Neurofilament | HUABIO R1706-17 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Neurofilament

catalog :

R1706-17

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Neurofilament on mouse brain tissue lysate using anti-Neurofilament antibody at 1/500 dilution.

image 2 :

Immunocytochemistry analysis of Neuro-2a cells labeling Neurofilament with Rabbit anti-Neurofilament antibody (R1706-17) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Neurofilament antibody (R1706-17) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin.

product information

SKU :

R1706-17

Target name :

Neurofilament

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Neurofilament aa 279-415.

Uniprot id :

P12036>SwissProt: P12036 Human;SwissProt: P19246 Mouse;SwissProt: P16884 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse brain tissue lysates, Neuro-2a, rat brain tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 112 kDa

Subcellular location :

Cytoplasm, Intermediate filament.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1706-17_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage