Jagged 1 | HUABIO R1706-10 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Jagged 1

catalog :

R1706-10

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of Raw264.7 cells labeling Jagged 1 with Rabbit anti-Jagged 1 antibody (R1706-10) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Jagged 1 antibody (R1706-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 2 :

ICC staining Jagged 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining Jagged 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

R1706-10

Target name :

Jagged 1

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Jagged 1 aa 90-144 / 1,218.

Uniprot id :

P78504>SwissProt: P78504 Human;SwissProt: Q9QXX0 Mouse;SwissProt: Q63722 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

293T, Hela, HepG2, Raw264.7, rat kidney tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 133 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1706-10_4.jpg

Pic legend4 :

ICC staining Jagged 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1706-10_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Jagged 1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1706-10_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Jagged 1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1706-10_7.jpg

Pic legend7 :

Flow cytometric analysis of HepG2 cells with Jagged 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage