HEF1/NEDD-9 Antibody [2G9] | ImmuQuest IQ296 product information

product summary

company name :

ImmuQuest

product type :

antibody

product name :

HEF1/NEDD-9 Antibody [2G9]

catalog :

IQ296

quantity :

50 ul

price :

241 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2G9

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

ImmuQuest product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 1a	Zhong J, Baquiran J, Bonakdar N, Lees J, Ching Y, Pugacheva E, et al. NEDD9 stabilizes focal adhesions, increases binding to the extra-cellular matrix and differentially effects 2D versus 3D cell migration. PLoS ONE. 2012;7:e35058 pubmed publisher
immunoprecipitation; mouse western blot; mouse	Baquiran J, Bradbury P, O Neill G. Tyrosine Y189 in the substrate domain of the adhesion docking protein NEDD9 is conserved with p130Cas Y253 and regulates NEDD9-mediated migration and focal adhesion dynamics. PLoS ONE. 2013;8:e69304 pubmed publisher
	Kondo S, Iwata S, Yamada T, Inoue Y, Ichihara H, Kichikawa Y, et al. Impact of the integrin signaling adaptor protein NEDD9 on prognosis and metastatic behavior of human lung cancer. Clin Cancer Res. 2012;18:6326-38 pubmed publisher
	Bradbury P, Mahmassani M, Zhong J, Turner K, Paul A, Verrills N, et al. PP2A phosphatase suppresses function of the mesenchymal invasion regulator NEDD9. Biochim Biophys Acta. 2012;1823:290-7 pubmed publisher
	Bradshaw L, Zhong J, Bradbury P, Mahmassani M, Smith J, Ammit A, et al. Estradiol stabilizes the 105-kDa phospho-form of the adhesion docking protein NEDD9 and suppresses NEDD9-dependent cell spreading in breast cancer cells. Biochim Biophys Acta. 2011;1813:340-5 pubmed publisher
	Bargon S, Gunning P, O Neill G. The Cas family docking protein, HEF1, promotes the formation of neurite-like membrane extensions. Biochim Biophys Acta. 2005;1746:143-54 pubmed
	Natarajan M, Stewart J, Golemis E, Pugacheva E, Alexandropoulos K, Cox B, et al. HEF1 is a necessary and specific downstream effector of FAK that promotes the migration of glioblastoma cells. Oncogene. 2006;25:1721-32 pubmed

product information

Product Name :

HEF1/NEDD-9 Antibody [2G9]

Target Antigen :

HEF1/NEDD-9

Primary Description :

Mouse Anti-HEF1 HEF1/NEDD-9 [2G9]

Catalogue Number :

IQ296

Quantity :

50 ul

Concentration :

1mg/ml

Clone :

2G9

Host :

Mouse¬†

Isotype :

IgG1 kappa

Immunogen :

Fusion protein, corresponding to amino acids 82-398 of Human HEF1.

Myeloma/fusion partners :

SP2/0

Species Reactivity :

Mouse, Rat, Human

Purification :

Protein G

Format :

Purified antibody containing PBS 0.1% sodium azide;

Applications :

Immunofluorescence,Immunohistochemistry (paraffin sections),Western blot,Immunoprecipitation

Dilutions :

Optimal antibody dilution should be determined by titration, however as a guideline try;¬†WB: 1/2000 - 1/6000. Predicted molecular weight: 93 kDa.IP: 1/1000.ICC/IF: Use at an assay dependent dilution. PubMed: 19376971IHC-Fr: Use at an assay dependent dilution. PubMed: 19464348Flow Cyt: Use 1¬µg for 106 cells

Also known as :

CAS L antibody;Cas like docking antibody;CASL antibody;Crk associated substrate related proteinantibody;dJ49G10.2 antibody;dJ761I2.1 antibody;Enhancer of filamentation 1 antibody;HEF 1 antibody;NEDD 9antibody;NEDD9 antibody;NEDD9 protein antibody;Neural cell expressed developmentally down regulated 9antibody;Neural precursor cell expressed developmentally down regulated 9 antibody;NY REN 12 antigenantibody;p105 antibody;Protein NEDD9 antibody

List Price (Pounds) :

241 USD

Pubmed ID :

30267961, 30579603, 31801619, 29773902, 29519561, 28194179, 29084958, 29371931, 26893853, 27805249, 24514042, 24058594, 22328516, 22665056, 22968701, 21829474, 19587023, 19464348, 19376971, 18282814, 18984162, 16814714, 23037767, 22061964, 22509381, 21317929, 21145356, 19539609, 19648964, 16288224, 16344118, 11438665

SwissProt ID :

q14511, o35177, Q14511, O35177

more info or order :

ImmuQuest product webpage