Fatty Acid Synthase | HUABIO R1706-8 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Fatty Acid Synthase

catalog :

R1706-8

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

All lanes: Western blot analysis of Fatty Acid Synthase with anti-Fatty Acid Synthase antibody (R1706-8) at 1:1,000 dilution. Lane 1: Wild-type Hela whole cell lysate. Lane 2: FASN knockdown Hela whole cell lysate. R1706-8 was shown to specifically react with FASN in wild-type Hela cells. Weakened band was observed when FASN knockdown samples were tested. Wild-type and FASN knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-FASN antibody (R1706-8, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

image 2 :

Western blot analysis of Fatty Acid Synthase on A549 cell lysate using anti-Fatty Acid Synthase antibody at 1/1,000 dilution.

image 3 :

ICC staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

R1706-8

Target name :

Fatty Acid Synthase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Fatty Acid Synthase aa 801-1050.

Uniprot id :

P49327>SwissProt: P49327 Human;SwissProt: P19096 Mouse;SwissProt: P12785 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

A549, MCF-7, Hela, human prostate tissue, human breast cancer tissue.

Molecular wt :

273 kDa

Subcellular location :

Cytoplasm. Melanosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1706-8_4.jpg

Pic legend4 :

ICC staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1706-8_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1706-8_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage