RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - ChIP Grade

product summary

company name :

ImmuQuest

product type :

antibody

product name :

RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - ChIP Grade

catalog :

IQ585

quantity :

0.1 ml

price :

345 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

'4H8

reactivity :

human, mouse, fruit fly, thale cress, Caenorhabditis elegans

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation

more info or order :

ImmuQuest product webpage

product information

Product Name :

RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - ChIP Grade

Target Antigen :

RNA polymerase II CTD repeat YSPTSPS (phospho S5)

Primary Description :

Mouse Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - ChIP Grade

Catalogue Number :

IQ585

Quantity :

0.1 ml

Concentration :

0.8mg/ml

Clone :

'4H8

Host :

Mouse

Isotype :

IgG1

Immunogen :

Synthetic peptide: YSPTSpPS (Human). The sequence is repeated multiple times in the C-terminal domain of RNA polymerase II

Myeloma/fusion partners :

sp2/0 Ag.14

Specificity :

Recognises the C-terminal repeat of the largest subunit of RNA polymerase II. It recognises both unphosphorylated and phosphorylated forms

Species Reactivity :

Mouse, Human, Saccharomyces cerevisiae, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Schizosaccharomyces pombe, African Green Monkey

Purification :

Protein A purified

Format :

Purified antibody in PBS + 0.1% sodium azide

Applications :

Flow Cyt, ICC/IF, ChIP, ChIP/Chip, WB, ELISA, IP

Dilutions :

Optimal antibody dilution should be determined by titration

References :

The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase Yart A et al. Cell Biol. 2005 Jun;25(12):5052-60ChIP ; Human PMID: 15923622 Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation Kristjuhan A, Wittschieben BO, Walker J, Roberts D, Cairns BR, Svejstrup JQ. ( Proc Natl Acad Sci U S A,2003) PUBMED Keren-Shaul H et al. Pre-mRNA splicing is a determinant of nucleosome organization. PLoS One 8:e53506 (2013). WB, ChIP ; Human PUBMED Schmucker AC et al. Distal Interleukin-1ÃŸ (IL-1ÃŸ) Response Element of Human Matrix Metalloproteinase-13 (MMP-13) Binds Activator Protein 1 (AP-1) Transcription Factors and Regulates Gene Expression. J Biol Chem 287:1189-97 (2012). ChIP ; Human PUBMED Davidson L et al. Co-transcriptional degradation of aberrant pre-mRNA by Xrn2. EMBO J 31:2566-78 (2012). ChIP PUBMED Harrigan JA et al. Replication stress induces 53BP1-containing OPT domains in G1 cells. J Cell Biol 193:97-108 (2011). ICC/IF ; Human PUBMED Chen X et al. Sequential changes at differentiation gene promoters as they become active in a stem cell lineage. Development 138:2441-50 (2011) PUBMED

Database links :

Entrez Gene: 177190 Caenorhabditis elegans Entrez Gene: 32100 Fruit fly (Drosophila melanogaster) Entrez Gene: 5430 Human Entrez Gene: 20020 Mouse Entrez Gene: 851415 Saccharomyces cerevisiae Omim: 180660 Human SwissProt: P18616 Arabidopsis thaliana SwissProt: P16356 Caenorhabditis elegans SwissProt: P04052 Fruit fly (Drosophila melanogaster) SwissProt: P24928 Human SwissProt: P08775 Mouse Unigene: 2925 Fruit fly (Drosophila melanogaster) Unigene: 270017 Human Unigene: 16533 Mouse

Price (Pounds) :

226

Price (Dollars) :

345 USD

Price (Euros) :

338

more info or order :

ImmuQuest product webpage