This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Hytest
product type :
protein
product name :
Thermus aquaticus DNA Polymerase, Recombinant
catalog :
7T1
citations: 2
Reference
Kiviniemi M, Hermann R, Nurmi J, Ziegler A, Knip M, Simell O, et al. A high-throughput population screening system for the estimation of genetic risk for type 1 diabetes: an application for the TEDDY (the Environmental Determinants of Diabetes in the Young) study. Diabetes Technol Ther. 2007;9:460-72 pubmed
Kiviniemi M, Nurmi J, Lövgren T, Ilonen J. Locked nucleic acid (LNA) probes in high-throughput genetic analysis: application to an assay for type 1 diabetes-related HLA-DQB1 alleles. Clin Biochem. 2005;38:1015-22 pubmed
product information
Product Name :
Thermus aquaticus DNA Polymerase, Recombinant
Catalog Number :
7T1
Spec:Description :
Taq DNA Polymerase (recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus strain YT-1) consists of a single polypeptide chain with a molecular weight 94 kDa. It is a highly processive 5'-3' DNA polymerase lacking 3'-5' exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- and exonucleases. This Taq DNA polymerase has a nontemplate-dependent activity, which adds a single deoxyadenosine (A) to the 3' ends of PCR products (3'-A overhangs). This property allows easily and efficiently to ligate the PCR products in TA cloning vectors. Amplification of target DNA fragments from 100 bp to 4 Kb can be achieved with this enzyme
Categorization :
Antibodies
Spec:Specifications/Features :
Buffer: Storage and dilution buffer: 20 mM HEPES, pH 7.9, 125 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5 % Tween 20, 50 % glycerol. Reaction buffer 10x (Mg 2+ minus): 670 mM Tris-HCl, pH 8.8, 166 mM (NH4) 2 SO 4 0.1 % Tween 20. Other common PCR buffers for Taq-DNA polymerases can be used as well. Optimal concentration of MgCl2 is 1.0-2.5 mM.
Spec:Concentration :
5 units/ l
Spec:Brand/Trademark :
HyTest Ltd
company information
Hytest
Intelligate 6th floor
Joukahaisenkatu 6, 20520, Turku
hytest@hytest.fi
http://www.hytest.fi
358-2-512-0900
headquarters: Finland
HyTest Ltd. is a biotechnology-oriented company based in Turku, Finland, that manufactures and markets high-quality immunological reagents for industrial and research applications. The product portfolio includes cardiac markers, hormones and toxins, human proteins, neuroscience-related reagents, infectious and autoimmune disease reagents.