DDB1 | HUABIO R1706-21 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DDB1

catalog :

R1706-21

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DDB1 on different cell lysate using anti-DDB1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse colon tissue Lane 2: PC-12 Lane 3: Siha

image 2 :

ICC staining DDB1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

R1706-21

Target name :

DDB1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human DDB1 aa 151-560 / 1,140.

Uniprot id :

Q16531>SwissProt: Q16531 Human;SwissProt: Q3U1J4 Mouse;SwissProt: Q9ESW0 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse colon tissue lysate, PC-12, Siha, A549, SH-SY5Y, SK-Br-3, K562, human breast, human kidney, rat brain.

Molecular wt :

Predicted band size: 127 kDa

Subcellular location :

Nucleus. Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:100-1:500 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1706-21_4.jpg

Pic legend4 :

ICC staining DDB1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1706-21_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1706-21_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1706-21_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1706-21_8.png

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-DDB1 antibody (R1706-21) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1706-21) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/R1706-21_9.jpg

Pic legend9 :

Flow cytometric analysis of K562 cells with DDB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage