actinin in inside-out αIIbβ3 signaling. Blood. 2011;117:250-8 pubmed publisher
Zhang D, Ouyang J, Wang N, Zhang Y, Bie J, Zhang Y. Promotion of PDGF-induced endothelial cell migration by phosphorylated VASP depends on PKA anchoring via AKAP. Mol Cell Biochem. 2010;335:1-11 pubmed publisher
Iyu D, Glenn J, White A, Johnson A, Fox S, Heptinstall S. The role of prostanoid receptors in mediating the effects of PGE(2) on human platelet function. Platelets. 2010;21:329-42 pubmed publisher
Mülsch A, Oelze M, Kloss S, Mollnau H, Töpfer A, Smolenski A, et al. Effects of in vivo nitroglycerin treatment on activity and expression of the guanylyl cyclase and cGMP-dependent protein kinase and their downstream target vasodilator-stimulated phosphoprotein in aorta. Circulation. 2001;103:2188-94 pubmed
Oelze M, Mollnau H, Hoffmann N, Warnholtz A, Bodenschatz M, Smolenski A, et al. Vasodilator-stimulated phosphoprotein serine 239 phosphorylation as a sensitive monitor of defective nitric oxide/cGMP signaling and endothelial dysfunction. Circ Res. 2000;87:999-1005 pubmed
Blume C, Benz P, Walter U, Ha J, Kemp B, Renne T. AMP-activated protein kinase impairs endothelial actin cytoskeleton assembly by phosphorylating vasodilator-stimulated phosphoprotein. J Biol Chem. 2007;282:4601-12 pubmed
Mollnau H, Schulz E, Daiber A, Baldus S, Oelze M, August M, et al. Nebivolol prevents vascular NOS III uncoupling in experimental hyperlipidemia and inhibits NADPH oxidase activity in inflammatory cells. Arterioscler Thromb Vasc Biol. 2003;23:615-21 pubmed
Bachmann C, Fischer L, Walter U, Reinhard M. The EVH2 domain of the vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding, and actin bundle formation. J Biol Chem. 1999;274:23549-57 pubmed
Dugina V, Zwaenepoel I, Gabbiani G, Clement S, Chaponnier C. Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity. J Cell Sci. 2009;122:2980-8 pubmed publisher
Niebuhr K, Ebel F, Frank R, Reinhard M, Domann E, Carl U, et al. A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. EMBO J. 1997;16:5433-44 pubmed
Birschmann I, Mietner S, Dittrich M, Pfrang J, Dandekar T, Walter U. Use of functional highly purified human platelets for the identification of new proteins of the IPP signaling pathway. Thromb Res. 2008;122:59-68 pubmed
Glenn J, White A, Iyu D, Heptinstall S. PGE(2) reverses G(s)-mediated inhibition of platelet aggregation by interaction with EP3 receptors, but adds to non-G(s)-mediated inhibition of platelet aggregation by interaction with EP4 receptors. Platelets. 2012;23:344-51 pubmed publisher
Lambrechts A, Verschelde J, Jonckheere V, Goethals M, Vandekerckhove J, Ampe C. The mammalian profilin isoforms display complementary affinities for PIP2 and proline-rich sequences. EMBO J. 1997;16:484-94 pubmed
Warnholtz A, Ostad M, Velich N, Trautmann C, Schinzel R, Walter U, et al. A single loading dose of clopidogrel causes dose-dependent improvement of endothelial dysfunction in patients with stable coronary artery disease: results of a double-blind, randomized study. Atherosclerosis. 2008;196:689-95 pubmed
Gustavsson A, Yuan M, Fallman M. Temporal dissection of beta1-integrin signaling indicates a role for p130Cas-Crk in filopodia formation. J Biol Chem. 2004;279:22893-901 pubmed
Wangorsch G, Butt E, Mark R, Hubertus K, Geiger J, Dandekar T, et al. Time-resolved in silico modeling of fine-tuned cAMP signaling in platelets: feedback loops, titrated phosphorylations and pharmacological modulation. BMC Syst Biol. 2011;5:178 pubmed publisher
van der Ven P, Ehler E, Vakeel P, Eulitz S, Schenk J, Milting H, et al. Unusual splicing events result in distinct Xin isoforms that associate differentially with filamin c and Mena/VASP. Exp Cell Res. 2006;312:2154-67 pubmed
Mollnau H, Wendt M, Szocs K, LASSEGUE B, Schulz E, Oelze M, et al. Effects of angiotensin II infusion on the expression and function of NAD(P)H oxidase and components of nitric oxide/cGMP signaling. Circ Res. 2002;90:E58-65 pubmed
Aggarwal S, Gross C, Kumar S, Datar S, Oishi P, Kalkan G, et al. Attenuated vasodilatation in lambs with endogenous and exogenous activation of cGMP signaling: role of protein kinase G nitration. J Cell Physiol. 2011;226:3104-13 pubmed publisher
Iyu D, Jüttner M, Glenn J, White A, Johnson A, Fox S, et al. PGE1 and PGE2 modify platelet function through different prostanoid receptors. Prostaglandins Other Lipid Mediat. 2011;94:9-16 pubmed publisher
Obergfell A, Judd B, del Pozo M, Schwartz M, Koretzky G, Shattil S. The molecular adapter SLP-76 relays signals from platelet integrin alphaIIbbeta3 to the actin cytoskeleton. J Biol Chem. 2001;276:5916-23 pubmed
Ibarra-Alvarado C, Galle J, Melichar V, Mameghani A, Schmidt H. Phosphorylation of blood vessel vasodilator-stimulated phosphoprotein at serine 239 as a functional biochemical marker of endothelial nitric oxide/cyclic GMP signaling. Mol Pharmacol. 2002;61:312-9 pubmed

product information

CATALOG NO :

0016-blk

NAME (Link To Web Site) :

IE273, carrier-free

SPECIFICITY :

vasodilator stimulated phosphoprotein [VASP]

OTHER NAMES :

vasodilator-stimulated phosphoprotein

UniProt / Swiss-Prot # :

P50552, B2RBT9, Q6PIZ1, Q93035

CLONE/Poly-clonal :

monoclonal, IE273

HOST SPECIES :

MOUSE

ISOTYPE :

IgG1

reactive species :

human, pig, bovine, sheep, rabbit; by inference (sequence idetity: cat, African elephant, manatee)

FORMAT/CONJUGATES :

purified, carrier-free

SIZE :

500 µl, 1.0 mg; 5 vials or more

DESCRIPTION :

carrier-free; comes with a free positive control: human platelet protein (lysate); antibody raised against full-length protein; detailed information on epitope available; works with formaldehyde fixed cells; the antibody recognizes both the 46 kDa (Ser-157 dephospho) and 50 kDa (Ser-157 phospho) form of VASP

APPLICATIONS :

WB - Western blot, immunoblot, IF - immunofluorescence, IHC - immuno-histochemistry, IP - immunoprecipitation, ELISA

SAMPLE APPLICATIONS :

Basic and Clinical Research Applications

SUGGESTED DILUTIONS :

WB: 0.1 μg/ml (dilution 1:20,000), IF: 1-5 µg/ml (1:400-2,000), IP

[PubMed-Id; PMID] :

21351102, 12529859, 16597505, 10438535, 18195108, 17931695, 17082196, 19638415, 15567064, 16267652, 20708596, 15817818, 22436052, 15044442, 11809855, 21591981, 21106949, 20941457, 20433310, 21095237, 9034331, 15769627, 22014333, 19781847, 23463387, 21859817, 12692005, 11884382, 11331261, 12652017, 9312002, 11113155, 11090544, 21836071, 11889009, 10851246, 20940419, 16631741, 20307526, 22034949, 17214996, 12652017, 19711176, 17343841, 19171136, 15567064

Updated :

5/6/13

more info or order :

Immunoglobe product webpage