Tyrosinase | HUABIO R1706-4 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Tyrosinase

catalog :

R1706-4

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Tyrosinase on SK-MEL-28 cell lysates with Rabbit anti-Tyrosinase antibody (R1706-4) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1706-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.

image 3 :

ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

R1706-4

Target name :

Tyrosinase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Tyrosinase aa 51-100 / 529.

Uniprot id :

P14679>SwissProt: P14679 Human;SwissProt: P11344 Mouse;Entrez Gene: 308800 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SK-MEL-28 cell lysates, B16F1 cell lysates, B16F1, Hela, human melanoma tissue, mouse retina tissue, rat retina tissue, A431.

Molecular wt :

Predicted band size: 60 kDa

Subcellular location :

Melanosome membrane, Melanosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC:1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1706-4_4.jpg

Pic legend4 :

ICC staining Tyrosinase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1706-4_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-Tyrosinase antibody (R1706-4) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1706-4) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1706-4_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-Tyrosinase antibody (R1706-4) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1706-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1706-4_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-Tyrosinase antibody (R1706-4) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1706-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1706-4_8.jpg

Pic legend8 :

Flow cytometric analysis of A431 cells with Tyrosinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

more info or order :

HUABIO product webpage