PKC alpha Antibody [MC5]: Horseradish peroxidase (HRP) | ImmuQuest IQ625HRP product information

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product summary

company name :

ImmuQuest

product type :

antibody

product name :

PKC alpha Antibody [MC5]: Horseradish peroxidase (HRP)

catalog :

IQ625HRP

quantity :

50 ul

price :

380 USD

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

'MC5

reactivity :

human, mouse, rat, dog, cow

application :

western blot, ELISA, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

Product Name :

PKC alpha Antibody [MC5]: Horseradish peroxidase (HRP)

Target Antigen :

PKC alpha

Primary Description :

Mouse anti PKC alpha [MC5]: Horseradish peroxidase (HRP)

Catalogue Number :

IQ625HRP

Quantity :

50 ul

Concentration :

1mg/ml

Clone :

'MC5

Host :

Mouse

Isotype :

IgG2a

Immunogen :

Purified bovine brain protein kinase C

Myeloma/fusion partners :

Spleen cells from immunized Balb/c mice were fused with the P3X63Ag8 myeloma cell line

Specificity :

This antibody recognises protein kinase C alpha (PKC alpha). Protein Kinase C is a family of serine/threonine protein kinases that are activated by Ca2+, diacylglycerol and membrane phospholipids (phosphatidylserine). The antibody MC5 blocks partial proteolysis of PKC by trypsin in vitro. The antibody MC5 recognises an epitope within the variable region V3 of PKC alpha.

Species Reactivity :

Mouse, Rat, Human, Sheep, Rabbit, Dog, Cow, Pig

Purification :

Purified on protein A from tissue culture supernatant.

Format :

Purified IgG conjugated to Horseradish Peroxidase using Innova Biosciences Lightning-LinkÂ®, supplied in Phosphate buffered saline (PBS) containing 0.01% Thiomersal

Applications :

ELISA, IHC-Fr, IHC-P, IP, WB

Dilutions :

Optimal antibody dilution should be determined by titration

References :

Young S, et al. A monoclonal antibody recognising the site of limited proteolysis of protein kinase C. Inhibition of down-regulation in vivo. Eur J Biochem. 1988.173(1):247-52. PMID:2451608 http://www.ncbi.nlm.nih.gov/pubmed/2451608

Database links :

Entrez Gene: 282001 Cow; Entrez Gene: 5578 Human; Entrez Gene: 18750 Mouse; Entrez Gene: 100037720 Rabbit; Entrez Gene: 24680 Rat; Omim: 176960 Human; SwissProt: P04409 Cow; SwissProt: P17252 Human; SwissProt: P20444 Mouse; SwissProt: P10102 Rabbit; SwissProt: P05696 Rat; Unigene: 531704 Human; Unigene: 708867 Human; Unigene: 222178 Mouse; Unigene: 207908 Rat

Research areas :

Angiogenesis; Apoptosis

Also known as :

AAG6; aging-associated gene 6; KPCA; MGC129900; MGC129901; PKC-A; PKC-alpha; PKCA; PRKACA; PRKCA; Protein kinase C alpha type; protein kinase C, alpha

Price (Pounds) :

245

Price (Dollars) :

380 USD

Price (Euros) :

366